Leptosphaeria purpurea Rehm, 1882

Mlčoch, Patrik & Matušinsky, Pavel, 2024, Phylogenetic and morphological revision sexual stages of the genus Paraphoma (Phaeosphaeriaceae) and next related species from clade of Ophiobolus-like (Phaeosphaeriaceae), Phytotaxa 663 (4), pp. 184-204 : 195

Leptosphaeria purpurea Rehm — Fig. 5

= Heptameria purpurea (Rehm) Cooke (1889) , Melanomma purpureum (Rehm) L. Holm (1957)

Sexual morph. Ascoma pseudothecial, globose, in numerous groups, subepidermal, 200–250 μm in diam., later semi-immersed to erumpent. Ostioles inconspicuous, small and conical. Ascomal wall is composed of several layers of pseudoparenchymatous cells, composed of brown to dark brown, tri- to polygonate cells of textura angularis, (5–)6.4– 9(–10.5) × (4.5–)5–7(–8) μm, N = 20. Asci bitunicate, 8–spored, cylindrical to cylindric-clavate, short pedicellate, (58–)60–70(–72) × 7.5–8.5(–9) μm, N=15. Ascospores narrowly fusiform, biseriate in ascus, hyaline to brownish, 3– septate, second cell from apex enlarged towards base, isodiametric, without gelatinous sheath, appendages and without -ornamentation, with several globose lipid drops, (19.5–)20–24(–25) × (4–)4.5–5(–5.5) μm, Q=4.3–5.1, Q AV =4.6, N = 20.

Asexual morph. Undetermined.

Culture characters. It was not cultivated.

Habitat. Dead stems of Asteraceae , primarily on the Artemisia vulgaris .

Distribution. Europe and North America ( Shoemaker 1984; Mlčoch 2021).

Material examined: SLOVAKIA: 1. col. P. Mlčoch, foothills of High Tatras, Hybe, Hybická tiesňava Natural Monument, unmown meadow with Tanaceto vulgaris-Artemisietum vulgaris association, 800 m a. s. l., 22. 8. 2019, on the dead stems of Artemisia vulgaris, GPS : 49.0872639N, 19.8961167E. ( BRNM 829154).

Notes. DNA could not be isolated from the described material in this study due to its poor condition. However, it can be presupposed that these species begin in the genus Paraphoma in the sensu stricto concept, the same as the next described taxa, where it’s morphologically related. Leptosphaeria purpurea is definite in the sensu stricto concept from the dead stems of Artemisia vulgaris (Saccardo P. A., Traverso G. B. & Trotter A. 1883) . Demarcation of separate species in the genus Paraphoma is strongly strict, with very little phenotypic variability in morphological characters. On the basis of these predictors, it can presuppose that collections, which are featured from various substrates in Shoemaker (1984) correspond with the sensu lato concept of Leptosphaeria purpurea . Shoemaker’s species concept is built on the study of Krieger’s collection from Cirisum lanceolatum (Fungi Saxonici, col. 1879), which was revised by Rehm, because the origin type material did not exist yet ( Shoemaker 1984). But his concept is a more similar description of Paraphoma rubicunda in this study than the original description of Leptosphaeria purpurea (taken over by Saccardo P. A., Traverso G. B. & Trotter A. 1883).

Figures

FIGURE 5. Paraphoma rubicunda (BRNM 829161) and Leptosphaeria purpurea (BRNM 829154). Leptosphaeria purpurea (A–F): A–B: asci; C–F: ascospores. Paraphoma rubicunda (G–O): G–J: ascoma; K–L: asci; M–O: ascospores. Scales: G: 1 mm; H–J: 300 μm; A–B, K–L: 10 μm; B–F, M–O: 5 μm.