Luteithermobacter gelatinilyticus, Park & Namirimu & Yang & Kwon, 2020

Park, Mi-Jeong, Namirimu, Teddy, Yang, Sung-Hyun & Kwon, Kae Kyoung, 2020, Description of Luteithermobacter gelatinilyticus gen. nov., sp. nov., and Paremcibacter congregatus gen. nov., comb. nov. via reclassification of the genus Emcibacter, International Journal of Systematic and Evolutionary Microbiology 70 (8), pp. 4691-4697 : 4695

DESCRIPTION OF LUTEITHERMOBACTER GELATINILYTICUS SP. NOV.

Luteithermobacter gelatinilyticus (ge.la.ti.ni.ly′ ti.cus. N.L. neut. n. gelatinum gelatin; Gr. masc. adj. lytikos able to dissolve; N.L. masc. adj. lyticus dissolving; N.L. masc. adj. gelatinilyticus gelatin-dissolving).

Cells are rod-shaped with a width of 0.4–0.5 µm and length of 1.5–2 µm. Colonies are 3 mm in diameter and pale yellow, with a circular, convex, entire edge, and a glistening surface on MA after 3 days of cultivation. Growth is observed at 35–59 °C (optimum 55 °C), at pH 6.0–9.0 (optimum pH 6.0) and with 1–9% (w/v) NaCl (optimum 2.5–3.5%) in the presence of KCl, MgCl 2 and CaCl 2. Nitrate is reduced to nitrite. Negative for indole production and cannot assimilate aesculin, p -NP(nitrophenyl)-galactopyranoside or urea. However, 2-naphthyl phosphate, 2-naphthyl butyrate, 2-naphthyl caprylate, L-leucyl-2-naphthylamide,L-valyl-2-naphthylamide, L -cystyl-2-naphthylamide, 2-naphthyl phosphate, naphthol-AS- BI- phosphate, dextrin, cellobiose, turanose, lactose, D-fructose, D -sorbitol, D-arabitol, myo -inositol,D-fructose 6-phosphate, D -aspartic acid, gelatin, L-arginine,L-histidine,L-serine, pectin, D -galacturonic acid,L-galactonic acid lactone,D-glucuronic acid, glucuronamide, p -hydroxy-phenylacetic acid, methyl pyruvate, D-lactic acid methyl ester, α-ketobutyric acid, acetoacetic acid, propionic acid, acetic acid and formic acid can be assimilated. The major fatty acids include C 14:0, C 16:0, C ω6 c, C 19:0 cyclo ω8 c, C 2-OH, summed feature 3 (C 16:1 ω6 c and/or C 16:1 ω7 c) and summed feature 8 (C 18:1 ω7 c and/or C 18:1 ω6 c). The predominant polar lipids include phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid, three unidentified aminolipids and two unidentified lipids.

17:1

14:0

The type strain, MEBiC09520 T (=KCCM 43320 T =MCCC 1 K03920 T), was isolated from a tidal sediment of Buksung- Po , Incheon, South Korea. The DNA G+C content of the type strain is 54.7 mol%. The GenBank/EMBL/MMBJ accession number for the 16S rRNA gene sequence is MK878394 and that of the genome sequence is CP040517 .