Longihyalospora ampeli Tennakoon, C.H Kuo & K.D. Hyde

Tennakoon, Danushka S., Thambugala, Kasun M., Jeewon, Rajesh, Hongsanan, Sinang, Kuo, Chang-Hsin & Hyde, Kevin D., 2019, Additions to Chaetothyriaceae (Chaetothyriales): Longihyalospora gen. nov. and Ceramothyrium longivolcaniforme, a new host record from decaying leaves of Ficus ampelas, MycoKeys 61, pp. 91-109 : 91

Longihyalospora ampeli Tennakoon, C.H Kuo & K.D. Hyde sp. nov. Figure 3

Etymology.

Species name based on the host Ficus ampelas , from which it was collected.

Holotype.

MFLU 19-0824

Description.

Epiphytic on the upper surface decaying leaves, appearing as small black dots. Covering the upper leaf surface with dark mycelium without penetrating host tissues. Mycelial pellicle (190-) 200-250 (-258) µm diam., elongate, subiculum-like, comprising hyphae that are mostly narrow, 1-2 μm wide (x- = 1.5 μm, n= 20), dense, dark brown. Mycelial setae (197-) 200-225 (-231) µm long, at base 10-12 µm wide, at apex 2-3 µm wide, dark brown, scattered, discrete, arranged as a ring around the pellicle, unbranched, formed on dense, dark hyphae. Sexual morph: Ascomata 55-90 μm high, 150-200 μm diam. (x- = 76 × 168 µm, n = 10) in diameter, superficial, solitary, dark brown to black, globose to subglobose, coriaceous, uni-locular, somewhat flattened when dry, covered by a mycelial pellicle. Peridium 18-25 μm wide (x- = 23.5 μm, n= 20), pale brown to brown, with compressed, hyaline, inner cells of textura angularis and dark brown outer cells of textura angularis. Asci (82-) 90-115 (-120) × 52-62 μm (x- = 106 × 57 µm, n = 20), 8-spored, bitunicate, broadly obovoid, slightly stalked, apically rounded, with well-developed ocular chamber. Ascospores (74-) 76 –98(– 105) × 10-12 μm (x- =84 × 10.8 µm, n = 30), overlapping, irregularly triseriate hyaline, elongate fusiform, (6-) 8-11 (-12) septa, slightly constricted at the middle septum, tapering to the ends, smooth-walled, surrounded by a 3.5-5 µm wide mucilaginous sheath. Asexual morph: Not observed.

Material examined.

Taiwan, Chiayi, Fanlu Township area, Dahu forest, decaying leaves of Ficus ampelas ( Moraceae ), 20 June 2018, D.S. Tennakoon, H50B1 (MFLU 19-0824, holotype), H50B2 (MFLU19-0825, isotype).

Notes.

Longihyalospora is described herein as a new monotypic genus in Chaetothyriaceae . Longihyalospora differs from other genera in Chaetothyriaceae by a combination of a dark mycelium covering the upper leaf surface, an elongate mycelial pellicle, ring of setae around the pellicle, pale brown to brown peridium with hyaline inner layers, broadly obovoid, short pedicellate asci and hyaline, elongate fusiform and 8-11-septate ascospores, with tapering ends and a thin mucilaginous sheath. In our phylogenetic analyses, Longihyalospora ampeli species constitutes a strongly supported sub clade, which is nested independently from other genera in Chaetothyriaceae ( Figure 1 ).

Figures

Figure 1. RAxML tree based on a combined dataset of ITS, LSU and SSU partial sequences of 45 taxa. Bootstrap support values for maximum likelihood (ML and, maximum parsimony (MP) values higher than 60 % and Bayesian posterior probabilities (BYPP) greater than 0.90 are given above each branch respectively. The new isolates are in red. Ex-type strains are in bold. The tree is rooted by Leptoxyphium fumago (CBS 123.26) and L. madagascariense (CBS 124766).

Figure 3. Longihyalospora ampeli (MFLU 19 - 0824, holotype). a Host leaf b appearance of colony (black spots) on leaf c ring of setae around the pellicle d mycelial pellicle with setae e mycelial pellicle cells f, g vertical section through ascoma h section of peridium i-m asci n-r ascospores s ascospore stained in Indian ink showing a mucilaginous sheath. Scale bars: 100 µm (c), 75 µm (d), 20 µm (e, f), 50 µm (g), 10 µm (h), 50 µm (i-m), 20 µm (n-s).