Kyrgyzstancampa sanare Sendra & Ferreira, 2021

Sendra, Alberto, Sánchez-García, Alba, Selfa, Jesús, Milko, Dmitry A. & Ferreira, Rodrigo Lopes, 2021, Campodeidae (Hexapoda: Diplura) from Kyrgyzstan, Central Asia, with the description of a remarkable new genus and species, European Journal of Taxonomy 782, pp. 1-20 : 5-13

publication ID	https://doi.org/ 10.5852/ejt.2021.782.1585
publication LSID	lsid:zoobank.org:pub:94DEA9B6-730C-49BC-B87F-54A4FA7F807C
DOI	https://doi.org/10.5281/zenodo.5762303
persistent identifier	https://treatment.plazi.org/id/012CDB29-579C-432F-B6C0-2DEE0F408BE5
taxon LSID	lsid:zoobank.org:act:012CDB29-579C-432F-B6C0-2DEE0F408BE5
treatment provided by	Felipe
scientific name	Kyrgyzstancampa sanare Sendra & Ferreira
status	gen. et sp. nov.

Treatment

Kyrgyzstancampa sanare Sendra & Ferreira gen. et sp. nov.

urn:lsid:zoobank.org:act:012CDB29-579C-432F-B6C0-2DEE0F408BE5

Figs 8–26 View Figs 2−9 View Figs 10−12 View Figs 13−15 View Figs 16−18 View Figs 19−24 View Figs 25−26

Etymology

The specific epithet is taken from the Latin ‘sanare’, meaning ‘cure’ and is related to the cave where the species was found, which is used for therapeutic purposes. This should be treated as a noun in apposition.

Type material

Holotype KYRGYZSTAN • ♀; “holotype- ♀ IBB 92101”; Ak-Turpak Cave ; 40º10′35.18″ N, 71º03′45.36″ E; alt. 900 m; 12 Aug. 2019; R.L. Ferreira leg.; IBB 92101. GoogleMaps

Paratypes KYRGYZSTAN • 1 ♀, mounted in Marc André II solution; “paratype- ♀ 01 MZB ( MCNB) 2021-2338”; same collection data as for holotype; MZB ( MCNB) 2021-2338 GoogleMaps • 1 ♀, mounted in Marc André II solution; “paratype- ♀ 02 Coll. AS”; same collection data as for preceding; Coll. AS GoogleMaps • 1 ♂, mounted in Marc André II solution; “paratype-♂1 MZB ( MCNB) 2021-2339”; same collection data as for preceding; MZB ( MCNB) 2021-2339 GoogleMaps • 1 ♂, mounted in Marc André II solution; “paratype-♂02 Coll. AS”; same collection data as for preceding; Coll. AS GoogleMaps .

Other material

Two specimens with the same data as the holotype were mounted on an aluminium stage and coated with palladium-gold.

Description

BODY. Length 3–3.9 mm (3–3.9 mm in females; 3.1 and 3.4 mm in males; 3.9 mm in holotype) ( Figs 8–9 View Figs 2−9 ). Epicuticle smooth under optical microscope on dorsal side of nota and legs, but, at high magnification, slightly reticulate with irregular polygonal structures of variable size ( Figs 16–17 View Figs 16−18 ). Body sparsely covered with short clothing setae bearing 0–3 tiny distal barbs.

HEAD. Two apparently intact antennae with 27–28 antennomeres; antennae 0.28–0.29 × length of body, with medial antennomeres 1.1× as long as wide; apical antennomere 1.9 × as long as wide. Cupuliform organ with about eight plain paddle-shaped olfactory chemoreceptor sensilla, 7 µm long ( Fig. 10 View Figs 10−12 ). Distal and medial antennomeres with two whorls of barbed macrosetae and scattered smooth setae, plus 2–4 short thin gouge sensilla 8–9 µm long ( Figs 11–12 View Figs 10−12 ). Proximal antennomeres with typical trichobothria, plus small bacilliform sensillum 6–7 µm long on 3 rd antennomere in ventral position ( Figs 13–14 View Figs 13−15 ). Plain frontal process with one anterior and three posterior smooth setae; length ratios of a / p 53/ 23 in holotype. Four short, smooth macrosetae along each side of antennomere insertion line with length ratios of a / i1 and i2 / p 11/15/14/ 11 in holotype; no x setae observed ( Fig. 15 View Figs 13−15 ). Small subtrapezoidal labial palp with small subcylindrical latero-external sensillum; two guard setae, up to three simple setae on anterior border, and up to 35 neuroglandular setae, as well as short and coniform palpiform sensillum, in holotype.

THORAX. Thoracic macrosetae distribution ( Figs 16–18 View Figs 16−18 ): pronotum and mesonotum with 1+1 ma, 1+1 la, 1+1 lp macrosetae; metanotum with 1+1 ma macrosetae. All macrosetae rather slender with short barbs along middle third; marginal setae similar to clothing setae ( Fig. 17 View Figs 16−18 ). Legs short, metathoracic legs reaching abdominal segment V, about 0.3 × length of body ( Fig. 19 View Figs 19−24 ). Large, deep joint between femur and tibia with longitudinal protrusion on inner side ( Fig. 20 View Figs 19−24 ). Femora I–III each with one middle-sized dorsal macroseta with few distal barbs, slightly longer than ventral macroseta. Calcars slightly thickened with long barbs on one side. Tibiae I‒III with one ventral macroseta with three or four distal barbs. Two rows of ventral barbed setae longer and thicker than clothing setae, with long thin barbs. Three smooth, distal dorsal tarsal setae longer than rest. Claws subequal, regularly curved, with tiny ventral and lateral microspines. Laminar lateral processes of pretarsus striated on dorsal side with ridges surpassing end of the apex, giving appearance of distal fringe, and with short barbs on ventral side ( Figs 21–24 View Figs 19−24 ).

ABDOMEN. Distribution of abdominal macrosetae on tergites: 1+1 lp on urotergite III; 1+1 la, 2+2 lp on urotergites IV–VIII; 3+3 lp on abdominal segment IX; 5+5 macrosetae on abdominal segment X; all macrosetae long, with thin barbs along distal half. Urosternite I apparently with 5+5 macrosetae ( Figs 25–26 View Figs 25−26 ); urosternites II–VII with 3+3 macrosetae; urosternite VIII with 1+1 macrosetae; longsized urosternal macrosetae with few distal barbs. Stylus with apical seta with two long basal teeth, subapical seta and ventromedial seta, each bearing a row of barbs along distal half, more abundant on ventromedial setae.

CERCI. 0.71 × length of body (on a cercus apparently intact in the holotype), with basal article divided into four secondary articles plus 11 primary articles; each primary article with central constriction bearing whorl of long macrosetae with thin barbs on distal part and one or two whorls of thin smooth setae; each primary article ending in whorl of thin setae, including apical article.

SECONDARY SEX CHARACTERS. Female urosternite I with short subcylindrical appendages, each bearing up to 11–13 glandular a 1 setae in distal field ( Fig. 27 View Figs 27−28 ). Male urosternite I with elongated subtrapezoidal appendages, each bearing up to 8 glandular a 1 setae in distal field and larger posterior field with up to 70 glandular a 2 setae; posterior edge of first urosternite with field of up to 44 glandular g 1 setae arranged in two rows ( Fig. 25 View Figs 25−26 ).

Type locality

Kyrgyzstan, Kadamjay District, Batken Region, Ak-Turpak Cave, gypsum cave located south of Ak-Turpak village; 40º10′35.18″ N, 71º03′45.36″ E.

Habitat

The specimens were observed only in the deep zone of Ak-Turpak Cave, located near the western margin of the Kadamjay District, Batken Province, Kyrgyzstan, which is located about 2.5 km south of the village of Ak-Turpak (northwestern part of Alai Mts.). The name of the locality means ʻwhite landʼ in the local Turkish dialect and reflects the prevalence of the whitish, pinkish, or reddish clayey ground surface. Its entrance is located about 400 m from the right bank of the river Sokh (Kozheshken) ( Fig. 2 View Figs 2−9 ), approximately 40–50 m a.s.l. The Sokh River divides the northern macroslopes of the Turkestan Mt Range and Alai (or Alay) Mt System. This area can also be considered as the southern edge of the Fergana Depression. The cave entrance is surrounded by a hilly relief, without any tops above 1000 m a.s.l. in a one-kilometre-neighbourhood. The landscape surrounding the Kyzyl-Unkuyr Cave is quite dry ( Figs 2−3 View Figs 2−9 ), with only sparse shrubby vegetation typical of rocky outcrops, where the soil is extremely shallow when present. On the other hand, the Sokh River floodplain, located quite close to the cave, is moist although it is currently very altered due to the presence of crops and small villages. However, suitable habitats for soil invertebrates certainly occur along this floodplain. It is worth mentioning that although Kyrgyzstancampa sanare Sendra & Ferreira gen. et sp. nov. was found in a cave, it does not show any troglomorphic morphological characters. Thus, it is likely that the species is not troglobitic, although further sampling in the external area surrounding the cave (especially along the floodplain of the Sokh River) is needed to confirm this hypothesis.

The Ak-Turpak cave has a single entrance, where a metallic structure was installed to safeguard and protect the cave’s entrance ( Figs 3−4 View Figs 2−9 ). From the entrance inwards, stairs were built to facilitate access for visitors. The cave gallery is comparatively simple and oriented east-northeast, with 137 m of linear extension and about 40 m deep ( Gvozdetskij 1981; Dudashvili & Mikhailyov 1990). The area of the cave was estimated to be 2400 m 2 and the volume is 8393 m 3 ( Mamatkulov 1978). The cave is situated in a gypsum stratum ( Gvozdetskij 1981) in the trough zone, where karstified rocks are represented by gypsum, marls, marlstones, limestones, and dolomites of Cretaceous and Paleogene ages ( Beloglazova & Smirnova 1987; Sultanov 1972). The origin of all karst forms in Southern Fergana is related to tectonic faults and sedimentary breccias, and they often developed as a result of repeated and sometimes overlapping karst processes ( Sultanov 1972).

In the upper part of the cave conduit, there is a noticeable proportion of soft marl that is somewhat dilapidated (during the last 5–10 years, this part of the gallery was equipped with a cement staircase and the walls were partly reinforced with rubble masonry panels to reduce dust and for balneological and recreational use). In the deeper parts of the gallery, the cave vaults are formed by fine-crystalline selenite (calcium sulphate dihydrate CaSO 4 •2H 2 O) of several, sometimes contrasting, colour shades. The north side of the cave is preferentially formed by argillite. The atmosphere of the Ak-Turpak cave is rather dry and there are no traces of thermokarst processes ( Dudashvili & Mikhailyov 1990); however, the cave vaults are somewhat crumbled after recent earthquakes.

Over the last decades, local residents (≈ 100–330 per year) have used the cave for therapeutic purposes (respiratory treatments: asthma, bronchitis, etc.) as word of mouth on the cave’s ʻhealing propertiesʼ has spread among them. Hence, one can see, especially in the entrance, small platforms and mattresses ( Fig. 5 View Figs 2−9 ). Visitors mostly use the entrance area, but stairs have also been installed deep inside the cave ( Fig. 6 View Figs 2−9 ), in which some mattresses were observed, indicating that the entire cave has been used for therapeutic purposes. Specimens of Kyrgyzstancampa sanare Sendra & Ferreira gen. et sp. nov. were only found in the last chamber of the cave, and always associated with old bat guano ( Fig. 7 View Figs 2−9 ). Several individuals were observed amidst the guano ( Figs 8−9 View Figs 2−9 ), rapidly escaping when disturbed. In these cases, they tended to enter the small spaces between the chitin fragments observed in the pile, so it was difficult to capture specimens without injuring them. The only organic resource observed inside the cave was bat guano from species of Rhinolophus Lacépède, 1799 (horseshoe bats) and a few organic materials left by visitors (such as cardboard pieces and wood). The cave is not well preserved as many accesses were built, thus deeply altering the pristine substrates. However, considering the lack of troglomorphic traits in the species (indicating that the cave is not its unique habitat) and given that apparently few visitors access the deeper parts of the cave, the species does not appear to be threatened.

Phyletic affinities

Kyrgyzstancampa Sendra & Ferreira gen. nov. has similarities with several species of the paraphyletic genus Eutrichocampa Silvestri, 1902 . In his diagnosis of Eutrichocampa , the tarsus ends abruptly instead of being acuminate towards the apex, which he considered to be a feature differentiating Eutrichocampa and Campodea ( Silvestri 1902) . For more than a century, several authors have been adding species to this genus, such as Wygodzinsky (1941, 1943), Condé (1947, 1994), Ionsecu (1955), Loksa (1960), García-Gómez (2016) and also Silvestri (1931a, 1932a, 1932b, 1933a), resulting in the current fifteen species of Eutrichocampa ( Sendra et al. 2021) . These species were described from localities scattered in the Americas, Africa, Asia, and Europe. In all of these contributions, the entire pretarsus shape is referred to as the differential character for Eutrichocampa : regularly curved claws with laminar or subcylindrical lateral processes with abundant barbs. Since Wygodzinsky (1941), Eutrichocampa has been considered a heterogeneous genus showing a wide variation in macrosetal patterns on the thorax and abdomen, including the presence or absence of dorsal macrosetae on the femora. In spite of the effort made by Condé (1956) to keep Eutrichocampa as a homogeneous taxon, several authors (including Condé himself) have tried to arrange it into several genera and subgenera ( Paclt 1957), proposing other genera with the same pretarsus trait and thoracic macrosetae of the Campodea pattern; for instance, Parallocampa Silvestri, 1933b with eleven species from North America, and Remycampa Condé, 1952 , with two species from northwest Africa and the Canary Islands, and four monotypic genera: Allocampa Silvestri, 1931b from Cuba; Edriocampa Silvestri, 1933a from the South Aegean islands and Anatolian Peninsula; Libanocampa Condé, 1955 from Lebanon and Anatolia; and Pseudolibanocampa Xie & Yang, 1991 from Guangdong and Yunnan in China. In 1957, Paclt proposed an artificial arrangement of Eutrichocampa by dividing it into four subgenera and the genus Leniwytsmania Paclt, 1957 for two species, both from China: L. orientalis ( Silvestri, 1931a) and L. helvetica ( Wygodzinsky, 1941) . Our proposal of Kyrgyzstancampa Sendra & Ferreira gen. nov. is another effort to unravel the diversity within the subfamily Campodeinae , in which this new genus can be included.

Several characters define Kyrgyzstancampa Sendra & Ferreira gen. nov., such as the pretarsus with a regularly curved claw with tiny ventral and lateral microspines; the laminar lateral processes, striated on the dorsal side with short barbs on the ventral side ( Figs 21–24 View Figs 19−24 ); a unique femur-tibia joint; the macroseta pattern on the nota: 3+3 ma, la, lp macrosetae on the pronotum and metanotum, 2+2 ma, lp on the metanotum plus one dorsal femoral macroseta; and 1+1 la and 2+2 lp on urotergites IV–VIII. Other notable features are the sparse clothing setae on the body, the plain paddle-shaped sensilla on the cupuliform organ, and the secondary sexual characters on the first urosternite. This combination of characters delineates Kyrgyzstancampa Sendra & Ferreira gen. nov. from other genera of Campodeinae and all species of Eutrichocampa . The closest species to K. sanare Sendra & Ferreira gen. et sp. nov. seems to be Eutrichocampa birabei Wygodzinsky, 1943 , described from San Antonio de Arredondo, Córdoba in Argentina. Both share the shape of the pretarsus, the distribution of macrosetae on the nota and urotergites, and a dorsal macroseta on the femora. However, K. sanare Sendra & Ferreira gen. et sp. nov. and E. birabei differ in the number of urotergal macrosetae and in the secondary sexual characters of the first urosternite. Furthermore, reuniting both species in Kyrgyzstancampa Sendra & Ferreira gen. nov. would be a far-fetched approach, and new material on the South American species will be necessary to provide a more accurate description.