Kondoa ribitophobia Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 105-106

Kondoa ribitophobia Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828798. Fig. 12O .

Etymology: the specific epithet ribitophobia refers to the physiological character of not assimilating ribitol.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are globosal, oval and ellipsoidal, 3.3– 4.9 × 4.5– 8.3 μm and single, budding is polar ( Fig.12O ),a sediment is formed.After1mo at17°C, a pellicle and a sediment are present. On YM agar, after 1 mo at 17°C,the streak culture is pale yellow,butyrous,smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (variable), L-sorbose (variable), sucrose, maltose, cellobiose (variable), trehalose, melezitose, inulin (variable), L-arabinose (variable), L-rhamnose (variable), ethanol (variable), glycerol, D-mannitol (delayed and weak), D-glucitol (variable), Methyl-α- D-glucoside (variable), salicin (weak) and succinate (variable) are assimilated as sole carbon sources. Lactose, melibiose, raffinose, soluble starch, D- xylose, D-arabinose, D-ribose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, erythritol, ribitol, galactitol, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), L-lysine (variable), ethylamine hydrochloride (variable) and cadaverine dihydrochloride (variable) are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 26–27 °C. Growth in vitamin-free medium is variable. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Kon. ribitophobia differs from its closely related species Kon. gutianensis in its inability to assimilate ribitol ( Table S1.17 ).

Typus: China, Taiwan province, obtained from a leaf of an unidentified plant, Aug. 2009, Q.-M. Wang (holotype CGMCC 2.4441 T preserved in a metabolically inactive state, ex-type CBS 12496 = TW2.1E-016).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.