Kirschsteiniothelia atra (Corda) D. Hawksw., Fungal Diversity

Tang, Xia, Jeewon, Rajesh, Jayawardena, Ruvishika S., Gomdola, Deecksha, Lu, Yong-Zhong, Xu, Rong-Ju, Alrefaei, Abdulwahed Fahad, Alotibi, Fatimah, Hyde, Kevin D. & Kang, Ji-Chuan, 2024, Additions to the genus Kirschsteiniothelia (Dothideomycetes); Three novel species and a new host record, based on morphology and phylogeny, MycoKeys 110, pp. 35-66 : 35-66

Kirschsteiniothelia atra (Corda) D. Hawksw., Fungal Diversity 69: 37 (2014)

Fig. 2

≡ Amphisphaeria aethiops Sacc., Syll. fung. (Abellini) 1: 722 (1882)

= Dendryphiopsis atra (Corda) S. Hughes, Can. J. Bot. 31: 655 (1953)

≡ Dendryphion atrum Corda, Icon. fung. (Prague) 4: 33 (1840)

≡ Kirschsteiniothelia aethiops (Sacc.) D. Hawksw., J. Linn. Soc., Bot. 91 (1–2): 185 (1985)

Description.

Saprobic on decaying wood of Edgeworthia chrysantha . Sexual morph: see Hawksworth (1985). Asexual morph: Colonies on the natural substrate superficial, effuse, gregarious, dark brown to black, glistening. Mycelium immersed, composed of branched, septate, thin-walled, smooth, brown hyphae. Conidiophores 253–396 × 8–15.5 µm (x ̄ = 334.6 × 11.7 µm, n = 20), macronematous, mononematous, erect, straight or flexuous, cylindrical, septate, smooth, brown to dark brown, becoming paler towards the apex and comprising numerous short branches. Conidiogenous cells 14.5–29 × 5–10 µm (x ̄ = 20.6 × 6.8 µm, n = 30), tretic, integrated, sometimes percurrent, terminal, doliiform or lageniform, subhyaline to pale brown, with new cells developing from the apical or subapical part of the subtending cells. Conidia 32–56.5 × 11–19.5 µm (x ̄ = 42.3 × 14.5 µm, n = 30), solitary, acrogenous, cylindrical, sometimes clavate, 3–4 - septate, constricted and darker at the septa, smooth, brown and rounded at the apex.

Culture characteristics.

Conidia germinating on Potato Dextrose Agar (PDA) within 24 h, and producing germ tubes either from the apex or base. Colonies circular, flat, dense, radial sulcate, edge entire, pearl-gray on the surface, dark brown on the reverse and becoming grey-white along the margin.

Material examined.

China • Guizhou Province, Zunyi City , Suiyang County, saprobic on decaying branches of Edgeworthia chrysantha , 13 February 2023, Xue-Mei Chen, SY 12 (GZAAS 23-0807), living culture GZCC 23-0731 .

Known distribution (based on molecular data).

China ( Su et al. 2016; this study).

Known hosts (based on molecular data).

Edgeworthia chrysantha (This study), Unidentified decaying wood ( Su et al. 2016).

Note.

Morphologically, our collection matches the characteristics of Kirschsteiniothelia atra , including macronematous, mononematous conidiophores with numerous short branches; tretic, doliiform, or lageniform conidiogenous cells that develop new cells from the apical or subapical part of the subtending cells; and cylindrical, occasionally clavate conidia that are 3–4 - septate, constricted and darker at the septa, which are rounded at the apex ( Su et al. 2016). In the phylogenetic analyses, our collection (GZCC 23-0731) clusters with Kirschsteiniothelia atra (CBS 109.53, DEN, MFLUCC 15-0424, MFLUCC 16-1104 and S – 783) (Fig. 1 ). Excluding gaps, no difference was observed in the comparison of nucleotides across the ITS (491 bp), LSU (788 bp) and SSU (844 bp) regions between our collection and Kirschsteiniothelia atra (MFLUCC 16-1104). Based on these findings, we identify our isolate as Kirschsteiniothelia atra , following the guidelines established by Jeewon and Hyde (2016) and Maharachchikumbura et al. (2021). This is the first time Kirschsteiniothelia atra has been reported from Edgeworthia chrysantha .

Figures

Figure 1. Phylogram of Kirschsteiniothelia taxa, based on the RAxML analysis of the combined ITS, LSU and SSU rDNA sequence dataset. Bootstrap support values for Maximum Likelihood (ML) equal to or greater than 75 % and Bayesian posterior probabilities (PP) equal to or greater than 0.95 are shown above the nodes. The tree is rooted with Strigula guangxiensis (HMAS- L 0138040) and S. nemathora (MPN 72). Newly-generated strains are denoted in red and type strains are indicated with a superscript “ T ”.

Figure 2. Kirschsteiniothelia atra (GZAAS 23-0807, new host record) a – c colonies on natural substrate d – g conidiophores and conidiogenous cells bearing conidia h – n conidia o a germinated conidium p upper surface view of culture q lower surface view of culture. Scale bars: 100 μm (d – g); 20 μm (h – o).