Hydnum khanspurense Niazi, Ghafoor, & M. Ali

Ali, Muhammad, Ghafoor, Aneeqa, Ghafoor, Annie, Niazi, Abdul Rehman & Swenie, Rachel A., 2024, Hydnum khanspurense sp. nov. and H. berkeleyanum from Pakistan, Phytotaxa 660 (2), pp. 161-170 : 163-166

Hydnum khanspurense Niazi, Ghafoor, & M. Ali sp. nov. ( Figures 2–3 )

MycoBank no. MB854208

Etymology:— The species epithet ‘ Hydnum khanspurense’ (Latin) refers to the type locality of the specimen.

Diagnosis:— This species is characterized by the fleshy, velutinous yellowish orange to pale orange plano-convex pileus, non-decurrent, conical light orange spines, central stipe, smaller broadly ellipsoid to obovoid basidiospores (7.02–8.87 × 5.51–8.03 µm) and basidia (32.31–49.13 × 5.55–8.61 µm) with 1–4 sterigmata.

Holotype:— Pakistan, Khyber Pakhtunkhwa, Khanspur , 2250 m a.s.l., under Pinus wallichiana A.B. Jacks. , solitary or gregarious on decomposing needles and soil, 08 August 2021, Abdul Rehman Niazi & Aneeqa Ghafoor, KH-09 ( LAH88021 ). GenBank accession no: OQ130694.

Basidiomes 22–61 mm high, fleshy. Pileus 20–42 × 18–38 mm, Surface velutinous yellowish orange (8/8 YR 10) to pale orange (8/3 YR 5), smooth, plano-convex, slightly depressed from center. Margin entire, straight, yellowish orange (8/8 YR 10). Context 2–4 mm wide, yellowish white. Spines 1.3–1.9 × 0.2–0.3 mm, conical, acute, light orange, crowded, slightly adnexed. Stipe 20–58 × 8–14 mm, white, smooth, central, cylindrical, slightly broader at the base and covered with white colored mycelium. Odour fruity.

Basidiospores [40/3/3] (7.02) 7.12–8.74(8.87) × (5.51) 5.66–7.69 (8.03) µm, Qavl × Qavw = 7.86 × 6.65 µm, Q = 1.18, broadly ellipsoid to obovoid, thin walled, hyaline, smooth, with granular contents. Basidia 32.31–49.13 × 5.55– 8.61 µm, clavate to sometime narrowly clavate, clamps present at the base, with 1–4 sterigmata. Sterigmata 2.63–5.67 × 0.97–2.26 µm, Basidioles 21.66–47.11 × 4.26–13.37 µm, numerous clavate to subclavate, Hyphae present at apex of spines 4.53–6.97 µm wide, cylindrical, thin-walled, clamped. Pileipellis a cutis composed of hyphae 5.8– 10.2 µm wide, interwoven, cylindrical, clamped. Stipitipellis composed of hyphae 3.96–11.18 µm wide, interwoven, cylindrical, clamped.

Habit and Habitat:— solitary to gregarious, growing on soil and decomposing needles of pine trees in pinedominated moist temperate forest of Northern Pakistan.

Additional material Examined: Pakistan, Khyber Pakhtunkhwa, Helipad forest near Khanspur village, 2250 m a.s.l., under Pinus wallichiana , solitary and gregarious on soil and decomposing needles, 29 July 2022, Abdul Rehman Niazi and Aneeqa Ghafoor, KH 50 ( LAH 29722). GenBank accession no: OQ130695. Pakistan, Khyber Pakhtunkhwa, Abbottabad district, Kuza Gali, 2452 m a.s.l., under Pinus wallichiana , 20 August 2022 Abdul Rehman Niazi and Aneeqa Ghafoor, KH-451 ( LAH 20822), GenBank accession number: OQ130696

Hydnum berkeleyanum K. Das, Hembrom, A. Baghela & Vizzini in Wang, Das, Horman, Antonin, Baghela, Chakraborty, Hembrom, Nakasone, Ortiz-Santana, Vizzini, Hofstetter & Buyck, Cryptog. Mycol. 39(2): 212 (2018) ( Figures 2–3 )

Pileus 20–55 mm diam., convex or planoconvex to plane, centrally depressed or umbilicate, surface dry, shiny, hygrophanous, small scales rarely present at the margin; orange-brown (5 YR 5/6) to bright reddish orange (10 R 5/12), or pale orange (9.5 R 5/12) towards the margin. Margin entire, slightly incurved turning straight to lobed or undulate with age. Context 2–4 mm wide, white, yellowing at the cut. Spine 3–5 × 0.4–0.7mm adnate, conical, acute, crowded, white to pale orange. Stipe 12–70 × 5–15 mm, central to eccentric, cylindrical or clavate, surface tomentose-pruinose, whitish, turning yellow on bruising or handling.

Basidiospores [30/2/2] (6.5–) 7.0–8.5(–9) × (6.0–) 6.5–7.5 (–8.0) µm, Qavl × Qavw = 7.84 × 6.98 µm Q =1.07, globose or subglobose, to ovoid, thin-walled, non-amyloid, hyaline, apiculate, Basidia 30–48.5 × 7.0–9.5 µm, clavate, 2–4 spored, with granular content, frequently clamped, Hyphae present at apex of spines 3.1–5.5 µm wide, cylindrical, thin-walled, Pileipellis a cutis of cylindrical, tightly interwoven, thin-walled hyphae 2.6–10 µm wide, clamped, with round to slightly tapering ends, Stipitipellis composed of cylindrical, parallel, thin walled, clamped hyphae, 3.0–5.0 µm broad with round ends.

Habit and Habitat:— gregarious under Pinus wallichiana in mixed forests of Pakistan and temperate broadleaf forests of India; also China.

Additional material examined:— Pakistan, Khyber Pakhtunkhwa, Abbottabad district, Ayubia National Park, 2400 m a.s.1, 12 August, 2017, Muhammad Ali and Abdul Rehman Niazi, KH 373 ( LAH 12817) GenBank accession no: OQ130700. ibid. 08 August 2018, Abdul Rehman Niazi and Muhammad Ali, KH 421 ( LAH 08818), GenBank accession number: OQ130701.

Figures

FIGURE 2. Macromorphology of basidiomata of Hydnum khanspurense (A–B, LAH88021–LAH29722), and H. berkeleyanum (C–D, LAH12817- LAH08818). Scale bar (A–D) = 1 cm.

FIGURE 3. Line Drawings of Hydnum khanspurense (A–D, LAH88021, LAH29722, LAH20822) and H. berkeleyanum (E–G, LAH12817–LAH08818).A–B. Basidia and basidioles; C. Basidiospores; D. Pileipellis; E. Basidia; F. Basidiospores; G. Pileipellis (Scale bar A–F=5μm).