Fusarium lumajangense N. Maryani, Sand.

Maryani, N., Sandoval-Denis, M., Lombard, L., Crous, P. W. & Kema, G. H. J., 2019, New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia, Persoonia 43, pp. 48-69 : 59

Fusarium lumajangense N. Maryani, Sand. -Den., L. Lombard, Kema & Crous, sp. nov. — MycoBank MB828960; Fig. 6

Etymology. Name refers to Lumajang, the region from where this species was collected in Indonesia.

Typus. INDONESIA, Desa Kandang Kepus, Kecamatan Senduro,Lumajang , East Java (E113°4'157" S8°4'46"), in infected pseudostem of Musa acuminata var. Pisang Mas Kirana ( AA), 17 July 2014, N. Maryani (holotype specimen and culture, InaCC F 872 , preserved in metabolically inactive state).

Sporulation abundant from conidiophores carried on aerial mycelium and from sporodochia. Conidiophores on aerial mycelium, septate, branching profusely, irregularly or sympodially or reduced to solitary conidiogenous cells formed laterally on aerial hyphae; conidiogenous cells mono- or polyphialidic, acute, subulate or subcylindrical, smooth- and thin-walled (6–)10–22.5(–31.5) × 2–3(–4) µm, formed terminally and singly on conidiophores or intercalary, often proliferating percurrently; periclinal thickening inconspicuous or absent; conidia of two types: a) (microconidia) ovoid to ellipsoid, smooth- and thin-walled, (6–)9–18(–23) × (2–)3(–5) μm (av. 13 × 4 µm), 0–1-septate, arranged in false heads on monophialides; and b) (macroconidia) falcate and multiseptate, apical cells papillate, basal cells indistinct or foot-shaped, (1–2–)3-septate, formed on polyphialides; 1-septate conidia 18.5 × 3.5 µm; 2-septate conidia 40 × 4 µm; 3-septate conidia (26–)29–39.5(–44.5) × (3 –)3.5 – 4.5(– 5.5) µm; av. (18.5 –)28 – 39.5(– 44.5) × (3–)3.5–4.5(–5.5) µm. Sporodochia formed abundantly on surface of carnation leaves after 7 d, pale orange to orange. Conidiophores on sporodochia, septate, mostly unbranched or rarely sparsely and irregularly branched, bearing terminal monophialides, carried singly or grouped in verticillately branched; conidiogenous cells monophialidic, ampulliform, doliiform to subcylindrical, smooth- and thin-walled, (11.5–)12.5–18.5(–23.5) × (2–)3–4(–4.5) µm, proliferating percurrently several times, with short collarets and inconspicuous periclinal thickening; sporodochial conidia falcate, apical cells gently curved, papillate, basal cells slightly curved, foot-shaped, 3–5-septate: 3-sep- tate conidia, (30–)34.5–46.5(–54) × 3.5–4.5 µm; 4-septate conidia, 41–48(–52.5) × (3–) 3.5–4.5 µm; 5-septate conidia, (42.5–)45–53(–56) × 3.5–4.5 µm; av. (30–)40–50.5(–56) × (3–)3.5–4(–4.5) µm. Chlamydospores not observed.

Culture characteristics — Colony on PDA showing optimal growth at 25 °C with an average growth rate of 3.5–4.6 mm /d. Colony reverse, lilac to violet becoming white towards the margin, later becoming dark purple with time. Colony surface dry, white becoming livid purple towards the margin, turning completely purple with age.Aerial mycelium abundant, cottony, with moderate sporulation and lacking exudates.

Geography & Host — Lumajang, East Java, Musa acuminata . var. Pisang Mas Kirana (AA).

Pathogenicity — Non-pathogenic on Cavendish (AAA).

Additional material examined. INDONESIA, Desa Kandang Kepus, Ke- camatan Senduro, Lumajang , East Java (E113°4'157" S8°4'46"), in infected

pseudostem of Musa acuminata var.Pisang Mas Kirana ( AA), 17 July 2014, N. Maryani (InaCC F 993).

Notes — Fusarium lumajangense exhibits similar morphological features to F. mangiferae ( Britz et al. 2002) , also clus- tering in a sister relationship with the latter species. However, besides its clear phylogenetic delimitation, the polyphialides found in F. lumajangense commonly present two conidiogenous loci.

Figures

Fig. 6 Fusarium lumajangense (ex-type InaCC F993). a. Culture grown on PDA; b–c. sporodochia on carnation leaves; d–i. aerial conidiophores and phi- alides; j–m. aerial conidia; n–p. sporodochial conidiophores and phialides; q–s. sporodochial conidia. — Scale bars: b–d = 50 µm; e = 5 µm; f–s = 10 µm.