Filobasidium mucilaginum Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 103

Filobasidium mucilaginum Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828790. Fig. 12A .

Etymology: the specific epithet mucilaginum refers to the mucoid colony morphology of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are subglobosal and ellipsoidal, 3.8 –8.1 × 3.8– 8.8 μm and single, budding is polar ( Fig. 12A ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is gray-cream, mucoid, smooth and shiny. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose (delayed and weak), melibiose (delayed and weak), raffinose, melezitose, soluble starch (weak), D-xylose, L-arabinose (delayed and weak), D-arabinose (delayed and weak), ethanol (delayed and weak), glycerol (delayed and weak), erythritol (delayed and weak), ribitol (delayed and weak), galactitol, D-mannitol, D-glucitol, Methylα- D-glucoside, salicin, succinate (delayed and weak) and myo-inositol (delayed and weak) are assimilated as sole carbon sources. L-sorbose, inulin, D-ribose, L-rhamnose, D-glucosamine, methanol, DL-lactate, citrate and hexadecane are not assimilated. Ammonium sulfate and potassium nitrate (delayed and weak) are assimilated as sole nitrogen sources. Sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/ w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Fi. mucilaginum differs from its closely related species Fi. globosum and Fi. mali in its inability to assimilate Methyl-α- D-glucoside and its ability to assimilate L-sorbose and D-glucitol ( Table S1.15 ).

Typus: China, Sanya county, Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3463 T preserved in a metabolically inactive state, ex-type CBS 15486 = SY2.1).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.