Filobasidium globosum Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 100

Filobasidium globosum Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828788. Fig. 10O .

Etymology: the specific epithet globosum refers to the globosal vegetative cells of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are globosal, 2.7–6.7 × 2.7–6.7 μm and single, budding is polar ( Fig. 10O ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is gray-cream, mucoid, smooth and shiny. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not

observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin, D-xylose (delayed and weak), L-arabinose, L-rhamnose (delayed and weak), D-mannitol, Methylα- D-glucoside (weak), succinate (weak) and myo-inositol (weak) are assimilated as sole carbon sources. L-sorbose, soluble starch, D-arabinose, D-ribose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, D-glucitol, salicin, DL-lactate, citrate and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Fi. globosum differs from its closely related species Fi. mali in its inability to assimilate ribitol, galactitol, salicin and ethylamine and its ability to assimilate lactose and grow in vitamin-free medium ( Table S1.15 ).

Typus: China, Yichun county, Heilongjiang province, obtained from a leaf of an unidentified plant, Aug. 2014, Q.-M. Wang (holotype CGMCC 2.5680 T preserved in a metabolically inactive state, ex-type CBS 15658 = HLJ8A3).

Figures

Fig. 10. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) De. melastomatis CGMCC 2.3459T; (C, D) De. taiwanicus CGMCC 2.4429T; (E, F) De. ovatus CGMCC 2.3572T; (G, H) De. longicylindricus CGMCC 2.5660T;; (I) Pha. lactea CGMCC 2.5810T; (J) Pha. ovata CGMCC 2.5614T; (K, L) Ho. saccardoi CGMCC 2.3445T; (M) So. gelidoterrea CGMCC 2.5814T; (N) Fi. dingjieense CGMCC 2.5649T, (O) Fi. globosum CGMCC 2.5680T; (P) Fi. mali CGMCC 2.4012T. Bars = 10 μm.