Eprius (Eprius) veledinus Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 72-73

Eprius (Eprius) veledinus Grishin , new species

https://zoobank.org/ C135FD94-6DC9-4C82-9C2E-8A62D34DA640

( Fig. 7 part, 175–176, 407–409)

Definition and diagnosis. Phylogenetic trees reveal that a specimen from Ecuador identified as Eprius veleda (Godman, 1901) (type locality in Mexico: Veracruz and Tabasco, Guatemala, Honduras, and Panama, syntypes sequenced as NVG-21014A08 and NVG-21014A09) shows prominent genetic differentiation from it ( Fig. 7): e.g., their COI barcodes differ by 2.7% (18 bp), and therefore represents a new species. This new species keys to “ Epeus veleda veleda ”(J.5.(a)) in Evans (1955) but differs from the true E. veleda by less extensive white overscaling on the abdomen beneath, which is present in the form of two stripes rather than the entire ventral side white with a narrow dark stripe in the middle ( Fig. 176) and harpe more expanded dorsad into a small lobe ( Fig. 409). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly276665.5.1:A864G, aly276665.5.1:A912G, aly 2790.11.3:T489G, aly2487.46.1:T39G, aly2487.46.1:T45C, aly770.26.3:A73A (not C), aly860.3.1:T690T (not C), aly860.3.1:C723C (not T), aly3241.2.5:G289G (not A), aly86.14.6:C90C (not T), and COI barcode: T25C, G34C, T100G, T479C, T583C.

Barcode sequence of the holotype. Sample NVG-8019, GenBank OR837702, 658 base pairs: AACCTTATATTTTATTTTTGGTATCTGAGCTGGCATACTAGGAACATCCTTAAGATTATTAATTCGTACAGAATTAGGTAATCCTGGATCTTTAATT GGGGATGATCAAATTTATAATACTATTGTAACAGCTCACGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGTTTTGGTAATT GATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCTTTCCCCCGAATAAATAATATAAGATTTTGGATACTACCACCTTCTTTAATATTATTAAT CTCAAGAAGAATTGTTGAAAATGGTGCTGGAACTGGATGAACTGTTTATCCCCCCCTTTCATCTAATATTGCTCACCAAGGTTCTTCTGTAGATTTA GCAATTTTTTCTTTACATTTAGCAGGAATTTCTTCTATTTTAGGAGCTATTAATTTTATTACTACAATTATTAATATACGAATTAAAAATCTATCAT TTGATCAAATACCTTTATTTGTTTGATCTGTAGGTATTACAGCTTTATTGTTATTATTATCTTTACCTGTATTAGCTGGGGCTATTACAATATTACT CACTGATCGAAATTTAAATACTTCATTTTTTGACCCAGCAGGAGGAGGAGACCCAATTTTATATCAACACTTATTT

Type material. Holotype: ♂ deposited in the National Museum of Natural History, Smithsonian Institution , Washington, DC, USA ( USNM), illustrated in Fig. 175–176, bears the following five rectangular labels, four white: [ ECUADOR Pichincha | Alluriquin 700m | 26 May ’88 | S. S. Nicolay], [DNA sample ID: | NVG-8019 | c/o Nick V. Grishin], [genitalia | NVG170208-04 | Nick V. Grishin], [USNMENT | {QR Code} | 01321859], and one red [HOLOTYPE ♂ | Eprius (Eprius) | veledinus Grishin ].

Type locality. Ecuador: Pichincha Province, Alluriquin, elevation 700 m.

Etymology. The name is formed from the name of its northern sister species ( E. veleda ), which is made longer to indicate southern origin. The name is an adjective.

Distribution. Currently known only from the holotype collected in Ecuador.