Entomobrya uambae Arlé, 1959

Viana, Stéphanie Dos Santos, Morais, José Wellington De & Cipola, Nikolas Gioia, 2024, Taxonomic revision of Entomobrya Rondani, 1861 (Collembola: Entomobryidae: Entomobryinae) from the Brazilian Amazon, Zootaxa 5452 (1), pp. 1-110 : 41-51

Entomobrya uambae Arlé, 1959

Figs 29–35 , Table 2

Entomobrya uambae Arlé, 1959: 170 , figs 136–142, Brazil, Mato Grosso state, Alto Xingu municipality [currently Park of “Xingu”] in “Camaiurá” hamlet [currently “Kamayurá”] and “Pôsto Indigena Capitão Vasconcelos” [currently “Posto Leonardo Villas Bôas”] (original description).

Typological note. The type material of E. uambae deposited at MNRJ was destroyed in the fire of September 2018. The neotype is herein designated from specimens collected near to type locality, Park of “Xingu” ( Fig. 35 ) (ICZN 2000, chapter 16, article 75.3.4 and 75.3.6., and article 76). The specimens fit all morphological characteristics presented in the original description ( Arlé, 1959).

Examined type material. Neotype male designed on slide ( INPA- CLL 0000269 ): Brazil, Mato Grosso state, Feliz Natal municipality, “Aliança” farm, about 50 km from the Park of “Xingu”, 12°08’47”S, 54°29’30”W ( Fig. 35 ), 364 m, 10–15.vi.2022, pitfall-trap, NG Cipola & SS Viana coll.

Other examined material. Nine males and 2 females on slides and 15 specimens in alcohol ( INPA): same date as neotype . Nine specimens, 3 specimens in alcohol each institution ( CC / UFRN, MNRJ, MZUSP): same date as neotype . Two females on slides and 3 specimens in alcohol ( INPA): Brazil, Mato Grosso state, Feliz Natal municipality, forest fragment to 67 km from the Park of “Xingu”, 12°10’55”S, 54°36’34”W, 386 m, 10–15.vi.2022, pitfall-trap, NG Cipola & SS Viana coll. Two females on slides and 13 specimens in alcohol ( INPA): idem, except 12°21’15”S, 54°48’13”W , 360 m. Fifteen specimens in alcohol ( INPA): idem, except 12°21’03”S, 54°50’11”W, 278 m . One male on slides and 2 specimens in alcohol ( INPA): Novo Mundo municipality, State Park of “Cristalino”, 09°28’39.2”S, 55°51’37.6”W, 278 m, v.2013, pitfall-trap, RE Vicente coll. One male on slide ( INPA): idem, except 09°28’29.2”S, 55°50’00.2”W, 302 m, xi.2012 . One specimen in alcohol ( INPA): idem, except 09°36’36”S, 55°20’26”W, 428 m, 18.iv.2021, DJ Rodrigues coll. One female on slide and 1 specimen in alcohol ( INPA): Brazil, Mato Grosso, Vera municipality, forest fragment in margin to “MT-225” road, 12°15’26”S, 55°14’00”W, 390 m, 10–15.vi.2022, pitfall-trap, NG Cipola & SS Viana coll. Two males on slides and 1 specimen in alcohol ( ABAM / UFMT): Santa Cruz do Xingu municipality, State Park of “Xingu”, 09°51’17.97”S, 52°30’21.62”W, 268 m, 8–10.ii.2021, pitfall-trap, I Fagner & M Penhavek coll. One female on slide and 1 specimen in alcohol ( INPA): Vila Bela da Santíssima Trindade municipality, margin of “Guaporé” river, 15°01’15.9”S, 59°57’58.4”W, 203 m, 9–10.ix.2012, pitfall-trap, JMC Nascimento & N Hamada coll. One female on slide ( INPA): idem, except Campos de Julho municipality, margin of “Juína” river, 13°47’41”S, 59°27’16”W, 481 m, 7–8.ix.2012 . One male on slide ( INPA): idem, except Sapezal municipality, “Papagaio” river, 13°33’39.5”S, 58°24’24.2”W, 437 m, 01–02.xi.2012 . One female and 1 male on slide and 5 specimens in alcohol ( INPA): Sinop municipality, forest fragment in south of “UFMT”, 11°52’02”S, 55°28’59”W, 380 m, 7–9.vi.2022, pitfall-trap, NG Cipola & SS Viana coll. One juvenile on slide and 1 specimen in alcohol ( ABAM / UFMT): idem, except 25.ii.2017, winkler-trap, GER Santos & K Solera coll. One female on slide and 16 specimens in alcohol ( ABAM / UFMT): idem, except 02–04.viii.2017, pitfall-trap . One specimen in alcohol ( INPA): idem, except Forest Park of “Sinop”, 11°50’23”S, 55°30’02”W, 375 m, 9–13.vi.2022, yellow tray-trap, NG Cipola & SS Viana coll. One male on slide and 7 specimens in alcohol ( ABAM / UFMT): idem, except 11°50’14.22”S, 55°30’2.65”W, 381 m, 19–20.vii.2017, pitfall-trap, GER Santos & K Solera coll. One female on slide and 1 specimen in alcohol ( ABAM / UFMT): idem, except Botanical Garden Park of “Sinop”, 11°52’25.54”S, 55°30’32.00”W, 381 m, 21.vii.2017, winker-trap . One female on slide and 4 specimens in alcohol ( ABAM / UFMT): idem, except forest fragment of “ Marakai ” farm, 11°35’01.61”S, 55°19’13.60”W, 374 m, 9–10.vii.2017, pitfall-trap . One female on slide and 14 specimens in alcohol ( ABAM / UFMT): idem, except forest fragment in MT-140 road, near to “ Branca de Neve ” community, 11°50’40.91”S, 55°22’18.1”W, 391 m, 10–11.vii.2017 .

Diagnosis. Body yellowish, with a transverse band of dark pigment on Th III–Abd III, and another on Abd IV central region, Abd IV and femur III always blackish distally ( Fig. 29 ) Ant IV without the apical bulb ( Fig. 30A ); pl1 chaetae smooth and bifurcated ( Fig. 30D ); head macrochaetotaxy with 7–9 ‘ An ’, 3 ‘ A ’, 4–5 ‘ M ’, 8–9 ‘ S ’, 1 ‘ Ps ’, 4 ‘ Pa ’, 2 ‘ Pi ’, 2 ‘ Pm ’, 1–3 ‘ Pmp ’, 4 ‘ Pp ’, 1 ‘ Ppe ’ and 3 ‘ Pe ’ mac, respectively ( Fig. 30E ); labral papillae with 2 projections ( Fig. 31A ); labial papilla E l.p. conical, reaching or surpassing the base of a.a. ( Fig. 31B ); sublobal plate with 3 inner appendages ( Fig. 31C ); Th II internally with 8–9 anterior, 4–9 median and 17–20 posterior mac; Th III–Abd I with 18–21 and 6–11 mac, respectively; Abd II–III with 3–4(5) and 0 inner mac, respectively; Abd IV with 20–28 inner and 12–16 outer mac ( Figs 32A–C ); unguiculus III ai lamella slightly truncate distally and pe smooth ( Fig. 33G ); collophore anteriorly with 6 distal mac ( Fig. 34A ); dens distally with 1 inner chaeta larger than the others and modified; mucro proximal tooth larger than the distal one, basal spine surpassing the apex of the proximal tooth ( Fig. 34D ).

Redescription. Total length (head + trunk) of specimens 1.78–2.32 mm (n=4), neotype 2.06 mm. Specimens yellowish with violet to blackish pigments on distal half or entire Ant IV, distal half of Ant III, proximal and distal regions of Ant II, Ant I basally pigmented or not; Th II–Abd III with a transverse band heavily or weakly pigmented; Abd IV central region with an irregular transverse band, rarely absent; Abd IV and femur III (sometimes I–II) always black distally; head, Th II, coxa I–III (sometimes strongly) and femur I–III depigmented or weakly pigmented; collophore, tibiotarsus I–III, manubrium and dens yellowish or whitish; eyepatches black ( Fig. 29 ).

Head. Antenna subequal or longer than the trunk length ( Figs 29G–L ), ratio antennae: trunk = 1.25–0.91: 1 (n=4), neotype 1.19: 1; antennal ratio as I: II: III: IV = 1: 1.27–1.48: 1.14–1.30: 1.72–2.53 (n=4), neotype 1: 1.35: missing: 2.43. Ant IV weakly annulated on its distal 2/3 and devoid of apical bulb, with 4 apical finger-shaped sens, and numerous sens of different sizes ( Fig. 30A ). Ant III sense organ with 2 leaf-shaped sens, 3 guard sens, 1 elongated sens and at least 7 sens of different sizes ( Fig. 30B ). Ant II dorsally with about 5 elongated sens and 4 mac, without ventral mac ( Fig. 30C ). Ant I dorsally with 5–7 mac and 4–5 smooth mic at the base ( Fig. 30C ). Eyes A, B and C larger, G and H slightly smaller than the others, and with 6 interocular mes (q, v, s, p, r, t); head dorsal chaetotaxy ( Fig. 30E ) with 7–9 ‘ An ’, 3 ‘ A ’, 4–5 ‘ M ’, 8–9 ‘ S ’, 1 ‘ Ps ’, 4 ‘ Pa ’, 2 ‘ Pi ’, 2 ‘ Pm ’, 1–3 ‘ Pmp ’, 4 ‘ Pp ’, 1 ‘ Ppe ’ and 3 ‘ Pe ’ mac, respectively. Clypeal formula with 4 (l1–2), 12–16 (f), 3–11 (pf0–5) ciliate chaetae, l1–2 longer and apically acuminate, 1–3 f subtly larger, others subequal ( Fig. 30D ). Prelabral chaetae smooth, pl1 equally or subequally bifurcated, pl2 normal or bifurcated, equally or unequally ( Fig. 30D ). Labral papillae with 2 projections, inner papillae subtly larger than the outer papillae ( Fig. 31A ). Labial papilla E l.p. conical and reaching or surpassing the base of a.a. ( Fig. 31B ). Maxillary palp b.c. 1.24 longer than t.a.; sublobal plate with 3 inner appendages (sb1–3) ( Fig. 31C ). Basolateral and basomedian labial fields with M1–2, R, E, L1–2 ciliate, M2 subtly smaller or subequal to the others, present or absent ( Fig. 31D ). Cephalic groove with 9 ciliate chaetae of different length; postlabial formula with 4 (G1–4), 4 (X2–4), 4 (H1–4) chaetae, plus 1 chaeta between the series ( Fig. 31E ).

Thorax chaetotaxy ( Fig. 32 ). Th II a, m and p series (excluding the anterior collar) with 8–9, 4–9 and 17–20 mac, respectively. Th III a, m and p series with 7, 2–3 and 9–11 mac, respectively. Ratio Th II: III = 1.84–1.39: 1 (n=4), neotype 1.84: 1.

Abdomen chaetotaxy ( Figs 32B–C ). Abd I a, m and p series with 1–2, 5–7 and 0–2 mac, respectively. Abd II a, m and p series with 0–2 (1 mac rarely present), 4–5 and 0–1 mac, respectively. Abd III a, m and p series with 0–1, 2–3 and 1 mac, respectively.Abd IV with 20–28 (0–2 unpaired and 1 extra mac) inner mac of A–C series and 13–16 outer mac of T–Fe series; about 18 sens (ps type I and others type II) and 16 posterior mes. Abd V a, m, pa and p series with 1, 3, 1 and 5 mac, respectively. Ratio Abd III: IV = 1: 5.61–7.00 (n=4), neotype 1: 6.42.

Legs ( Fig. 33 ). Subcoxa I with 2 chaetae and 2 psp; subcoxa II with an anterior row of 7 chaetae and about 9 anterior chaetae; posterior row of 4 chaetae and 4 psp; subcoxa III with one row of 8 chaetae, about 19 anterior chaetae and 2 psp ( Figs 33A–C ). Trochanteral organ with 28–39 spine-like chaetae ( Fig. 33D ). Tibiotarsus I–III with 1 anterior and 7–9 inner finely ciliate mac; 1 outer apically acuminate mac at the base ( Fig. 33E ). Unguis b.t. on proximal 2/3, m.t. on distal 1/3 and smaller than b.t., a.t. minute and on distal 1/7. Unguiculus I–III lamellae (ae, ai, pe, pi) smooth and acuminate, except II–III with ai slightly truncate distally and II pe serrated with a small proximal tooth; ratio unguis: unguiculus = 1: 0.58. Tibiotarsus III smooth chaeta 1.31 longer than the unguiculus; tenent hairs 0.94 shorter than the unguis ( Figs 33F–G ).

Collophore ( Fig. 34A ). Anterior side with about 31 ciliate chaetae, 6 distal mac and about 25 chaetae of different sizes widely distributed on the corpus; posterior side with about 90 ciliate chaetae of different sizes widely distributed on the corpus (9 unpaired) and distally with 1 smooth chaeta; lateral flap with 14–16 chaetae, 9–14 smooth and 3–5 ciliate.

Furcula ( Figs 34B–D ). Manubrium ventrally with 2 subapical and about 39 distal ciliate chaetae of different sizes. Manubrial plate dorsally with 7–9 chaetae of different sizes and 3 psp. Dens dental lobe with 2 (bs1 and bs2) thicker ciliate chaetae, bs1 subequal to bs2. Dens tightly crenulate on its distal ¼ distally with 1 inner chaeta larger than the others and modified. Mucro proximal tooth larger than the distal one, mucronal spine surpassing the proximal tooth apex.

Variations. Specimens of E. uambae from different localities mostly show similar variations, except one specimen from Campos de Julho municipality which has a3 mac on both sides of the Abd II (generally absent) ( Fig. 32B ), and the whitish body with purple pigments, but with the same spots pattern ( Figs 29F and 29L ). Considering that only these two differences were observed, we believe there are only small variations in the populations.

Distribution. In the original description, E. uambae was reported only for its type locality (Mato Grosso state, Alto Xingu municipality). Later, the species was also recorded in the Amazonas state ( Oliveira & Deharveng 1995), but we are herein invalidating this Amazonas record for two reasons. The first is that the Amazonas specimens were incorrectly identified as E. uambae , and instead they belong to Amazhomidia thaisae Cipola & Bellini, 2018 (see Cipola et al. 2018b: 411). This mistake was made likely due to the similarity in the coloration patterns (yellowish with a dark transverse stripe on Abd IV posteriorly) ( Fig. 29 ) between both species (Oliveira, E.P. personal communication). The second is that after extensive sampling in the present study, E. uambae was only found in Mato Grosso state ( Fig. 35 ).

Remarks. Entomobrya uambae resembles E. egleri (see the remarks for E. egleri and Table 2).

New species of Entomobrya from the Brazilian Amazon

Figures

FIGURE 29. Entomobrya uambae habitus of fixed specimens in ethanol from Mato Grosso state: (A–F, dorsal view; G–L, lateral view); A–C and G–I respectively, specimens from Feliz Natal municipality; D and J, specimens from Vera municipality; E and K, specimens from Vila Bela da Santíssima Trindade municipality; F and L, specimens from Campos de Julho municipality. Scale bars: 1 mm, except E and K (0.5 mm).

FIGURE 30. Entomobrya uambae antennae and dorsal head: A, apex of left Ant IV (dorsal view); B, Ant III distally (lateral view); C, chaetotaxy of left Ant II–I (dorsal view) respectively, arrows indicate elongated sens, dorsal apically foot-shaped mac, and smooth mic present or absent in Ant I; D, clypeal, prelabral and labral chaetotaxy, arrow shows pl2 chaeta bifurcated or normal; E, head chaetotaxy (left side).

FIGURE 31. Entomobrya uambae ventral head: A, labral papillae; B, labial papillae E (left side); C, maxillary palp and sublobal plate (left side); D, proximal chaetae and basomedian and basolateral labial fields, arrow indicates M2 chaeta present or absent; E, cephalic groove and anterior part of the postlabial chaetotaxy.

FIGURE 32. Entomobrya uambae dorsal chaetotaxy (left side): A, Th II–III; B, Abd I–III; C, Abd IV–V.

FIGURE 33. Entomobrya uambae legs: A–C, chaetotaxy of subcoxa I–III respectively (outer side); D, trochanteral organ (posterior view); E, tibiotarsus II macrochaetotaxy (inner view); F, distal tibiotarsus and empodial complex I (posterior view), arrow shows the apex of ai lamella; G, distal tibiotarsus and empodial complex III (posterior view).

FIGURE 34. Entomobrya uambae abdominal appendages: A, collophore chaetotaxy (lateral view), arrows on the lateral flap indicate ciliate or smooth chaetae; B, distal manubrium (ventral view); C, manubrial plate and proximal dens (dorsal view), arrow shows the representation of the chaetotaxy; D, dens distally and mucro (outer view).

FIGURE 35. Map depicting the distribution of Entomobrya uambae in the Brazilian Amazon, the star represents its type locality and purple circles new records.