Elbamycella rosea sp. nov. A. Poli, E. Bovio, V. Prigione & G.C. Varese

Poli, Anna, Bovio, Elena, Verkley, Gerard, Prigione, Valeria & Varese, Giovanna Cristina, 2019, Elbamycellarosea gen. et sp. nov. (Juncigenaceae, Torpedosporales) isolated from the Mediterranean Sea, MycoKeys 55, pp. 15-28 : 15

Elbamycella rosea sp. nov. A. Poli, E. Bovio, V. Prigione & G.C. Varese Figures 2, 3

Type.

Italy, Tuscany, Mediterranean Sea, Elba Island (LI), Ghiaie ISL, 14-15m depth, 42°49 ’04” N, 10°19 ’20” E, on the brown alga Padina pavonica , 20 March 2010, R. Mussat-Sartor and N. Nurra, MUT 5443 holotype, living culture permanently preserved in metabolically inactively state by deep-freezing at Mycotheca Universitatis Taurinensis. A dried specimen of this culture grown on CMASS and CMASW has been deposited in the herbarium of the Department of Life Sciences and Systems Biology (TO Cryptogamia 3446).

Additional material examined.

Italy, Tuscany, Mediterranean Sea, Elba island (LI), Ghiaie ISL, 14-15m depth, 42°49 ’04” N, 10°19 ’20” E, on the seagrass Posidonia oceanica , 20 March 2010, R. Mussat-Sartor and N. Nurra, MUT 4937 = CBS 130520.

Etymology.

In reference to the colour of the colony on the culture media.

Description.

Ascomata were produced on both CMASS and CMASW at 21 °C only, after 28 days of incubation. Mycelium hyaline to pale brown consisting of smooth-walled hyphae 2.5-4 µm wide (Fig. 2 A–D, F).

Ascomata perithecial, scattered or gregarious (from 2 to 6-8), superficial, erumpent or immersed, olivaceous-brown to black at maturity, globose, subglobose, ovoid or pyriform, glabrous, up to 100-140 µm diameter; ostiolar neck, pale-coloured, single (sometimes 2, rarely 3), 55-70 µm long and 20-50 µm wide at the base; peridium 5-10 µm thick of textura prismatica in the outer layers and textura globulosa in the inner layers with cells with olivaceous-brown walls, in the neck consisting of hyaline, more elongated cells, from which numerous hyaline blunt hyphal projections 5-15 × 3-5 µm arise. Asci evanescent, hyaline, cylindrical to clavate 22-26 × 12-16 µm containing 8 spores; sterile elements not observed (Fig. 2E, G).

Ascopores cylindrical 23-28 × 4-5 µm, rounded at both ends, thin-walled, hyaline, straight or slightly curved, 3-septate, with a large basal cell 10-15 µm long and 3 shorter, upper cells, slightly constricted around the septa, the apical cell somewhat attenuated just below the blunt tip, bearing 3(4) subpolar, straight or slightly bent, acuminate, hyaline, smooth-walled cellular appendages 10-20 µm long (about 0.5-1 µm wide). In some spores the apical cell is divided by an additional septum; each cell of the spore contains a few oil-droplets 1.5-3.0 µm diameter (Fig. 2H).

Asexual morph not observed.

Colony description.

Colonies reaching 21-23 mm diameter on CMASW and 19-29 mm diameter on CMASS in 28 days at 21 °C, plane, thin, mycelium mainly submerged. Colonies pale pink in the centre becoming brown with age, colourless at the margins. Black spots due to ascomata groups in fruiting colonies. Reverse of the same colour of the surface (Fig. 3A, B).

Colonies on PDASW and PDASS reaching 10-14 mm diameter in 28 days at 21 °C, convolute, developing in height with irregular margins, salmon. Reverse of the same colour of the surface (Fig. 3C, D).

At 10 °C colony growth on all media very poor, attaining 5-8 mm diameter in 28 days. Colonies plane to slightly convolute with regular margins, pale pink to cyclamen. Reverse of the same colour of the surface (Fig. 3 E–H).