Drawida anchingiana seogwipo, Blakemore, 2013

Blakemore, Robert J., 2013, Jeju-do earthworms (Oligochaeta: Megadrilacea) - Quelpart Island revisited, Journal of Species Research 2 (1), pp. 15-54 : 19

Drawida anchingiana seogwipo subsp. nov.

[Fig. 2]

Material examined. IV0000261226 Holotype (H) figur- ed and dissected with small tissue samples taken for DNA analysis ( WO69 , w44); IV0000261228 Paratype (P) providing DNA sample ( WO71 ). Collected 9 th Sept. 2012 by RJB during short walk from Botanic Gardens to IUCN meeting at Seogwipo (33̊15′10′′N 126̊33′40′′E); preservation in 80% EtOH .

Note. Three complete specimens and two fragments, with the same collection data but none dissected, are possibly the same taxon (see “ Drawida sp.1 ” below).

Etymology. After the type-locality, noun in apposition.

Description. Length 55-58 mm (H missing tip of tail, P complete). Colour bluish in posterior of body, with faintly dark mid-dorsal line. Prolobous, ventrally cleft. No dorsal pores. Clitellum not developed. Nephropores clearly in setal d lines or above in anterior. Spermathecal pores in 7/ 8 in cd lines. Male pores on penes protruding slightly from wide slits centred on c lines in 10/11. Female pores in b lines in 11/12. Genital markings paired in 8 offset medially, and lhs analogues in 9, 10 and 11 in H; P1 has them on 10 & 11 only (circular ‘GM glands’ correspond internally - on the off-chance they are parasitic artefacts those from 8rhs and 10lhs from H sampled for DNA - w44, results in Appendix).

Internally, septa 5/6-8/9 thick; 9/10 and 10/11 are conjoined and displaced to mid-10 supporting yellow testis sac with coiled vas deferens to prostate that attaches to male organ. Dorsal blood vessel single and hearts in 6-9. Spermathecae atria present anteriorly from 7/8 with long coiled duct to ampulla attached to 7/8 posteriorly in 8. Ovisacs (empty) present from septum 12/13 that along with 11/12 forms an ovarian chamber ventrally. Nephridial bladders elongate. Gizzards muscular in 13-15.

Remarks. Differences in the current type of D. a. seogwipo from the nominal taxon are gizzards in 13-15 and lack of an atrial gland which may be its distinguishing characters, here complemented with the DNA barcode data including that of the paratype (WO71) that was not so extensively studied but differs only slightly. Morphological similarity of D. anchingiana seogwipo to D. a. halla sub-sp nov. described above, extends to atrium in 7 and lack of accessory glands there, but is contraindicat- ed by gizzards in 13-15 rather than 12-14. Slight DNA barcode indications support its separation (w24 vs. WO 69 in Appendix). However, should D. a. anchingiana proper be confirmed from China (or Jeju!) then morphological and genetic comparison may allow clarification of the full species relationship.

Both new sub-species may indicate a variable species complex, possibly with parts similar to what Kobayashi found. Mutual inclusion or separation depends on relocating fully mature material on Jeju that correspond to Kobayashi’s description, plus further studies of types of the whole species-complex in China .