Diaporthe pseudoanacardii L. Schweizer, C. Lamb. & Y. Marin, 2023

Lambert, Christopher, Schweizer, Lena, Matio Kemkuignou, Blondelle, Anoumedem, Elodie Gisele M., Kouam, Simeon F. & Marin-Felix, Yasmina, 2023, Four new endophytic species of Diaporthe (Diaporthaceae, Diaporthales) isolated from Cameroon, MycoKeys 99, pp. 319-362 : 319

Diaporthe pseudoanacardii L. Schweizer, C. Lamb. & Y. Marin sp. nov.

Fig. 6

Etymology.

Named after its close phylogenetic relation to Diaporthe anacardii .

Description.

Conidiomata pycnidial in culture on PNA, globose or irregular, dark brown to black, solitary or in groups, embedded, erumpent, 190-700(-820) μm diam, white to yellow or cream conidial drops and cirrus exuded from ostioles; conidiomatal wall pale olivaceous to olivaceous brown, composed of 1-2 layers, textura angularis. Conidiophores cylindrical to subcylindrical, base subhyaline to pale yellow or pale olivaceous, apex hyaline to subhyaline, straight, densely aggregated, smooth-walled, 1-2(-3) septate, rarely aseptate, (7.5-)10-45 × 1-3.5(-4) μm. Conidiogenous cells phialidic, cylindrical, tapering towards apex, hyaline to subhyaline, terminal or lateral, 7-28 × 1-3.5(-4) μm. Paraphyses not observed. Alpha conidia ovoid to ellipsoidal, hyaline, apex acutely rounded, base acutate, granular to guttulate, aseptate, (5-)6-8(-9) × 1.5-3 μm. Beta and gamma conidia not observed.

Culture characters.

Colonies on PDA covering the surface of the Petri dish in 2 weeks, white to grayed yellow (162C-D) or grayed white (156A-B), sometimes with transparent margins and white, yellow green (153B-C) and grayed green (195A-B) zones, granulous to cottony or slightly feathery, flat to raised in some zones, margins filamentous to fimbriate; reverse grayed yellow (161C-D or 162D) and brown (200A) or black (202A-B) center, sometimes with transparent margins. Colonies on MEA reaching 59-85 in 2 weeks, white or grayed yellow (161B-C) with normally a white ring, sometimes with grayed green zones (197A-D) and transparent margins, cottony to slightly feathery, lobate, flat to raised in some zones, margins filamentous to fimbriate; reverse grayed green (197A) to brown (200A) with grayed yellow (161B) margins, or grayed green (197A) with grayed yellow (160D) and yellow green (152B) zones and black (202A) margin, or grayed yellow (161 A-B) and transparent margins. Colonies on OA covering the surface of the Petri dish in 2 weeks, grayed green (195A-D) with white margins and yellow (4A-B) or grayed yellow (160D) center, or grayed white (156A-C) with grayed orange (163B-C) center and yellow white (158B-C) margins, cottony to slightly feathery, raised, margins filamentous to fimbriate; reverse yellow green (147B) with gray brown (199B) margins or entire gray brown (199A-B) or grayed green (195A with 198A centre).

Specimens examined.

Cameroon, Kala Mountain , from Trema guineensis , 11 Apr. 2019, E.G.M. Anoumedem (holotype CBS H-24923; culture ex-type CBS 148909 = STMA 18283) ; Tonga, West Region , from Pittosporum manii , 19 Jun. 2019, E.G.M. Anoumedem (STMA 18247, STMA 18292) .

Notes.

This species resolved in a well-supported clade (82 bs / 1 pp) together with D. anacardii , D. macadamiae , D. nebulae and D. velutina . Diaporthe pseudoanacardii can be easily distinguished from all the other species by the absence of beta conidia. All these species are reported from Africa ( Gomes et al. 2013; Lesuthu et al. 2019; Wrona et al. 2020), except of D. velutina , which was found in Asia ( Gao et al. 2017).

Figures

Figure 6. Diaporthe pseudoanacardii A, B conidioma in PNA C conidiomata in OA D, E conidiophores and conidia F alpha conidia A, C, E, F ex-type strain CBS 148909 B, D STMA 18292. Scale bars: 200 μm (A, B); 1000 μm (C); 5 μm (D-F).