Chrysozyma sambuci Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 127

Chrysozyma sambuci Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828851. Fig. 18M, N .

Etymology: the specific epithet sambuci refers to Sambucus , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are long ellipsoidal and cylindrical, 2.4– 4.0 × 7.2– 13.5 μm and single, budding is polar ( Fig. 18M ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and dull. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.2– 2.9 × 5.9 –8.8 μm ( Fig. 18N ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose, trehalose, melezitose, inulin (variable), soluble starch (variable), Darabinose (variable), ethanol (delayed and weak), D-mannitol (variable), D-glucitol (variable) and salicin (variable) are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, melibiose, raffinose, D-xylose, L-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, glycerol, erythritol, ribitol, galactitol, Methyl-α- D-glucoside, DLlactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine, ethylamine hydrochloride (weak) and cadaverine dihydrochloride (weak) are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 23–24 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ch. sambuci and its closely related species Ch. milinensis and Ch. griseoflava can be distinguished from one another by the assimilation of galactose, raffinose, D-xylose, glycerol, DL-lactate, citrate, sodium nitrite and L-lysine ( Table S1.34 ).

Typus: China, Bomi county, Tibet, obtained from a leaf of Sambucus williamsii, Sep. 2004 , F.-Y. Bai (holotype CGMCC 2.2618 T preserved in a metabolically inactive state, ex-type CBS 15450 = XZ13C5).

Figures

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.