Ceratostomella sordida (Réblová) Réblová 2024

Réblová, Martina, Nekvindová, Jana, Kolařík, Miroslav, Jurjević, Željko, Kolář, Michal & Hubka, Vít, 2024, Re-evaluation of Ceratostomella and Xylomelasma with introduction of two new species (Sordariomycetes), MycoKeys 110, pp. 319-360 : 319-360

Ceratostomella sordida (Réblová) Réblová , comb. nov.

Figs 8 , 10

Xylomelasma sordida Réblová, Mycologia 98: 88. 2006. Basionym.

Description.

See Réblová (2006).

Characteristics in culture

(after 2 / 4 wk at 23 ° C). On CMD colonies 38–40 mm / 72–73 mm diam, circular, flat, margin diffuse to slightly fimbriate, cobwebby, olivaceous-brown, reverse of the same colour. On MLA colonies 35–36 mm / 76–80 mm diam, circular, flat, sub-entire with a tendency towards a fimbriate edge, lanose, zonate, whitish grey centrally with an olivaceous brown intermediate zone, dark olivaceous grey towards the periphery, reverse dark olivaceous. On OA colonies 34–35 mm / 77–79 mm diam, flat, margin diffuse, floccose to cobwebby, olivaceous grey to olivaceous brown, aerial hyphae with numerous colourless droplets, reverse of the same colour. On PCA colonies 30–31 mm / 58–60 mm diam, circular, flat, margin rhizoidal, sparse to cobwebby, whitish brown at the centre, dark brown towards the periphery, reverse dark brown. Sporulation was absent on all media.

Temperature dependent growth at 30, 35, 37, 41 ° C was assessed as colony diam on MEA, PDA, and OA, respectively, after a period of two weeks: 30 ° C 58–60 mm / 55–58 mm / 49–50 mm, 35 ° C 60–61 mm / 58–59 mm / 46–47 mm, 37 ° C 37–39 mm / 30 mm / 14–19 mm, 41 ° C germination only / 5–7 mm / no growth.

On MLA, colonies are effuse, with submerged hyphae 1.5–3 μm in diameter; hyphae are smooth, branched, septate, subhyaline to pale brown, intertwined with dark brown, vein-like hyphae with occasional tuberose formations, 4–6.5 μm in diameter. Dark brown monilioid hyphae 5.5–9 μm in diameter, composed primarily of rectangular cells, occur rarely.

Specimen examined.

France • Pyrénés Atlantiques: Ariège, Lescure, Bois du Pas du Baup ; 500 m alt.; on rotten wood of Alnus glutinosa ; 24 Feb 2004; J. Fournier J. F. 04020 (holotype PRM 902275 !, ex-type culture CBS 116000 ) .

Habitat and geographical distribution.

Saprobe that decomposes the wood of Alnus glutinosa , Eucalyptus viminalis , Fagus sylvatica , Populus sp. and other unidentified hosts. It has been found in Argentina, Canada, Czech Republic, Denmark, France, Hungary, New Zealand and Norway ( Réblová 2006; MyCoPortal). According to GlobalFungi, C. sordida is distributed worldwide in temperate, subtropical and tropical regions of Asia, Australasia, Europe and North and South America. Identical sequences were found in 126 samples isolated mainly in cropland and forest habitats, but also in air, dust, deadwood, grassland, roots, shoots, soil (including rhizosphere soil), tundra, water, and aquatic and anthropogenic habitats in Argentina, Austria, Brazil, Canada, China, Costa Rica, France, French Guyana, Italy, Indonesia, Japan, New Zealand, Papua New Guinea, Romania, Russia, Spain, Sweden, Switzerland, South Korea, and the USA (California, Florida, Iowa, Louisiana, North Carolina, Oklahoma, Pennsylvania, and Tennessee).

Notes.

In culture, C. sordida rarely forms short monilioid hyphae (Fig. 8 ), in contrast to C. crypta , where these hyphae form frequently and are much longer (Fig. 6 ). We re-examined the holotype of C. sordida ( Réblová 2006) , focusing on the measurements of both asci and ascospores. Despite fully rehydrating the centrum of the aged material, we observed that the ascospores were slightly smaller 8–10.5 × (3.5 –) 4–5 µm compared to the previously recorded dimensions of 9–12 × 4–6 µm. Similarly, the asci showed a narrower width of 6.5–8 µm, contrasting with the previously reported range of 7–10 (– 13) µm. For accuracy, we provide both measurements in the fresh and aged material. Two species introduced in this study, C. crypta ( CBS 131683, CBS 131684) and C. melanospora ( CBS 147993), were hidden among the herbarium material labelled as C. sordida .

Figures

Figure 6. Ceratostomella crypta (CCF 5710) A colony morphology at 37 ° C after 2 weeks on MCA, MEA, OA and PDA (from left to right) B, D – F pigmented monilioid hyphae on CA. C monilioid hyphae on MEA. Scale bars: 1 cm (A); 10 μm (B – F).

Figure 8. Vegetative mycelium of Ceratostomella spp. on MLA A, B C. melanospora CBS 147993 C-G C. sordida CBS 116000. Scale bars: 10 μm (A-G).

Figure 10. Ceratostomella sordida (A-I from holotype PRM 902275 J from ex-type strain CBS 116000) A, B ascomata C a longitudinal section of the ascomatal wall D paraphyses with ascogenous cells E-I asci with ascospores J colony morphology at 23 ° C after 4 weeks on CMD, MLA, OA and PCA (from left to right). Images: on natural substrate (A-I). Scale bars: 500 μm (A, B); 20 μm (C); 10 μm (D-I); 1 cm (J).