Ceramothyrium longivolcaniforme X.Y. Zeng, T.C. Wen & K.D. Hyde, Phytotaxa 267(1): 54 (2016)

Tennakoon, Danushka S., Thambugala, Kasun M., Jeewon, Rajesh, Hongsanan, Sinang, Kuo, Chang-Hsin & Hyde, Kevin D., 2019, Additions to Chaetothyriaceae (Chaetothyriales): Longihyalospora gen. nov. and Ceramothyrium longivolcaniforme, a new host record from decaying leaves of Ficus ampelas, MycoKeys 61, pp. 91-109 : 91

Ceramothyrium longivolcaniforme X.Y. Zeng, T.C. Wen & K.D. Hyde, Phytotaxa 267(1): 54 (2016) Figure 2

Description.

Epiphytic on decaying leaves of Ficus ampelas Burm.f. Covering the upper leaf surface with dark mycelium without penetrating host tissues. Mycelial pellicle elongate, subiculum-like, comprising hyphae that are mostly narrow, 3.5-4.5 μm wide (x- = 3.8 μm, n= 20), brownish, slightly constricted at the septa, dense, radiating outward, anastomosing at the tips with cells of the hyphal network. Sexual morph: Ascomata 130-180 μm high, 200-250 μm diam. (x- = 155 × 220 µm, n = 10) in diameter, superficial, solitary, pale brown, globose to subglobose, coriaceous, somewhat flattened when dry, covered by a mycelial pellicle, with a circumferential space filled with sparse mycelium around the mature ascomata. Peridium 18-25 μm wide (x- = 23.5 μm, n= 20), light brown, with compressed, hyaline, inner cells of textura angularis and light brown outer cells of textura angularis. Asci (62 –)70– 90 × 30-60 μm (x- = 81 × 44 µm, n = 20), 8-spored, bitunicate, broadly obovoid, short pedicellate, apically rounded, with well-developed ocular chamber. Ascospores 30 –45(– 47) × 8-16 μm (x- = 36 × 12 µm, n = 30), crowded or overlapping, irregularly triseriate, hyaline, oblong to ellipsoid, muriform, with 7 transversal septa and 6 longitudinal septa, slightly constricted at the septa, smooth-walled, surrounded by a mucilaginous sheath. Asexual morph: Not observed.

Culture characteristics.

Colonies on PDA reaching 3 mm diameter after 2 weeks at 25-30 °C, slow growing, spreading, with folded, velvety, wavy margin, consist of dark mycelium, colony color from above: olivaceous green; colony color from below: dark brown to black, not producing pigments in PDA.

Material examined.

Taiwan, Chiayi, Fanlu Township area, Dahu forest, decaying leaves of Ficus ampelas Burm.f ( Moraceae ), 20 June 2018, D.S. Tennakoon, H10 (MFLU19-0823), living culture (MFLUCC19-0252).

Notes.

In this study, a sample of Ceramothyrium longivolcaniforme was collected from dead leaves of Ficus ampelas ( Moraceae ) in Taiwan. The new collection shares a close phylogenetic relationship with Ceramothyrium longivolcaniforme (MFLU16-1306) ( Figure 1 ). The morphology of our collection (MFLUCC19-0252) fits with the type material of Ceramothyrium longivolcaniforme (MFLU16-1306) in having elongate mycelial pellicle, broadly obovoid, short pedicellate asci and hyaline, oblong to ellipsoid, muriform ascospores with a mucilaginous sheath ( Zeng et al. 2016). However, the ascospores are slightly larger (30-45 × 8-16 μm) than MFLU16-1306 (28-37 × 7-13 μm) (Table 2 ). Ceramothyrium longivolcaniforme has been previously reported from Thailand on unidentified sp. (not F. ampelas ) and thus, we provide the new host record of Ceramothyrium longivolcaniforme on Ficus ampelas ( Moraceae ). Remarkably, this is the first Ceramothyrium species collected from Taiwan.

Figures

Figure 1. RAxML tree based on a combined dataset of ITS, LSU and SSU partial sequences of 45 taxa. Bootstrap support values for maximum likelihood (ML and, maximum parsimony (MP) values higher than 60 % and Bayesian posterior probabilities (BYPP) greater than 0.90 are given above each branch respectively. The new isolates are in red. Ex-type strains are in bold. The tree is rooted by Leptoxyphium fumago (CBS 123.26) and L. madagascariense (CBS 124766).

Figure 2. Ceramothyrium longivolcaniforme (MFLU 19 - 0823, new host record). a, b Appearance of colony (black spots) on host leaf c mycelial pellicle d vertical section through ascoma e section of peridium f-i asci j-m ascospores n ascospore stained in Indian ink showing mucilaginous sheath o germinating ascospore p, q colony from above and below. Scale bars: 50 µm (d), 10 µm (e), 20 µm (f-i), 10 µm (j-o).