Bulleribasidium phyllostachydis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 90-91

Bulleribasidium phyllostachydis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828765. Fig. 8J .

Etymology: the specific epithet phyllostachydis refers to Phyllostachys , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are subglobosal, ovoid and ellipsoidal, 2.6 –4.8 × 3.7– 11.3 μm and single, budding is polar ( Fig. 8J ), a sediment is formed. After 1 mo at 17 °C, a pellicle and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellow, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, cellobiose, trehalose, melibiose, raffinose, melezitose, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine (weak), N-Acetyl-D-glucosamine (weak), galactitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside (weak), salicin (weak) and Dgluconate are assimilated as sole carbon sources. L-sorbose, maltose, lactose, inulin, soluble starch, methanol, ethanol, glycerol, erythritol, ribitol, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and L-lysine (delayed and weak) are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Bu. phyllostachydis differs from its closely related species Bu. setariae in its inability to assimilate maltose, inulin, DL-lactate, succinate and citrate ( Table S1.10 ).

Typus: China, Motuo county, Tibet, obtained from a leaf of Phyllostachys sp. , Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5812 T preserved in a metabolically inactive state, ex-type CBS 15575 = XZ139E1).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.