Branchinecta uruguayensis, Rogers, D. Christopher & Lorenz, Helen Racz, 2015

Rogers, D. Christopher & Lorenz, Helen Racz, 2015, A new species of Branchinecta (Crustacea: Anostraca) from Uruguay, Zootaxa 4033 (2), pp. 280-286 : 280-283

Branchinecta uruguayensis n. sp.

Figure 1 .

Types. Holotype, male, data: URUGUAY: Rocha Department: Barra de Valizas: cultured from eggs collected from a temporary pool, November 2007, D. Dasis, deposited: USNM no. 1283171. Allotype, female; same data as holotype. Deposited: USNM no. 1283172. Paratypes: same data as holotype, 2 females, 2 males; Deposited: USNM no. 1283173.

Type locality. The type locality was a seasonally astatic aquatic pool in saline, sandy soils near the sea, in a livestock pasture in the city limits of the resort town Barra de Valizas (also known as Fondo de Valizas). The greatest depth was 10cm, and it held water in the summer months. This site was destroyed for urban development. Soil from the site was collected (by HRL) in the hope that should this species prove to be extinct in the wild, captive cultures can be maintained.

Etymology. The specific epithet “ uruguayensis ” denotes that this species is named in honor of Republica Oriental del Uruguay, and refers to the fact that this is the first species of Anostracan reported from this nation. The gender is masculine.

Diagnosis. Male ( Figs 1 A–I). Second antennal proximal antennomere with an anteriobasal protuberance and the medial surface with oblique rows of spines, the largest spines medially. Distal antennomere with an anteriobasal lobiform projection, variously developed.

Female ( Figs 1 J–K). Second antennae medial surface with proximal transverse grooves. Thoracic segments III through XIII each bearing a lateral spiniform projection. Abdominal segments I and II laterally with a crenulate posterior margin.

Description. Average length of preserved material: 10.1 mm from apex of head to apex of telson.

Male. Anterolateral corner of the head not projecting over base of eyestalk ( Fig. 1 A). Eye plus peduncle four fifths as long as first antenna. Eye large, nearly as wide as base of second antenna. Labrum smooth. First antenna approximately 30% the length of second antennae proximal antennomere. Second antennae capable of extending back to thoracopod IV or V.

Second antennal proximal antennomere with an anteriobasal protuberance bearing fine, scattered setae ( Fig. 1 B). Pulvillus and apophysis lacking. Anterior surface with a longitudinal ridge and a subdistal, anteriolateral protuberance. Medial surface bearing oblique, broken rows of spines, with mesad and distad most spines largest. Spine rows continuing onto medial side of anterior longitudinal ridge. Anteriolateral protuberance with transverse rows of scaliform spinulae. Second antennal distal antennomere approximately two thirds the length of proximal antennomere, flattened laterally, arcing medially in the proximal third, medial third straight, and apex curved medially ( Figs 1 A, C). Distal antennomere bearing an anteriobasal, lobiform projection, generally directed anteriorly, but may be directed dorsally and laterally ( Figs 1 A, B, E–G). Lobiform projection may be small, rounded, as long as broad, twice as long as broad, or four times as long as broad. Antennomere concave laterally, convex medially. Apex rounded, lacking a rasp.

Maxillae lobiform with 4 to 6 apical setae.

Abdominal segments smooth. Thoracopods serially homologous. Thoracopod V praeepipodite broadly oval with a serrate margin ( Fig. 1 L). Epipodite elongated and oval. Epipodite narrow, apically subacute. Exopodite broadly oval, margined in plumose setae. Endopodite subtriangular, apex subacute, curving dorsally. Endopodite margined on distal third with plumose setae, remaining margins with stout, pectinate spines, decreasing in length towards endites. Endite I and II with long plumose setae. Endite III with two rows of spines: anterior row bearing three short spines, two long pectinate spines, then two short spines; posterior row composed of long plumose setae. Endite IV with two rows of spines: anterior row bearing three short spines, three long pectinate spines, then two short spines; posterior row composed of long plumose setae. Endite V with two rows of spines: anterior row bearing three short spines, six long pectinate spines, then two short spines; posterior row composed of long plumose setae.

Everted gonopods short, extending to second abdominal segment ( Figs 1 H, I). Gonopods each with a single medial basal rounded projection. Everted gonopod basally with a medial rounded projection. Everted gonopod apex with one chitinized, transverse crest. Crest dorsolateral to the apical gonopore, bearing five or six conical spines, the distal most spine largest. Testes extend anteriorly to thoracic segment VIII and posteriorly to abdominal segment III.

Telson as typical for genus. Cercopods margined with plumose setae, and of proportions typical of the genus.

Female. Head smooth, anteriolateral corners projecting over eyestalks ( Fig. 1 J). First antenna three quarters the length of second antenna. Second antenna subcylindrical, proximally with a series of transverse striae, and distally a medially directed bulge, bearing a few small spines. Apex directed laterally and subaciculate. Second antennae with a few scattered spinules medially and laterally. Head without dorsal cornified protuberances. Thorax smooth dorsally, lateral margins of thoracic segments III through XIII bearing a triangular spine, distal most spines being largest ( Fig. 1 K).

Maxillae and thoracopods as in male.

Brood pouch fusiform, extending to abdominal segment IV or V ( Fig. 1 K). Ovaries extend anteriorly to thoracic segment VIII and posteriorly to abdominal segment III.

Abdominal segments I and II with lateral margins crenulate, with ventral most crenulation largest. All crenulations rounded.

Telson normal. Cercopods plumose.

Egg. Subspherical, with obtuse angles, diameter approximately 240 µm. Surface with few, large, angular depressions, up to 50 µm across ( Fig. 1 M).

Comparisons. Branchinecta uruguayensis n. sp. is most similar to B. achalensis César, 1985 . Males are separated by the presence of an anteriobasal protuberance bearing fine, scattered setae on the second antennal proximal antennomere in B. uruguayensis n. sp., and the presence of rounded mounds ( Cohen 2012) on the anterior surface of the head in B. achalensis . Additionally, the subdistal, anteriolateral protuberance (“sensory bulge” in Cohen 2012) is transverse, directed distally, and located in the distal third of the second antenna proximal antennomere in B. uruguayensis n. sp., whereas in B. achalensis it is conical, directed anteriorly, and placed just distad of the antennomere midway point.

Male B. uruguayensis n. sp. are separated from all other reported Branchinecta species by the oblique rows of spines on the second antennae, as well as the anteriobasal lobiform projection (“horn” or “big linguiform branching” in Cohen 2012) on the distal antennomere. Male B. uruguayensis n. sp. superficially resembles Branchinecta ferrolimneta Rogers & Ferreira, 2007 , which has a similar second antennal anterior ridge, but lacks the oblique spine rows and the lobiform projection. Branchinecta uruguayensis n. sp. share the distal antennomere lobiform projection (“linguiform branching” of Cohen 2012) with B. papillata Rogers, De los Rios, & Zúñiga, 2008 and B. achalensis . Both species have medial spines, but in B. papillata the spines are very broad basally.

Female B. uruguayensis n. sp. have the first two abdominal segments with the distolateral margins crenulate, while in B. achalensis the first four segments have each posteriolateral margin produced as a single angular projection. The ovaries in B. uruguayensis n. sp. extend posteriorly to abdominal segment III, whereas in B. achalensis they extend to abdominal segment V or VI.

Branchinecta uruguayensis n. sp. females are readily separated from all other Branchinecta by the lateral spines on the thorax and the lateral crenulations on the abdomen.

Branchinecta uruguayensis n. sp. appears to form a species group with B. achalensis , B. vuriloche Cohen, 1985 , B. papillata , and B. ferrolimneta . This group is characterized by the second antenna proximal antennomere bearing an anterior longitudinal ridge and the medial surface bearing transverse or oblique rows of spines that extend on to the medial surface of the ridge, as well as the form of the distal antennomere, which curves medially and tapers to an apex that is not bent medially against the antennomere’s primary axis. This species group should be referred to as the Branchinecta vuriloche species group.

Distribution and habitat. Branchinecta uruguayensis n. sp. is so far known only from the type locality. As the type locality has been destroyed, it is possible that this species is extinct in the wild. However, the region around the type locality has not been surveyed; it may be that B. uruguayensis n. sp. is more widely distributed. Thus, we take a conservative approach and treat this species under the IUCN Red List criteria as Critically Endangered (CR), with the area of occupancy less than 10 km 2, known only to exist at a single site (B2), and observed decline in extent of occurrence, occupancy and quality of habitat (ab(i, ii, iii)). At this time, as far as we know, this species only occurs in culture.

Figures

FIGURE 1. Branchinecta uruguayensis n. sp. A) Male, left side of head anterior view. B) Same, lateral view. C) Second antenna distal antennomere, lateral view. D) Same, distoanterior view. E – G) Variation in development of second antenna, distal antennomere anteriobasal lobiform projection, all lateral view. H) Gonopod, everted, ventral view. I) Detail of gonopod crest. J) Female, left side of head anterior view. K) Female, right lateral view. L) Fifth thoracopod, anterior view. M) Egg.