Bletilla formosana

Wang, Rongbin, Qin, Yadong, Zhou, Juanjuan, Wang, Juan, Shu, Huiyun, Zhou, Shoubiao & Peng, Xin, 2022, Comprehensive evaluation of Bletilla striata and its substitutes by combining phenotypic characteristic, chemical composition, and anti-melanogenic activity, Phytochemistry (113059) 195, pp. 1-11 : 4

2.5. Anti-melanogenic effects and toxicity of B. striata , B. ochracea , and

B. formosana in zebrafish embryos

In addition to estimating the potential of B. striata , B. ochracea , and B. formosana for further medical application as depigmenting agents, we further evaluated its anti-pigmentation ability through in vivo zebrafish model, and compared their depigmenting efficacy with winona. As seen from Table 3, there was no obvious histological abnormality when the concentrations of B. striata , B. ochracea , and B. formosana were below 62.5 μg/mL, respectively. Therefore, the MTC of anti-melanogenic experiment was determined as 62.5 μg/mL.

After the incubation of zebrafish embryos from 6 hpf to 54 hpf, B. striata , B. ochracea , and B. formosana exhibited an obvious pigmentation inhibitory activity at concentrations of 31.25 μg/mL compared to control group, with the pigmentation level markedly decreases about 19%, 14%, and 20%, respectively ( Fig. 7 , and Table 4). There was no significant difference between B. striata and B. formosana treatment groups. However, the average anti-melanogenic effect in B. ochracea was the lowest among them(see Fig. 8 ). The results were consistent with the clustering results of characteristic components of B. striata , B. ochracea , and B. formosana . The above results revealed quite similar depigmenting potency and toxicity profile of B. striata , B. ochracea , and B. formosana at the doses of 15.625–250 μg/mL.

Figures

Fig. 7. Anti-melanogenic effects comparison of Control(A),Winona(B), B. striata (C), B. formosana (D), and B. ochracea (E). Values given are the means ±SD (n =30). *P <0.05, ***P <0.001 versus control group.

Fig. 8. Total optical density of melanin(A) and anti-melanogenic effect(B) comparison of Control, Winona, B. striata (SR), B. formosana (FS), and B. ochracea (OS). Values given are the means ± SD (n = 30). *P <0.05, ***P <0.001 versus control group.