Arthrostoma supriatnai, Dewi & Purwaningsih & Hasegawa, 2023

Dewi, Kartika, Purwaningsih, Endang & Hasegawa, Hideo, 2023, Arthrostoma supriatnai sp. nov. (Nematoda: Ancylostomatidae) parasitic in Mydaus javanensis (Carnivora: Mephitidae) from Mount Ciremai, Java, Indonesia, Zootaxa 5353 (1), pp. 89-95 : 90

Arthrostoma supriatnai sp. nov.

( Ancylostomatidae : Ancylostomatinae : Ancylostomatini: Arthrocephalinii)

( Figs. 1a–l ; 2a–d )

General: Small and stout worm, cuticle transversely striated with striae 2.5–2.9 (2.7) apart. Anterior end curved dorsally. Mouth opening directed antero-dorsally, surrounded by four papillae and two amphids ( Fig. 1b , 2a ). Buccal capsule pyriform in lateral view, composed of ten articulating sclerotized plates ( Figs. 1a, c, d ), consisting of one large basal plate (B), two lateral plates (D), two laterodorsal (E), two lateroventral (C), one large triangular ventral plate (A), and two additional lateral plates (X). Mouth lacking teeth or cutting plates, but with one pair of ventral lancets ( Figs. 1a, c ). Dorsal esophageal gland well developed with duct passing in dorsal gutter ( Fig. 1c ). Esophagus club shaped, slightly swollen posteriorly, terminating in a lobed valve at junction with intestine; intestinal diverticulum present ( Fig 1a ). Cervical papillae finger-shaped ( Fig. 2b ).

Male: Total length 2.92 (2.72–3.11) mm; maximum width at level of posterior end of esophagus 304 (282–320), buccal capsule 101 (99–102) deep, 64 (63–66) wide. Nerve ring, excretory pore, and cervical papillae 311 (306– 318), 318 (305–330), and 418 (396–435) from anterior end, respectively. Esophagus length 515 (480–590), width 118 (111–124). Copulatory bursa symmetrical with large lateral lobes and small dorsal lobe, prebursal papillae small ( Fig. 1h ). Ventral rays stout, parallel, close together, cleft at middle of length. Lateral rays divergent from each other, externolateral shortest, mediolateral almost same length with posterolateral. Externodorsal rays derived from base of dorsal ray. Dorsal ray long, bifurcated near distal end, each branch bifid into outer and inner offshoots, and each inner offshoot divided again apically ( Fig.1j ). Simple genital cone present with one pair of tiny dorsal raylets and one pair of ventral cuticular appendages. Spicules 372 (350–390) long, equal and similar, slender tubular, transversely striated alae, with sharply pointed tip ( Fig. 1k ). Gubernaculum arrow-shaped, 104 (102–105) long ( Fig. 1l ).

Female: Total length 3.51 (3.28–3.77) mm; maximum width 323 (310–336) at level of vulva. Buccal capsule 120 (119–122) deep. Nerve ring, excretory pore, and cervical papillae 349 (315–405), 367 (339–430), and 476 (463–497) from anterior end, respectively. Esophagus length 675 (600–690), width 137 (123–147). Vulva at 1.44 (1.35–1.61) mm from posterior end, with large and protruding prevulval flap and postvulval spherical swelling ( Figs. 1f , 2c ). Vagina short, uteri opposed. Tail 360 (324–390) long, tapering to elongate, sharp terminal spike ( Figs. 1e , 2d ). Eggs not embryonated in uteri, oval, thin–shelled, 62 (60–65) x 39 (30–50) ( Fig. 1g ).

Figures

FIGURES 1a– l. Arthrostoma supriatnai sp. nov. collected from Mydaus javanensis (Carnivora: Mephitidae) caught on Mount Ciremai, Java, Indonesia. a. Anterior portion, lateral view; b. Cephalic end, apical view; c. Lateral views of buccal capsule; d. ventral views of buccal capsule; e. Female posterior end, lateral view; f. Vulva, lateral view; g. Egg; h. Bursa, ventral view; i. Genital cone; j. Dorsal ray, ventral view; k. Spicule, lateral view; l. Gubernaculum, ventral view. Scale bars: a, c, h, k. 100 µm; b, i. 10 µm; d, e, f. 50 µm; g, j, l. 25 µm. Abbreviations: de. duct of dorsal esophageal gland; dg. dorsal gutter; la. lancet.

FIGURES 2a–d. Scanning electron microscopy images of Arthrostoma supriatnai sp. nov. collected from Mydaus javanensis (Carnivora: Mephitidae) caught on Mount Ciremai, Java, Indonesia. a. Cephalic end, apical view; b. Cervical papilla; c. Vulva, ventral view; d. Female posterior end, lateral view. Scale bars: a, b. 10 µm; c. 5 µm; d. 100 µm. Abbreviations: am. amphid; cp. cephalic papillae.