Arenibacillus arenosus, Kim & Kanjanasuntree & Kim & Lee & Sukhoom & Kantachote & Kim, 2019

Kim, Jong-Hwa, Kanjanasuntree, Rungravee, Kim, Dae-Hoon, Lee, Jung-Sook, Sukhoom, Ampaitip, Kantachote, Duangporn & Kim, Wonyong, 2019, Arenibacillus arenosus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from sea sand, International Journal of Systematic and Evolutionary Microbiology 69 (1), pp. 153-158 : 157

DESCRIPTION OF ARENIBACILLUS ARENOSUS SP. NOV.

Arenibacillus arenosus sp. nov. (a.re.no′ sus. L. masc. adj. arenosus sandy, dwelling in marine sediment, sand).

Cells are rod-shaped, 0.1–0.9×2.6–3.4 µm, Gram-stain-negative, non-motile and non-spore-forming. Colonies on MA are cream-coloured, circular, smooth, convex and 0.1– 0.2 mm in diameter after 3 days of incubation at 30 Ǫ C. Growth occurs at 20–37 Ǫ C, but not at 4 Ǫ C or above 40 Ǫ C; optimum growth is at 30 Ǫ C. Growth occurs at pH 6.5–10.0, but not at pH 6.0 or pH 10.5; optimum growth is at pH 8.5. Growth occurs in the presence of 0–4 % (w/v) NaCl with optimum growth at 1 % (w/v) NaCl, but not at above 5 % (w/v) NaCl. Negative results in tests for slime production, intracytoplasmic membrane system and growth under photoautotrophic and photoheterotrophic conditions. Bchl a is not present. Indole production is negative and Voges– Proskauer reaction is positive. Reduces nitrate to nitrite. Aesculin is hydrolysed but starch, casein and gelatin are not. Citrate is not utilized. The following substrates are utilized as sole carbon sources: D- mannose, D- mannitol, L- arabinose, D- ribose and D- xylose. The following substrates are not utilized as sole carbon sources: D- arabinose, D- adonitol, D-galactose, D- glucose, D- fructose, D- fucose, D- mannose, L-sorbose, L- rhamnose, D- sorbitol, melibiose, cellobiose, maltose, lactose, D- arabitol, potassium 2-ketogluconate and potassium 5-ketogluconate. In the API ZYM system, alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, naphthol-AS-BI-phosphohydrolase, a- glucosidase and b- glucosidase show positive results, but cystine arylamidase, trypsin, a- chymotrypsin and acid phosphatase are negative. The respiratory quinone is Q-10. The polar lipid pattern consists of phosphatidylglycerol, phosphatidylcholine and an unidentified aminolipid. The predominant cellular fatty acid (>10 % of the total) is C 18: 1 Ɯ 7 c.

The type strain is CAU 1304 T (= KCTC 42827 T = NBRC 113022 T), isolated from sea sand collected in Eurwangri in the Republic of Korea. The genomic DNA G+C content of the type strain is 55.9 mol% .