Triplectides puri, Henriques-Oliveira & Nessimian & Takiya & Santos, 2025

Henriques-Oliveira, Ana Lucia, Nessimian, Jorge Luiz, Takiya, Daniela Maeda & Santos, Allan Paulo Moreira, 2025, Hidden diversity of the long-horned caddisfly genus Triplectides Kolenati, 1859 (Trichoptera: Leptoceridae) in Brazil revealed by DNA and morphology: new species descriptions and larval associations, Arthropod Systematics & Phylogeny 83, pp. 757-796 : 757-796

publication ID	https://doi.org/10.3897/asp.83.e158227
publication LSID	lsid:zoobank.org:pub:9E2BC1CD-E4CD-4287-B495-E79A2C8ED096
DOI	https://doi.org/10.5281/zenodo.17961789
persistent identifier	https://treatment.plazi.org/id/FFDC4A51-284A-508E-A198-6D46B020437A
treatment provided by	Arthropod Systematics & Phylogeny by Pensoft
scientific name	Triplectides puri
status	sp. nov.

Treatment

3.2. 8. Triplectides puri sp. nov.

Figures 22 View Figure 22 , 23 View Figure 23 , 24 View Figure 24

Henriques-Oliveira et al. 2020: 46 [as Triplectides misionensis Holzenthal, 1988 View in CoL ].

Type material.

Holotype: BRAZIL • ♂; Espírito Santo, Dores do Rio Preto, Pedra Menina, Parque Nacional do Caparaó, Rio São Domingos, Cachoeira da Farofa ; 20°28′19.20″S 41°49′41.90″W; alt. 1,964 m; 25 Jan. 2014; white sheet; A. L. H. Oliveira, J. L. Nessimian leg.; [DNA voucher ENT 3220]; DZRJ TRICHOPTERA 9229 GoogleMaps . — Paratypes: BRAZIL • 1 ♀; same data as for holotype; DZRJ TRICHOPTERA 9323 GoogleMaps • 1 larva; same data as for holotype; 27 Mar. 2012; A. LH. Oliveira leg.; [DNA voucher ENT 3231]; DZRJ TRICHOPTERA 9230 GoogleMaps • 4 larvae; same data as for preceding; DZRJ TRICHOPTERA 9235 GoogleMaps • 2 larvae; same data as for preceding; MNRJ GoogleMaps • 1 ♂: Minas Gerais, Alto Caparaó, PARNA do Caparaó, Cachoeira Bonita ; 20°24′4.9″S 41°50′13.3″W; alt. 1,709 m; 05 Oct. 2010; L. L Dumas, J. L. Nessimian leg.; DZRJ TRICHOPTERA 9231 GoogleMaps • 1 ♂: Minas Gerais, Alto Caparaó, PARNA do Caparaó, 2 nd order tributary of Rio José Pedro ; 20°24′35.00″S 41°50′56.70″W; alt. 1,792 m; 04 Apr. 2016; JL Nessimian, A. L. H. Oliveira, A. Antunes, A. A. Alves, J. Queiroz leg.; light trap; DZRJ TRICHOPTERA 9232 GoogleMaps • 1 ♂: Minas Gerais, Alto Caparaó, PARNA do Caparaó, Vale Encantado, Rio José Pedro ; 20°24′38.1″S 41°50′03.6W; alt. 1,912 m; 05 Oct 2010; B. Clarkson, I. C. Gonçalves leg.; MNRJ GoogleMaps • 1 ♂: Minas Gerais, Alto Caparaó, PARNA Caparaó, Vale Verde, Rio Caparaó ; 20°25′11.6″S 41°50′44.8″W; alt. 1,306 m; 05 Oct. 2010; L. L. Dumas, J. L. Nessimian leg.; DZRJ TRICHOPTERA 9234 GoogleMaps .

Description.

Adult male. General color golden brown (in alcohol). Antennae, palps, and legs, golden brown. Wings brown to golden brown. Forewings with forks I and V present (Fig. 22 A View Figure 22 ); discoidal cell slightly enlarged apically, cross vein s almost straight, cross vein r-m short and positioned anteriorly to m-cu (Fig. 22 D View Figure 22 ) or both cross veins almost aligned (Fig. 22 C View Figure 22 ). Hind wings broad, with forks I, III, and V present (Fig. 22 B View Figure 22 ); fork I petiolate (Fig. 22 E View Figure 22 ). Length of forewing 11–12 mm, length of hind wing 8–10 mm (n = 4). Tibial spur formula 2, 2, 4. — Genitalia: Segment IX annular, in lateral view, narrow, enlarged dorsally with setose area (Fig. 23 A View Figure 23 ); in dorsal view, produced mesally and laterally, bearing setose area laterally; dorsal process absent (Fig. 23 B View Figure 23 ). Preanal appendages digitate, long, extending beyond half the length of tergum X, setose, in lateral view (Fig. 23 A View Figure 23 ). Tergum X, in lateral view, elevated at base with median area less sclerotized than apical area (Fig. 23 A View Figure 23 ); in dorsal view, wide at base, narrower at apex, apicomesal incision wide reaching less than half the length of the tergum X and forming an oval opening, apex rounded (Fig. 23 B View Figure 23 ). Inferior appendages, long, extending beyond tergum X, bearing very long setae (Fig. 23 A, C View Figure 23 ); 1 st article, in lateral view, wide at base, slightly constricted at middle-length, with apical portion narrow and apically rounded (Fig. 23 A View Figure 23 ); apicodorsal lobe digitate, long, extending beyond 2 nd article, with very long setae (Fig. 23 A, C View Figure 23 ); basoventral lobes digitate, long, extending beyond the insertion of 2 nd article, rounded and bearing long setae (Fig. 23 A, C View Figure 23 ); in ventral view, mesal lobe shorter than basoventral lobe, basal portion subrectangular, apicomesal corner produced in a digitate process, narrow and acute apically, lateral margin almost straight apically (Fig. 23 C View Figure 23 ); 2 nd article slightly widened at base, slender, curved inwards, narrowing to an acute apex (Fig. 23 C View Figure 23 ). Phallic apparatus simple, tubular, with phallotremal sclerite small, spine-like, positioned apically (Fig. 23 D, E View Figure 23 ). — Adult female. General color pale brown (in alcohol). Antennae, palps, and legs, pale brown. Length of forewing 11.0 mm, length of hind wing 9.0 mm (n = 1). Tibial spur formula 2, 2, 4. — Genitalia: Sternum VIII, in ventral view, with a subrectangular, sclerotized plate; anterior margin with a concave, and posterior margin straight (Fig. 24 A View Figure 24 ). Segment IX sclerotized dorsally, posterior margin subtruncate, and slightly rounded. Preanal appendages, in dorsal view, small, sub-oval, setose; in lateral view, subtriangular, roof-shape (Fig. 24 B View Figure 24 ), without sensilla-bearing processes (Fig. 24 A, B View Figure 24 ). Lamella oval, flap-like, and internally concave; in ventral view directed mesad (Fig. 24 B View Figure 24 ). Gonopod plate subtrapezoidal, slightly sclerotized, with apicomesal process short and slightly rugose. Spermathecal sclerite broad, bell-sharped in ventral view, and elongate in lateral view. (Fig. 24 A View Figure 24 ). — Larva. Length up to 12 mm (n = 3) (Fig. 25 A View Figure 25 ). — Head: Coloration (in alcohol) dark brown, almost homogeneous, with pale oval area around stemmata (Fig. 25 B View Figure 25 ), subrectangular, slightly flattened dorsally (Fig. 25 B View Figure 25 ). Muscle scars reddish brown. Labrum yellowish brown, sub-oval (Fig. 25 B View Figure 25 ). Mandible asymmetrical, dark, typical for Triplectides . Submentum rectangular. Ventral apotome short, subtriangular, anterior portion widened and posterior portion narrowed and truncate (resembling a champagne flute glass) (Fig. 25 B View Figure 25 ). — Thorax: Pronotum brown, with muscle scars pale; anterior margin crenulate, lateral margin slightly produced (Fig. 25 C, D View Figure 25 ). Mesonotum pale brown, almost covered by a pair of large sclerites: sa 1 each with a single seta; sa 2 each with 3 setae: (1 mesal and 1 lateral mesal long setae, and 1 posterior very short); sa 3 each with 13–17 setae. Metanotum weakly sclerotized, covered by 3 pair of sclerites: sa 1 pair subquadrate, bearing each a long seta, sa 2 pair subquadrate, with a pair of long setae, sa 3 sclerites elongate, oval, bearing each with 7–10 setae. Prosternum trapezoidal. Mesosternum with a pair of subrectangular sclerites, curved laterally (Fig. 25 E View Figure 25 ). Metasternum with a setal area bearing 7 setae (Fig. 25 E View Figure 25 ). Foretrochantin with anterodorsal margin almost straight, with corner pointed and upturned, anteroventral margin straight and corner rounded (Fig. 25 C View Figure 25 ). Legs yellowish brown, setose (Fig. 25 G View Figure 25 ). — Abdomen: Gills simple, present on segments II – VI; segments II – IV with dorsal, lateral, and ventral filaments; segment V – VI with dorsal and lateral filaments (Fig. 25 H View Figure 25 ). Segments III – VIII with lateral fringes. Segment VIII with a pair of posteromesal short setae. Segment IX dorsal sclerite with 6 long setae on posterior margin and 2 pairs of very short, lateral setae behind those, and one anterolateral short seta at each side of the sclerite (Fig. 25 F View Figure 25 ); anal claws single, large, and pointed with a very small dorsal accessory hook (Fig. 25 F View Figure 25 ). — Larval case: Length up to 20 mm. All larvae of T. puri sp. nov. were found occupying a discarded case of Marilia sp. ( Odontoceridae ) (Fig. 25 I View Figure 25 ). — Pupa. Unknown.

Etymology.

The specific epithet ‘ puri ’ comes from the Coroado indigenous language, meaning audacious. The Puris are a Brazilian indigenous group belonging to the Macro-Jê linguistic branch, originally inhabiting the ES, RJ, MG, and SP States in southeast Brazil.

Distribution.

Brazil ( Espírito Santo and Minas Gerais states).

Habitat.

This species was found inhabiting wide streams, with crystalline waters, and stony bottoms, with many rapids and marginal vegetation composed of Atlantic Forest or highland vegetation (campos de altitude).

Remarks.

The male of Triplectides puri sp. nov. can be confused with the one of T. misionensis Holzenthal, 1988 by the mesal lobe of the inferior appendages with a narrow apical portion (Fig. 22 A View Figure 22 ). Based on the original description of T. misionensis provided by Holzenthal (1988) and based on photographs of the holotype (USNMENT 01028388), deposited in the National Museum of Natural History ( NMNH), Washington, DC, USA, we found consistent differences on this structure between these two species. In T. misionensis the mesal lobe is broad basally with a small, sclerotized basomesal point and a very narrow and divergent apical portion, but in T. puri sp. nov., the mesal lobe has the apical portion much more acute and short and the basal portion larger and subrectangular (Fig. 22 A View Figure 22 ). The larva of the new species can be distinguished from its congeners based on the following characteristics: head dark brown, almost homogeneous, subrectangular, slightly flattened dorsally (Fig. 24 B View Figure 24 ); labrum yellowish brown, subtrapezoidal, submentum rectangular and ventral apotome short, subtriangular, like a Champagne flute glass, wide at anterior portion and posterior portion truncate (Fig. 24 B View Figure 24 ). DNA sequences were obtained only for two specimens, an adult male and a larva, but they share the same haplotype for both gene fragments sequenced, thus allowing us to associate this larva.