Diaporthe multiguttulata F. Huang, K.D. Hyde & Hong Y. Li, in Huang et al., Fungal Biology 119(5): 343. 2015.

Yang, Qin, Jiang, Ning & Tian, Cheng-Ming, 2021, New species and records of Diaporthe from Jiangxi Province, China, MycoKeys 77, pp. 41-64 : 41

Diaporthe multiguttulata F. Huang, K.D. Hyde & Hong Y. Li, in Huang et al., Fungal Biology 119(5): 343. 2015. Figure 6

Description.

Conidiomata pycnidial, 692-750(-800) μm diam., solitary and with single necks erumpent through host bark. Tissue around neck is cylindrical. Locule circular, undivided, 450-565(-600) μm diam. Conidiophores reduced to conidiogenous cells. Conidiogenous cells unbranched, straight or slightly curved, apical or base sometimes swelling, (8.5-)9-10.5(-11) × 1.5-2 μm. Alpha conidia hyaline, aseptate, ellipsoidal, biguttulate or with one large guttulate, rounded at one end, slightly apex at the other end, occasionally submedian constriction, (7.5-)8-9(-10.5) × 4-5(-5.5) μm. Beta conidia not observed.

Culture characters.

Colony originally flat with white felty aerial mycelium, becoming pale green mycelium with age, margin area irregularly, with visible solitary conidiomata at maturity.

Specimens examined.

China. Jiangxi Province: Ganzhou City, on branches of Citrus maxima , 25°51'28"N, 114°55'19"E, 11 May 2018, Q. Yang, Y. Liu & Y.M. Liang (BJFC-S1614; living culture: CFCC 53095, CFCC 53096 and CFCC 53097).

Notes.

Diaporthe multiguttulata was originally described as an endophyte from a healthy branch of Citrus grandis in Fujian Province, China ( Huang et al. 2015). In the present study, three isolates (CFCC 53095, CFCC 53096 and CFCC 53097) from symptomatic branches of Citrus maxima were congruent with D. multigutullata , based on DNA sequence data and confirmed from the morphological analysis (Fig. 1 ). The clade, therefore, was verified as D. multigutullata which could exist both as an endophyte and a pathogen.

Figures

Figure 1. Phylogram of Diaporthe from a Maximum Likelihood analysis based on combined ITS, cal, his 3, tef 1 and tub 2. Values above the branches indicate Maximum Likelihood bootstrap (left, ML BP ≥ 50 %) and Bayesian probabilities (right, BI PP ≥ 0.90). The tree is rooted with Diaporthella corylina. Strains in current study are in blue font and the ex-type cultures are in bold font.

Figure 6. Diaporthe multiguttulata on Citrus maxima (BJFC-S 1614) a, b habit of conidiomata on twig c conidiomata on PDA d transverse section through conidiomata e longitudinal section through conidiomata f conidiogenous cells attached with alpha conidia g alpha conidia h the colony on PDA. Scale bars: 200 μm (b, d, e); 10 μm (f, g).