Korotnevella sp. 3

Udalov, Ilya A. & Völcker, Eckhard, 2022, Is scale’s structure still a good character to delimitate species of Amoebozoa? case of the genus Korotnevella (Amoebozoa, Dactylopodida), Organisms Diversity & Evolution (New York, N. Y.) 22 (1), pp. 17-33 : 24

Korotnevella sp. 3

Locomotive cells usually have an elongated triangular or rectangular shape ( Fig. 2Q, S ). Cells form subpseudopodia during locomotion ( Fig. 2Q, S ). Length of the locomotive form is 24–46 μm (average 36.6 μm), breadth is 7–22 μm (average 12.1 μm), and L/B ratio is 1.4–5.8 (average 3.3). The single nucleus of ellipsoid shape ( Fig. 2T, U ) is 3.7–5.6 μm in length (average 4.7 μm) and 2.8–3.5 μm in breadth (average 3.1 μm). The nucleolus is ball-shaped, usually with a central transparent lacuna ( Fig. 2T, U ), but in some cells, it is divided into two ball-shaped pieces ( Fig. 2T ). The diameter of the nucleolus is 1.5–2.3 μm (average 1.9 μm). In some cells, one or two contractile vacuoles are observed ( Fig. 2Q, S ).

In our cultures, amoebae form cysts with a thick cyst wall ( Fig. 2V ). The diameter of the cytoplasmic body of the encysted cell is 10 ‒ 15 μm (average 11.9 μm), while the diameter of the cyst including its wall is 12 ‒ 18 μm (average 14.5 μm).

Cell surfaces are covered with two types of scales: large basket-shaped and small dish-shaped (Fig. 3K). The length of the basket-shaped scales is 270–421 nm (average 337 nm); breadth 161–226 nm (average 186 nm); and height 119–206 nm (average 149 nm). The length of the dish-shaped scales is 35–135 nm (average 86 nm); breadth 36–96 nm (average 59 nm); and height 15–26 nm (average 20 nm). Large scales include basal plate, perforated flange, and latticework basket in the shape of a closed hammock, featuring short filaments that are connected with a wide rim (Fig. 3K, M) so as to form two short spikes at their ends.

Figures

Fig. 2 Light micrographs of Korotnevella sp. 1 (A–F), Korotnevella sp. 2 (G–K), K. hortobotanici sp. nov. (L–P), and Korotnevella sp. 3 (Q–V). Locomotive forms in a Petri dish, phase contrast (A–D, G, H, L–N, Q–S). Nucleus, DIC (E, I–K, O, P, T, U). Cyst, DIC (F,