Allodia karkloofensis Magnussen, 2018

Magnussen, Trude, Kjaerandsen, Jostein, Johnsen, Arild & Søli, Geir E. E., 2018, Six new species of Afrotropical Allodia (Diptera: Mycetophilidae): DNA barcodes indicate recent diversification with a single origin, Zootaxa 4407 (3), pp. 301-320 : 313-316

Allodia karkloofensis Magnussen sp. nov.

( Fig. 9 )

Diagnostic characters. A. karkloofensis can best be separated from the other species described here based on the following combination of characters: Length of rm is equal to the stem of posterior fork ; the dorsal lobe of gonostylus is similar to A. jaschhofi , which both have a large membranous area on the internal apex of the lobe; the shape of the median lobe is distinct, having a very rounded posteroventral corner, with one seta at the distal part of the ventral edge ( Fig. 10 ); the basal part of the gonostylus ( Fig. 9C ) is similar to that of A. jaschhofi ( Fig. 7C ), but without a small pointed structure.

Type material. Holotype ♂. South Africa: KwaZulu-Natal national park, Howik district, Karkloof Range, Geekies Farm (29.1600°S, 030.2100°E), malaise trap. 29 Feb. – 0 9 Mar. 2000. TSZD-JKJ- 101653. Leg. W. Brakemeyer. (TMU).

Measurements. Male: Body length 4.35 mm; wing length 3.04 mm.

Coloration. Head and clypeus brown. Mouthparts and palpomeres whitish yellow. Antennae brown, with scape, pedicel and first half of first flagellomere whitish yellow. Scutum brown. Lateral sclerites brown. Wings clear without markings. Halteres whitish. Legs whitish. Abdomen brown, with slightly paler area close to lateral edge of tergites. Terminalia whitish yellow.

Head. Two ocelli, located close to the eye margin. Head covered with fine trichia, except for row of about 8 short setae near eye margin, above ocellus. Antennae about twice as long as thorax. Scape and pedicel with several setae on distal third. Flagellomeres cylindrical, densely clothed with fine trichia. First flagellomere twice as long as scape.

Thorax. Antepronotum with 5 strong setae. Scutum covered with uniform small, pale setae, with strong lateral prealar and postalar setae. Discal bristles absent. Scutellum with 2 strong bristles. Laterotergite with 4 long setae and three shorter. Other lateral sclerites bare.

Legs. Fore-, mid and hind tibiae with short setae arranged in rows. Mid tibia with 9 anterodorsal and 30 posterodorsal bristles, on distal 3/4 of segment. Hind tibia with 8 anterodorsal and 8 posterodorsal bristles.

Wings. Sc short, ending in R, length of rm equal to length of stem of posterior fork, base of anterior fork slightly before base of posterior fork. R1 and R5 with tapering setae.

Terminalia male ( Fig. 9 ). Tergite 9 medially divided, each part rounded, covered with minute trichia, with two strong bristles and 8 small setae. Cerci clothed with fine trichia, with long setae apically; pseudocerci with several long setae medially and apically. Dorsal lobe of gonostylus oblong, apex prominently rounded, with large, round membranous area internally; outer surface with numerous strong setae. Median lobe with indistinct posteroventral corner, with 7 strong and 4 shorter setae on posterior margin; one small setae on surface of lobe; two long setae on ventral edge, one at basis, and one just before posteroventral corner. Ventral lobe slightly club-shaped, with about nine long setae at apex. Basal part of gonostylus with one large round structure, covered with many stout setae. Hypandrial lobe blunt, weakly sclerotized at apex ( Fig. 9A ).

Etymology. Named after the type locality.

Remarks. Measurements were made on specimen in ethanol.

CO1 sequence data. Full length, bidirectional sequences of the CO1 barcode region were successfully obtained for four of the six new species, A. nyeriensis , A. jaschhofi , A. keurbosensis and A. drakensbergensis . We were unable to obtain CO1 sequences from the two remaining new species; A. karkloofensis and A. mazumbaiensis . Interspecific p-distances among the Afrotropical species ranged between 5.6% and 11% ( Table 2). The genetic distance between the Afrotropical species and the six outgroup taxa ranged between 10.2% and 14.1%. All the Afrotropical species grouped together with respect to the outgroup taxa.

Figures

FIGURE 7. THe MALe TeRMINALIA OF A. jaschhofi. A, LeFT: DORSAL VIeW, RIGHT: VeNTRAL VIeW; B, GONOSTYLUS FROM INNeR SIDe; C, BASAL PART OF GONOSTYLUS.SCALe bAR = 1µM.

FIGURE9. THe MALe TeRMINALIA OF A.karkloofensis. A: LeFT:DORSALVIeW,RIGHT:VeNTRAL VIeW,B: GONOSTYLUS FROM INNeR SIDe, C: BASAL PART OF GONOSTYLUS.SCALe bAR = 1µM.

FIGURE10. VARIATION beTWeeN AFROTROPICAL Allodia, GONOSTYLUS FROM INNeR SIDe.A, A.nyeriensis; B, A.mazumbaiensis; C, A. drakensbergensis; D, A.keurbosensis; E, A.jaschhofi; F, A. karkloofensis. SCALe bAR = 1µM.