Hyphoderma tenue (Pat.) Donk

Cho, Minseo, Kwon, Sun Lul, Jang, Seokyoon, Yoo, Yeonjae, Lee, Sang Hyun, Kwon, Dae Young, Kim, Changmu, Lim, Young Woon & Kim, Jae-Jin, 2024, Notes of 15 unrecorded macrofungi in Korea, Journal of Species Research 13 (1), pp. 67-88 : 78

Hyphoderma tenue (Pat.) Donk ,

Fungus 27: 15 (1957) [MB#298781] ( Figs. 1I and 2I ) Korean name: ğêḵ"ậNjŀġfflŷ

Basidiome resupinate, widely effused, thin. Hymenial surface crustaceous, odontoid, cracked when old, yellowish white to cream-colored, margin white to yellowish white. Hyphal system monomitic, generative hyphae simple septate, sometimes branched, without clamp connections, thin-walled, hyaline, 3.0-5.0 μm. Basidia clavate to subclavate, 4-spored, smooth, thin-walled, hyaline, 15.5-32.0 × 5.2-9.1 μm. Cystidia obovoid, smooth, thin-walled, hyaline, 15.8-41.1 × 4.1-12.4 μm. Basidiospores ellipsoid, smooth, thin-walled, hyaline, 6.5-9.4 × 4.3-5.9 μm [Q =1.51-1.59].

Specimen examined. Korea, Gangwon-do , Sokcho-si, Seoraksan National Park, 38°10 ʹ 18.5 ʺ N, 128°29 ʹ 32.1 ʺ E, 198 m, mixed hardwood forest, occurring on the conifer branch, 19 Sep 2018, S. Jang and S. L. Kwon, KUC2018 0919-27 (NIBRFG0000514543) .

Notes - Hyphoderma tenue is characterized by crustaceous and odontoid hymenial surface, and obovoid cystidia. According to the phylogenetic analysis, H. tenue formed a monophyletic clade with high bootstrap value, and closely related with H. crystallinum and H. variolosum ( Fig. 8 ). Hyphoderma variolosum differs from H. tenue by narrower and cylindrical cystidia (40-50× 4-6 μm) ( Boidin and Gilles, 1991). In addition, H. crystallinum has two forms of cystidia; tubular and encrusted, and longer basidiospores (10.5-)11-14.5(-15) × 4-5.5(-6) μm ( Guan and Zhao, 2021), which differs from H. tenue . The molecular work in this study was conducted using LSU region, so additional analysis using the ITS region is required for more accurate identification.

Figures

Fig. 1. Macro-morphological characteristics of fifteen unrecorded macrofungi in Korea. A. Agaricus thiersii; B. Baorangia alexandri; C. Boletellus putuoensis; D. Entoloma bulakhae; E. Entoloma pygmaeopapillatum; F. Entoloma subtenuicystidiatum; G. Gerronema kuruvense; H. Hyphoderma nudicephalum; I. Hyphoderma tenue; J. Macrolepiota subcitrophylla; K. Mycena jingyinga; L. Mycena yuezhuoi; M. Ophiocordyceps vespulae; N. Scytinostroma acystidiatum; O. Steccherinum straminellum (scale bar=10 mm for A-D, G-J, M-O; 5 mm for E, F, L; and 2.5 mm for K).

Fig. 2. Micro-morphological characteristics of fifteen unrecorded macrofungi in Korea. A. Agaricus thiersii; B. Baorangia alexandri; C. Boletellus putuoensis; D. Entoloma bulakhae; E. Entoloma pygmaeopapillatum; F. Entoloma subtenuicystidiatum; G. Gerronema kuruvense; H. Hyphoderma nudicephalum; I. Hyphoderma tenue; J. Macrolepiota subcitrophylla; K. Mycena jingyinga; L. Mycena yuezhuoi; M. Ophiocordyceps vespulae; N. Scytinostroma acystidiatum; O. Steccherinum straminellum(scale bars=10μm).

Fig. 8. The phylogenetic tree of Hyphoderma nudicephalum (SFC20150108-26, SFC20150113-05, and SFC20180704-83) and H. tenue (KUC20180919-27). The tree was constructed based on ITS and LSU gene datasets of the genus Hyphoderma. Physisporinus tibeticus was used as an outgroup. The newly generated sequence is shown in blue and bold. Bootstrap support values more than 70 are shown.