Clunio balticus, Heimbach, 1978

Michalova, Paraskeva, Lencioni, Valeria, Nenov, Momchil & Nikolov, Svetoslav, 2021, Can DNA barcoding be used to identify closely related Clunio Haliday, 1855 species (Diptera: Chironomidae, Orthocladiinae)?, Zootaxa 4927 (1), pp. 1-8 : 2

Clunio balticus and Clunio ponticus sampling

Two male adults of both C. balticus and C. ponticus (preserved in 96% ethanol) were analysed. C. balticus was collected from the Atlantic Ocean seashore about 20 km south of Bergen in western Norway, in the Kviturdvikpollen (60 o 16´N, 5 o 15´E), reared by Dr. F. Heimbach and sent to Dr. P. Michailova on 09.05.1978. C. ponticus was collected while it was floating on the water surface of the Black Sea coast near Varna beach, Bulgaria, St. Konstantin and Helena Resorts (4313’45’N and 28 0’30’E) on 26.VI.2019. Head, thorax, legs and hypopygium of the examined adults (where diagnostic characters to identify the species are present) dissected and mounted on a microscope slide with Canada Balsam, after dehydration with acetic acid and clarification with butanol (Lencioni et al. 2017). Genomic DNA extracted from the abdomen. The mounted samples identified to species level according to Moubayed-Breil & Michailova (2020) and Moubayed-Breil et al. (2020). Morphological voucher specimens and the DNA vouchers of each specimen are deposited in Valeria Lencioni’s collection at the Science Museum of Trento ( Italy).