Anenterotrema liliputianum ( Travassos, 1928 ) Caballero, 1964

Lunaschi, Lía I. & Drago, Fabiana B., 2011, A revision of Anenterotrema Stunkard, 1938 (Digenea: Anenterotrematidae) and a key to its species, Zootaxa 2775, pp. 50-64 : 57

Anenterotrema liliputianum ( Travassos, 1928) Caballero, 1964

( Figure 3 ; Table 3 )

Syns. Paralecithodendrium liliputianum Travassos, 1928 ; Edcaballerotrema liliputianum (Travassos) , Freitas, 1961; Anenterotrema eduardocaballeroi ( Freitas 1960) of Freitas & Dobbin (1962) in part

Material studied. Paralectotypes CHIOC 23.658 a–d. Ex Molossidae sp. (type host), small intestine, Angra dos Reis (type locality), Rio de Janeiro, Brazil.

Hosts. Molossidae : Molossidae sp. [according Freitas (1961)]; Molossus molossus molossus (as M. major crassicaudatus ); M. molossus (Pallas) (as M. obscurus Geoff. ); M. rufus rufus ; Molossops temminckii (Burmeister) . Phyllostomidae : Phyllostomus elongatus .

Distribution. Brazil (Rio de Janeiro State: Angra dos Reis, Rio de Janeiro, Manghinhos; Pernambuco State: Recife, Escada; Argentina (Misiones Province: Reserva Valle del Arroyo Cuñá Pirú).

References. Travassos (1928); Freitas (1961); Freitas & Dobbin (1962); Ubelaker et al. (1977); Lunaschi & Notarnicola (2010).

Redescription. [Based on 4 whole-mounts] Body very small, pyriform, with maximum width in middle region; 275–362 (306) x 184–251(205). Forebody ventrally concave, 1.2–2.2 (1.5) times longer than hindbody; forebody 105–145 (121) long; hindbody 53–102 (84) long. Ratio of body length to forebody length 1:1.2–2.2 (1:1.5). Tegument unarmed. Anterior end without transverse fold of body wall at level of oral sucker. Oral sucker spherical, large, subterminal, 80–107 (90) x 93 –113(103). Ventral sucker in middle third of body, spherical, similar in size to oral sucker, 89–107 (99) x 107–121 (113). Sucker width ratio 1:0.8–1 (1:0.9). Pharynx, oesophagus and intestinal caeca absent. Gonads surrounding ventral sucker, only clearly visible in specimen CHIOC 23.658 b. Testes, small, symmetrical or sub-symmetrical, entire, lateral or posterolateral to ventral sucker; right testis 58–60 x 53 –73 (59 x 63); left testis 58–66 x 48 –56 (62 x 52). Ratio of body length to testis length 1:4.7–6.2 (1:5.3). Ratio of ventral sucker length to testis length 1:1.6–1.8 (1:1.7). Cirrus sac 1.4–2.4 times smaller than ventral sucker, entirely, or almost entirely, anterior to ventral sucker, containing long coiled internal seminal vesicle, pars prostatic, prostatic cells and cirrus, 39–79 x 54 –58 (53 x 57). Ratio of ventral sucker length to cirrus sac length 1:1.4–2.4 (1:2). Genital pore median, in forebody, halfway between suckers. Ovary submedian, posterolateral to ventral sucker, 34– 37 x 31–64 (36 x 48). Mehlis’ gland median, lateral to ovary; seminal receptacle not seen; Laurer’s canal short. Vitellarium follicular, in hindbody, masked by loops of uterus, extends across body. Uterus long, occupying all available space in hindbody and extending laterally to ventral sucker, contains numerous operculate eggs, large in relation to body size, with brown coloration, with fully-developed miracidium, 27– 32 x 17–22 (29 x 20). Ratio of body length to egg length 1:9–13 (1:10). Metraterm short, weakly developed, thin-walled, opens in shallow genital atrium, opposite to cirrus sac. Excretory pore and excretory vesicle not seen.

Remarks. Freitas (1961) transfered Paralecithodendrium liliputianum Travassos, 1928 to Edcaballerotrema Freitas, 1961, as E. liliputianum ( Travassos, 1928) Freitas, 1961 . Travassos (1928) described this species parasitizing a molossid bat from Brazil (originally reported from Pteropteryx canina Wied ), and erroneously described parenchymal cells in the forebody as vitelline follicles. Freitas (1961) studied the specimens collected by Travassos (CHIOC lectotype 23.657 and paralectoypes CHIOC 23.658 a–d) and others from M. molossus , extending the original description. Freitas & Dobbin (1962) described other specimens from M. molossus molossus and P. e l o n g a t u s, reiterating the faulty location of the vitelline follicles. Caballero (1964) transferred this species to Anenterotrema without any discussion. Yamaguti (1971) examined the type specimens and noted the confusion between the parenchymal cells of the forebody and the vitelline follicles, which are concentrated posterior to the left testis. Portes Santos & Gibson (1998) examined the lectotype CHIOC 23.657 (cited as holotype CHIOC 33.657) and concluded that it is not possible to see the vitellarium due to the great number of eggs in the uterus. Finally, Lunaschi & Notarnicola (2010) re-described this species from M. molossus and P. elongatus in Brazil and from M. temmincki in Argentina, confirming Yamaguti’s observation. Moreover, they consider as A. liliputianum the specimens CHIOC 28004, 28005 a–c and 28006 a–c described by Freitas & Dobbin as A. eduardocaballeroi from M. rufus . Of the four paralectotypes studied herein, only one exhibited the vitelline follicles (CHIOC 23.658b); in this specimen the follicles appear to extend across the body; this is probably due to the degree of contraction of the body.

Figures

FIGURE 3. Anenterotrema liliputianum. A and B paralectotypes CHIOC 23.658 d. C and D paralectotypes CHIOC 23.658 b. A – C, general view. D, enlarged view of the terminal genitalia. Scales: A – C: 100 μm; D: 50 μm.