Fungal

Bakhshi, Mounes, Arzanlou, Mahdi, Babai-Ahari, Asadollah, Groenewald, Johannes Z. & Crous, Pedro W., 2014, Multi-gene analysis of Pseudocercospora spp. from Iran, Phytotaxa 184 (5), pp. 245-264 : 246

Fungal isolation

Plant samples with cercosporoid leaf spot symptoms were collected from four provinces of Iran including Guilan, Mazandaran, Ardabil and Zanjan, taken to the laboratory, and examined under a Nikon SMZ 1500 stereo-microscope to observe sporulation. Fungal strains were isolated in pure culture by direct transfer of spores from a single leaf spot onto plates containing 2% malt extract agar ( MEA; Fluka, Hamburg, Germany) with a sterile fine pointed needle as explained in Bakhshi et al. (2011). Representative samples of diseased specimens were dried in a plant press and deposited in the Fungal Herbarium of the Iranian Research Institute of Plant Protection ( IRAN). Representative isolates of the fungi were deposited in the Culture Collection of Tabriz University ( CCTU) and the Centraalbureau voor Schimmelcultures ( CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands). Isolates included in the morphological and phylogenetic analyses are listed in Table 1.