Oxynoemacheilus banarescui ( Delmastro, 1982 )

Freyhof, Jörg, Kaya, Cüneyt & Geiger, Matthias F., 2022, A practical approach to revise the Oxynoemacheilus bergianus species group (Teleostei: Nemacheilidae), Zootaxa 5128 (2), pp. 151-194 : 167-168

Oxynoemacheilus banarescui ( Delmastro, 1982)

( Fig. 3 )

Orthrias brandti banarescui Delmastro, 1982: 53 , figs. 1–3 (type locality: Turkey: Devrekani creek near Devrekani, Kocaçay basin)

Material examined. FFR 15511, 4, 43–77 mm SL; Turkey: Kastamonu prov.: stream Devrekanı 8 km northeast of Devrekanı , 41.6275N 33.9224E .— FSJF 3119 , 30 , 31–67 mm SL; Turkey: Zonguldak prov.: Yenice River 15 km west of Yenice , 41.2325N 32.2041E .— FSJF 3122 , 6 , 64–78 mm SL; IUSHM 2018–1403 , 10 , 50–62 mm SL; Turkey: Karabük prov.: stream Davulga at 15 km north of Mengen , 41.0872N 32.0230E .— FSJF 3169 , 19 , 41–70 mm SL; Turkey: Kastamonu prov.: stream Devrekani at Kasaplar, 4 km north of Devrekani , 41.6224N 33.8526E .

Material used in molecular genetic analysis. FSJF DNA-1626 ; Turkey: Kastamonu prov.: stream Devrekani at Kasaplar, 4 km north of Devrekani , 41.6224N 33.8526E (GenBank accession: OK316781 , OK316749 ) .— FSJF DNA-1631 ; Turkey: Zonguldak prov.: Yenice River 15 km west of Yenice , 41.2325N 32.2041E (GenBank accession: OK316694 , OK316657 ) .

Distribution. Oxynoemacheilus banarescui is known from the Devrekani and Yenice/Filiyos drainages in the Anatolian Black Sea basin. See Fig. 2 for the distribution of this species.

Remarks. Oxynoemacheilus banarescui is distinguished from other species of the O. bergianus group by a minimum K2P distance of 2.9% to O. bergianus in the DNA barcode region and it is supported by PTP, mPTP and ASAP. We were not able to find a reliable, consistent external character to distinguish O. banarescui from any of the other species of the O. bergianus group.

Turan et al. (2019) published two sequences (GenBank accession number: MH469261 , MH469262 ) of O. banarescui from the stream Devrekani, closely clustering with our sequences from the same drainage. But Turan et al. (2019) submitted three additional sequences ( MH018854 , MH018855 , MH018856 ) to GenBank, which they omitted from the manuscript. Sungur (2020) downloaded all five sequences labelled as “ Oxynoemacheilus banarescui, Devrekani stream: Kızılırmak Basin” from GenBank. The Devrekani does not flow to the Kızılırmak.As Cüneyt Kaya is a co-author of the study of Turan et al. (2019) and the present study, it is now possible to clarify the reasons for this omission. Indeed, the sequences MH018854 , MH018855 , and MH018856 do not originate from fishes collected in the Kızılırmak or Devrekani drainages, but in the Büyük Melen (40.8358N 31.1100E) and it is therefore no surprise that they do not cluster with O. banarescui but with O. simavicus from the Büyük Melen in our molecular dataset ( Fig. 1 ).

Figures

FIGURE 1. Maximum likelihood estimation of the phylogenetic relationships based on the mitochondrial COI barcode region (GTR model, discrete Gamma distribution for rate differences with 5 categories +G parameter = 0.1781). Nucleotide positions with less than 98% site coverage were eliminated, resulting in 581 analysed positions. Numbers of major nodes indicate bootstrap values above 80 from 1000 pseudoreplicates from the ML, MP and NJ method. Solid black bars right to the specimen labels indicate species demarcation results from PTP followed by the results of the mPTP approach as dashed bars and theASAP partitions in grey. Note that the dashed bar left extends to the right side including O. parvinae. The tree is drawn to scale (except to the outgroup) with branch lengths depicting number of substitutions per site.

FIGURE 2. Records of species of the O. bergianus group. Different colours indicate different molecular groups within species. Grey circles indicate records of O. simavicus without assignment to a molecular group.

FIGURE 3. Oxynoemacheilus banarescui, FSJF 3122, 78 mm SL; Turkey: stream Davulga; 3169, 70 mm SL; Turkey: stream Devrekani; FSJF 3122, 78 mm SL; Turkey: stream Davulga; FSJF 3119, 67 mm SL; Turkey: Yenice River.