Diplothrombium ostovani Noei and Saboori

Noei, Javad, Saboori, Alireza & Hajizadeh, Jalil, 2014, Two new species and one new record of larvae of the family Johnstonianidae (Acari: Prostigmata) from Iran with a key to species of the genus Diplothrombium, Zootaxa 3785 (2), pp. 241-257 : 248-255

Diplothrombium ostovani Noei and Saboori , sp. nov.

Diagnosis. SD 62–69; DS 52–92; Fe I 57 –65; Ge I 25–30; Ti I 30–32; Ta I 64 –71; Ta II 54 –57; Ta III 57 –64; IP 749–788; 2b bifid and setulose, AL much longer than PL.

Description. LARVA (n = 13). Dorsum. Idiosoma 270–345 long and 220–285 wide. Dorsum of idiosoma with scutum of typical shape. Laterally on each side of scutum 1 pair of eyes, each pair on common ocular plate, anterior eye (diameter 11–12) slightly larger than posterior eye (diameter 9–11). Scutum bearing 2 pairs of normal setae (AL and PL), 2 pairs of sensilla (AM and PSens) and longitudinal bar between basis of AM and posterior margin of scutum. AL barbed and much longer than PL, PL short and smooth. Anterior sensilla very short and posterior sensilla relatively long, both smooth. Posterior to scutum rows of faintly barbed setae (DS): C row: c 1–3, D row: d 1– 3, E row: e 1–3, F row: f 1–3, H row: h 1–2, each seta placed on sclerite (fD= 28) ( Fig. 7 ). Vent er. Coxa I with setulose bifurcate setae 1b (37–47 long) and 1a (32–37) on pars medialis; supracoxal seta absent. Coxa II with setulose bifurcate seta 2b (25–37); Claparède’s organ oval between coxae I–II. Coxa III with smooth seta 3b (20–30); seta 3a setulose (25–30) located between coxae. Posterior to coxae III approximately 57 barbed setae inserted on small platelets. Anal pore without surrounding sclerites ( Fig. 8 ). Gnathosoma. Hypostomal setae (bs) bifurcate and smooth; oral setae (or) simple and smooth. Supracoxal setae of palps absent. Palp trochanter (17–25 long) without seta, palp femur (37–45) and genu (17–20) each with 1 barbed seta, palp tibia (30–32) with 3 setulose setae and distally bifid odontus, 11–12 long. Palp tarsus (52–57) considerably exceeding tibia, with single solenidion ω, 4 setulose and 3 barbed setae. Palpal setal formula (fPp) = 0-B-B-BBB2-7Bω. Cheliceral base 37–45 long; cheliceral blade curved and with 2 distinct subterminal teeth, 15–17 long ( Fig. 12 ). Legs. Leg segmentation formula 7-7-7 ( Fig. 9–11 ). Leg setal formula. Leg I: Ta- 1ω, 1ε, 2ζ, 28–31n; Ti- 2φ, 6n; Ge- 5–6σ, 4n; TFe- 2θ, 5n; BFe- 1n; Tr- 1n ( Fig. 9 ). Leg II: Ta- 1ω, 1ε, 1ζ, 21–23n; Ti- 2φ, 6n; Ge- 2σ, 4n; TFe- 1θ, 4n; BFe- 2n; Tr- 1n ( Fig. 10 ). Leg III: Ta- 14n; Ti- 1φ, 6n; Ge- 2σ, 4n; TFe- 1θ, 4n; BFe- 2n; Tr- 1n ( Fig. 11 ). IP = 749–788. Femur of legs I–III only partially divided into basifemur and telofemur. All tarsi with 2 unequal claws and without empodium. Tarsus I with 1 terminal and 1 subterminal eupathidium (ζ), on tarsus II only 1 terminal eupathidium, no eupathidia on tarsus III. Famulus on tarsus I prolonged, famulus on tarsus II of normal minute size. Solenidion (ω) on tarsus II club-shaped.

Measurements provided in Table 2 .

Etymology. This species is named in honor of Prof. Hadi Ostovan (Department of Entomology, Islamic Azad University, Science and Research Branch, Marvdasht, Iran) in appreciation of his work on taxonomy of mites in Iran.

Type material. Holotype larva (ARS-20131003-2a) and 12 larva paratypes (ARS-20131003-2b–m) from soil (off host) [forest close to river], IRAN: Guilan province, Asalem city, Gijave village, 37° 42.077' N, 48° 54.077' E, 405 m a.s.l., 12 June 2010, coll. J. Noei.

Type deposition. Holotype and 4 paratypes are deposited at the Acarological collection, Jalal Afshar Zoological Museum, Faculty of Agriculture, University of Tehran, Karaj, Iran, 4 paratypes – in the Acarological collection, Acarological Society of Iran, Karaj, Iran, and 4 paratypes – in the Acarological collection, Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, Iran.

Differential diagnosis. This species is closest to Diplothrombium cascadense in having palpal tibial claw (odontus) bifid, coxala II (2b) bifurcate and lateral coxala I (1b) bifurcate but differs from it in the number of solenidia on Ge I (5–6 vs. 8), number of normal setae on Ta III (14 vs. 16), ventral setae (barbed vs. smooth), 1a and 2b (setulose vs. smooth), 3a (barbed vs. smooth), PL (smooth vs. barbed), number of cheliceral teeth (two vs. one) and nasus (blunt vs. sharp). Also D. ostovani differs from the other species as follows: from D. monoense in the number of solenidia on Ge I (5–6 vs. 15), number of normal setae on Ta I (28–31 vs. 34), on Ta II (21–23 vs. 27) and on Ta III (14 vs. 20), 2b (setulose vs. smooth), coarse pores near posterior margin of scutum and an irregular reticulum medially behind the sensilla (absent vs. present), PL (smooth vs. barbed), number of cheliceral teeth (two vs. one); from D. creticum in the number of solenidia on Ge I (5–6 vs. 10–12), number of normal setae on Ta II (21–23 vs. 32) and on Ta III (14 vs. 26), ventral setae (barbed vs. smooth), 2b (bifid and setulose vs. simple and serrate), 3a (barbed vs. smooth), idiosomal sclerites (platelet shape vs. tubular shape); from D. rackae in the number of solenidia on Ge I (5–6 vs. 6–9), normal setae on Ta I (28–31 vs. 32–37), ventral setae (barbed vs. smooth), 2b (bifid vs. simple), PL (smooth vs. barbed), number of cheliceral teeth (two vs. one); from D. longipalpe in the number of solenidia on Ge I (5–6 vs. 9–11), number of normal setae on Ta III (14 vs. 17–18), setae in rows C and D (6 vs. more than 6), ventral setae (barbed vs. smooth), 2b (bifid vs. simple), 3a (barbed vs. smooth), AL (barbed vs. smooth), number of cheliceral teeth (two vs. one), nasus (blunt vs. sharp); from D. ludwinae in the number of solenidia on Ge I (5–6 vs. 7–8), number of normal setae on Ta III (14 vs. 16), AL (barbed vs. smooth), medial coxala I (on coxa I vs. separated from coxa I), 2b (bifid vs. simple); from D. zbigniewi in the dorsal setae (barbed vs. nude), ventral setae (all on platelets vs. only seven setae on platelets), 2b (bifid and setulose vs. simple and nude), 3a (barbed vs. nude), AL (barbed vs. smooth), PL (smooth vs. barbed), medial coxala I (on coxa I vs. separated from coxa I), nasus (blunt vs. sharp); from D. moldavicum in the transverse bar of scutum (one vs. three); from D. newelli in the number of solenidia on Ge I (5–6 vs. 8), PL (smooth vs. pectinate), medial coxala I (on coxa I vs. separated from coxa I) and shape of odontus on palp tibia (bifid vs. simple).

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*except for W & PSens which was 5 and 4 respectively and** for PSens & PW 5 and for bs 4.

Figures

FIGURE 7. Diplothrombium ostovani sp. nov. (larva), dorsal view of idiosoma.

FIGURE 8. Diplothrombium ostovani sp. nov. (larva), ventral view of idiosoma.

FIGURE 12. Diplothrombium ostovani sp. nov. (larva), dorsal (right) and ventral (left) view of gnathosoma.

FIGURES 9 - 11. Diplothrombium ostovani sp. nov. (larva). 9. Leg I; 10. Leg II; 11. Leg III.

Tables

TABLE 2. Measurements of Diplothrombium ostovani sp. nov. larvae (a, holotype; b – m, paratypes).

Character	a	b	c	d	e	f	g	h	i	j	k	l	m	Range
IL	309	285	329	272	329	297	285	309	347	334	322	297	297	272–347
IW	223	218	277	218	272	248	223	228	248	285	235	223	218	218–285
SD	67	64	62	64	62	67	64	69	69	64	69	64	67	62–69
W	52	52	50	54	50	50	50	52	52	50	52	52	52	50–54
AW	30	30	30	30	30	30	30	27	27	30	30	27	30	27–30
PW	45	42	42	47	40	40	40	45	42	42	42	42	45	40–47
AA	8	8	8	8	8	5	5	5	8	8	5	8	8	5–8
SB	30	30	30	30	30	30	32	30	30	30	30	30	32	30–32
ASB	37	40	37	40	37	35	37	40	42	37	40	37	40	35–40
PSB	27	27	25	27	25	30	27	30	27	27	30	27	27	25–30
MA	17	12	15	15	12	15	15	15	15	15	15	12	15	12–17
AP	25	22	22	27	22	25	25	27	25	25	25	25	25	22–27
AL	50	54	52	47	50	47	50	50	52	50	50	50	52	47–54
PL	17	20	12	22	17	15	20	15	17	17	17	17	17	12–22
AM	12	10	15	10	10	10	10	12	12	12	10	10	12	10–15
PSens	99	64	94	92	99	94	104	92	97	99	99	99	99	64–104
DS	62–84	62–87	62–92	52–82	67–92	54–82	52–84	62–84	59–87	62–87	62–87	62–87	62–87	52–92
PDS	69	62	67	54	57-67	62	74	69	69	74	67	67	62	54–74
1a	37	35	35	35	37	35	37	37	37	35	35	32	37	32–37
1b	45	40	37	40	45	45	37	45	45	40	47	45	45	37–47
2b	30	35	32	25	35	35	30	35	37	32	35	35	35	25–37
3a	27	27	25	25	30	27	25	25	25	27	27	25	25	25–30
3b	25	25	20	22	25	30	25	20	25	25	25	25	25	20–30
or	12	12	10	10	12	12	12	15	12	12	12	12	12	10–12
bs	20	17	15	20	17	20	20	20	17	22	20	20	20	15–22
Cx I	50	52	47	47	50	47	50	45	52	52	50	47	50	47–52
Tr I	27	27	25	25	27	25	25	25	27	25	25	25	25	25–27
BFe I	30	32	30	30	32	30	35	30	30	30	32	32	30	30–35
TFe I	30	27	30	30	30	30	30	28	30	28	30	30	28	27–30
Ge I	27	27	25	25	27	25	30	27	30	27	30	27	27	25–30
Ti I	30	30	30	32	30	30	32	32	30	30	30	32	30	30–32
Ta I (L)	69	71	67	64	69	69	71	69	69	69	69	71	69	64–71
Ta I (H)	35	30	30	32	32	32	32	30	32	32	32	32	32	30–32
Leg I	260	264	251	279	265	255	267	252	262	262	261	259	258	251–279
Cx II	54	54	57	57	54	54	54	52	54	59	52	54	59	52–59
Tr II	27	22	25	25	25	25	25	25	25	25	25	25	25	22–27