Conlarium baiseense L.Xie, Y.L.Chen & B.Liu

Xie, Ling, Chen, Yan-Lu, Long, Yan-Yan, Zhang, Yan, Liao, Shi-Tong, Liu, Bin, Qin, Li-Ping, Nong, Qian & Zhang, Wen-Long, 2019, Three new species of Conlarium from sugarcane rhizosphere in southern China, MycoKeys 56, pp. 1-11 : 4-5

Conlarium baiseense L.Xie, Y.L.Chen & B.Liu sp. nov. Figure 2

Etymology.

The species is named for Baise City, the type locality.

Type.

CHINA. Guangxi: Baise City, Tiandong County, Silin Town . 23°30'38"N, 107°20'1"E, 109 m alt., in sugarcane rhizosphere, 11 Sep 2015, Y.L. Chen and L.P. Qin, TD2 ( HMAS 247298 , holotype) deposited in Microbiology Research Institute, Guangxi Academy of Agricultural Science.

Description.

Colony reached 14 mm diameter on medium after 2 weeks at 28 °C, grey-white to grey, circular, flat growth, less aerial hyphae, regular edge of colony. Hyphae light yellow-green to light yellow-brown, septate. Conidiophores yellow-brown, mostly stubby, 0-2-branched, 0-8-septate, straight or flexuous, 3-12 × 2-6 μm (7 ± 2 × 4 ± 1 μm, n = 51). Conidiogenous cells determinate, doliiform, yellowbrown to brown, 3-8 × 5-12 μm (6 ± 1 × 7 ± 2 μm, n = 51). Muriform conidia yellow-brown to brown, irregularly globose or subglobose, smooth, constricted at the separation, 0-1 transversely septa, 0-4 longitudinal septa, 15-25 × 12-19 μm (18 ± 2 × 15 ± 2 μm, n = 26). Columnar conidia, yellow-brown to brown, 2-5 transversely septa, no longitudinal septa, 21-35 × 7-12 μm (28 ± 5 × 10 ± 1 μm, n = 23). Sexual morph: undetermined.

Habitat and distribution.

In sugarcane rhizosphere soil of southern China.

Other specimens examined.

CHINA. Guangxi: Baise City, Tiandong County, Silin Town. 23°30'3.68"N, 107°20'1"E, 112.5 m alt., in sugarcane rhizosphere, 11 Sep. 2015, Y.L. Chen and L.P. Qin, TD17 ( HMAS 247986).

Notes.

Conlarium baiseense is similar to the asexual morph of C. aquaticum , C. duplumascospora , C. nanningense , and C. thailandense . They all have monoblastic, holoblastic, conidiogenous cells and mostly irregular, brown, clathrate, muriform conidia ( Liu et al. 2012). However, C. baiseense can be easily distinguished from C. aquaticum , C. duplumascospora , C. nanningense , and C. thailandense by its conidial septa number (6-12-transverse septa, 4-10-longitudinal septa in C. aquaticum ; 2-4-transversely septate, 1-3-longitudinally septate in C. duplumascospora ; 0-1 transversely septa, 0-4 longitudinal septa in C. nanningense ; 4-8-transverse septa, 4-6-longitudinal septa in C. thailandense vs 0-2 transversely septa, 0-8 longitudinal septa in C. baiseense ) and conidial size (15.5-35 × 11-26.5 μm in C. duplumascosporum , 45-70 × 20-57 μm in C. aquaticum , 25-45 × 17-33 μm in C. thailandense , 11-21 × 9-21 μm in C. nanningense vs 21 × 35-7 × 12 μm in C. baiseense ) ( Liu et al 2012; Zhang et al. 2017; Phookamsak et al. 2019). Phylogenetic reconstructions based on SSU+ITS+LSU+RBP2 sequences shows that authentic C. baiseense form independent monophyletic groups, well separated from C. aquaticum , C. duplumascospora , C. nanningense , and C. thailandense , respectively. A comparison of ITS sequence shows that C. baiseense differs from C. aquaticum , C. duplumascospora , C. nanningense , and C. thailandense in 26 bp, 24 bp, 18 bp, and 24 bp, respectively. According to the guidlines in Jeewon and Hyde (2016), we introduce C. nanningense as a new species.