Toxoplasma gondii

Blaga, Radu, Aubert, Dominique, Thébault, Anne, Perret, Catherine, Geers, Régine, Thomas, Myriam, Alliot, Annie, Djokic, Vitomir, Ortis, Naïma, Halos, Lénaïg, Durand, Benoît, Mercier, Aurélien, Villena, Isabelle & Boireau, Pascal, 2019, Toxoplasma gondii in beef consumed in France: regional variation in seroprevalence and parasite isolation, Parasite (Paris, France) 26 (77), pp. 1-14 : 7-9

Toxoplasma gondii isolation and genotyping

The digestion products of 209 heart samples, obtained from both serologically positive (n = 200) and negative (n = 9) animals, were inoculated in mice. In total, 627 mice were used for the bioassay. For four bovine samples (8, 76, 122, 125 months; terminal titer of 10, 10, 25 and 10, respectively), all three mice died in the first 48 h post-inoculation (p.i)., yielding 205 bioassay results. For two samples, corresponding to serologically Note. % prevalence, 95% CI: 95% confidence interval of prevalence. * One information missing (adults/veal), one is added to total.

positive adult bovines (4.8 and 7 years old; terminal titer of 25 and 10), collected in Franche-Comté and LanguedocRoussillon region slaughterhouses, we noted seroconversion in the inoculated mice 4 weeks p.i.; a microscope examination

at 60 days p.i. confirmed the presence of tissue cysts in brains. Both strains were genotyped as type II with all 15 microsatellite markers ( TUB2 , W35, TgM-A, B18, B17, M33, MIV.1, MXI.1, M48, M102, N60, N82, AA, N61, N83) being

amplified. They can be found in the Toxoplasma Biological Resource Center, Reims, France (toxocrb.com) under accession numbers: TgA 32127 and TgA 32128. No strains were isolated from the heart samples corresponding to the nine serologically negative bovines. Detailed data about the number of collected and bioassayed samples of French origin according to the terminal titer + and age can be found in the Supplementary file.