Paramirandina guttulata H. W. Shen, K. D. Hyde & Z. L. Luo, 2024

Shen, Hong-Wei, Bao, Dan-Feng, Wu, Na, Al-Otibi, Fatimah, Luo, Zong-Long & Hyde, Kevin D., 2024, Paramirandina guttulata (Microthyriaceae, Microthyriales), a new lignicolous freshwater fungi from a plateau lake in Yunnan Province, China, Biodiversity Data Journal 12, pp. e 137989-e 137989 : e137989-

Paramirandina guttulata H. W. Shen, K. D. Hyde & Z. L. Luo sp. nov.

Materials

Type status: Holotype. Occurrence: catalogNumber: KUN-HKAS 131771 ; occurrenceRemarks: on unknown decaying plant branch submerged in a lake; recordNumber: L 2204; recordedBy: H. W. Shen; sex: anamorph; lifeStage: asexual; occurrenceID: 605EBD3A-853F-50BB-8A11-8FA4C5C39F82; Taxon: kingdom: Fungi; phylum: Ascomycota; class: Dothideomycetes; order: Microthyriales ; family: Microthyriaceae ; genus: Paramirandina ; specificEpithet: guttulata ; taxonRank: species; verbatimTaxonRank: sp.; nomenclaturalCode: ICN; Location: country: China; stateProvince: Yunnan; county: Jingdong; locality: Dujuanhu Lake ; verbatimElevation: 2500 m; verbatimLatitude: 24 ° 32 ′ 22.76 ″ N; verbatimLongitude: 101 ° 1 ′ 6.85 ″ E; Identification: identifiedBy: H. W. Shen; Z. L. Luo; Event: year: 2023; month: 2; day: 24; habitat: Freshwater; Record Level: institutionID: KUN-HKAS; institutionCode: Herbarium of Kunming Institute of Botany, Academia Sinica ( KUN-HKAS)

Description

Saprobic on submerged decaying wood in a freshwater lake. Sexual morph: Undetermined. Asexual morph: Hyphomycetous (Fig. 1 ). Colonies on natural substrates effuse, hairy, mostly in small groups, sometimes scattered, dark brown, with whitish conidial masses at the apex. Mycelium mostly immersed, composed of branched, septate, hyaline to brown, smooth-walled hyphae. Conidiophores (230 –) 264–310 (– 330) × 4.5–5 (– 6) µm (x ̄ = 287 × 5 µm, n = 35), macronematous, mononematous, erect, gregarious, unbranched, cylindrical, straight to flexuous, septate, gradually tapering from the base to the apex, with the base transitioning from dark brown to hyaline towards the apex. Conidiogenous cells (31 –) 40–60 (– 70) × 4–5 µm (x ̄ = 50 × 5 µm, n = 20), polyblastic, integrated, terminal, indeterminate, sympodial, cylindrical, pale brown to hyaline, smooth. Conidia (31 –) 33–38 (– 44) × 6–7.5 µm (x ̄ = 35 × 7 µm, n = 40), acropleurogenous, solitary or in chains, fusiform, cymbiform, fusoid-clavate, straight or slightly curved, truncate at the base, obtuse at the apex, hyaline, 4–6 - septate, smooth, guttulate, sometimes bearing a new conidium at the apex.

Culture characteristics: Conidia germinating on PDA medium and germ tubes produced from both ends of conidium within 12 h. Colonies on PDA medium reaching 1–1.5 cm diam. after one month at room temperature (around 25 ° C) in dark, circular, with dense, velvety, pale brown to brown mycelium from above, brown from below.

Material examined: CHINA, Yunnan Province, Pu’er City, Jingdong Yi Autonomous County, Dujuanhu Lake, 24 ° 32 ′ 22.76 ″ N, 101 ° 1 ′ 6.85 ″ E, on unknown decaying wood submerged in Dujuanhu Lake, H. W. Shen, 24 February 2023, L 2204 (KUN-HKAS 131771, holotype), ex-type living culture DLUCC 2204.

Etymology

“ guttulata ” refers to guttulate conidia of the species.

Notes

Paramirandina guttulata closely resemble P. aquatica and P. cymbiformis . However, they can be distinguished from each other by several key morphological characteristics. Paramirandina guttulata can be distinguished from P. aquatica by its gregarious, longer conidiophores (264–310 × 4.5–5 µm vs. 138–200 × 4.5–8 μm) and slightly larger conidia (33–38 × 6–7.5 µm vs. 23–34 × 4–7.5 μm) ( Liu et al. 2023). Paramirandina guttulata differs from P. cymbiformis in having gregarious conidiophores and larger conidia (33–38 × 6–7.5 µm vs. 24–30 × 5–6.5 µm) ( Liu et al. 2023). Comparisons of nucleotide base of ITS and LSU sequence data between P. guttulata and P. aquatica showed 7.5 % (51 / 531 bp, including 11 gaps) and 1.6 % (13 / 813 bp, without gaps) differences, respectively. The LSU sequences between P. guttulata and P. cymbiformis showed 1.6 % (12 / 768 bp, without gaps) nucleotide base differences. Following the guidelines provided by Chethana et al. (2021), P. guttulata is introduced as a new species from the plateau lakes in Yunnan Province, China, based on its unique morphological characters and phylogenetic analysis.

Figures

Figure 1. Paramirandina guttulata (HKAS 131771, holotype) a, b Colony on natural substrate; c, d Conidiophores with conidia; e, f Conidiogenous cells; g, h Conidia; i Germinated conidium; j, k Culture on PDA medium. Scale bars: c, d = 100 μm, e – i = 20 μm.