Phytomyza, Fallen, 1810

Guglya, Yuliia, 2021, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of seven new species, Zootaxa 5014 (1), pp. 1-158 : 82-84

Phytomyza pulmonaria Nowakowski

( Figs. 226–235 , 634–639 )

Material examined: Ukraine: Kharkiv Region: near Petrivske , 49°10’N, 36°58’E, 2.vii.2019, Yu. Guglya, ex C ynoglossum officinale (2♂) ; same locality, 10.vi.2020, Yu. Guglya, ex Myosotis sparsiflora (1♀) ; Kharkiv, City Centre , 50°00’N, 36°14’E, 14.vii.2016, S. Chervonyak, ex Symphytum asperum (1♂) ; same locality, 23.vi.2013, Yu. Guglya, ex Symphytum asperum (1♀) ; same locality, Yu. Guglya, ex Symphytum asperum (7♂ 6♀) ; Kharkiv, Piatykhatky , 49°10’N, 36°58’E, 18–21.vi.2017, Yu. Guglya, ex S ymphytum asperum (2♂ 2♀) .

Hosts. Boraginaceae : Symphytum L., Cynoglossum L., Pulmonaria L., Myosotis L. ( Benavent-Corai et al. 2005).

Mine. ( Figs. 226, 231 ) The larva produces a whitish linear-blotch mine. Several larvae develop in one leaf, and with time neighbouring mines unite to form a large blotch that obliterates the initial linear portions. Pupation takes place outside or within the mine on the underside of the leaf ( Figs. 227, 232 ).

Puparium. ( Figs. 228–230, 233–235 ) Yellow to orange, silky-shining, 2.1 mm long, with distinct but shallow segmentation; surface quite smooth except for wide bands of fine brown spines. One row of sparse larger brown spines encircles each segment medially. Posterior spiracles set on stout conical protuberances that are entirely separate; brown, glossy, with 11–12 fine brown sessile bulbs set in a kidney-like configuration. Ventral portion of the last abdominal segment slightly protruding posteriorly viewed from the side. Anal plate orange, distinctly protruding above the surface of the puparium viewed from the side and directed ventro-posteriorly.

Cephalopharyngeal skeleton. ( Fig. 634 ) Right mouthhook larger than the left, each with abducted portion directed ventro-anteriorly and bearing two accessory teeth. Intermediate sclerite long and straight, with long and sharp protuberance directed ventro-posteriorly; sclerite 1.8× as long as maximum height of left mouthhook. The mouthhook and intermediate sclerite are strongly sclerotized and the pharyngeal sclerite much less so. The ventral cornu bears a narrow “closed” window located centrally. Indentation index 81.

Female head. ( Figs. 635, 636 ) Black, with frons, lunule, face and gena brown, proboscis yellowish; orbit not projecting above eye in profile; 2 orb s, 1 fr s; lunule broad, narrowing posteriorly, not reaching the level of fr s; pped of medium size, rounded; gena medially 0.23× as high as maximum height of eye.

Female genitalia. ( Figs. 637–639 ) Capsule of spermatheca relatively small, 0.18× as high as height of anterior part of oviscape. Spermathecae equal in size, dark brown, flattened basally and apically, wider than high. Internal duct invagination cylindrical, as deep as height of spermatheca. Spermathecal duct weakly sclerotized. Ventral receptacle S-shaped, with well sclerotized tail that is two-bladed basally. Body of receptacle hemispherical with uniformly curved basal connecting tube, strongly sclerotized, equal in diameter with capsule of spermatheca; with opening 0.4× as wide as diameter of spherical part of body.

Distribution. Germany, the Republic Moldova, Poland ( Pape 2014). Ukraine (first record).

Figures

FIGURES 221–235. Figures 221–225: Phytomyza nowakowskiana Beiger; 221–222: mine in Symphytum cordatum leaf; 223: empty puparium viewed from the side; 224: posterior segments of puparium (posterior view); 225: posterior spiracles (posterior view). Figures 226–230: P. pulmonaria Nowakowski, reared from Myosotis sparsiflora; 226: mine; 227: puparium in mine; 228: empty puparium viewed from the side; 229: posterior segments of puparium (posterior view); 230: posterior spiracles (posterior view). Figures 231–235: P. pulmonaria Nowakowski, reared from Symphytum asperum; 231: mines; 232: puparia in mine; 233: empty puparium viewed from the side; 234: posterior segments of puparium (posterior view); 235: posterior spiracles (posterior view).

FIGURES 634–639. Phytomyza pulmonaria Nowakowski; 634: cephalopharyngeal skeleton; 635: frontal view of head; 636: head viewed from the side; 637: oviscape, ventral receptacle and spermathecae proportionally; 638: spermatheca; 639 a–b: ventral receptacle; a: lateral view; b: ventro-lateral view.