Neodactylaria simaoensis, H. Zheng & Z.F. Yu, 2020

Qiao, Min, Zheng, Hua, Lv, Ruili & Yu, Zefen, 2020, Neodactylariales, Neodactylariaceae (Dothideomycetes, Ascomycota): new order and family, with a new species from China, MycoKeys 73, pp. 69-85 : 69

Neodactylaria simaoensis, H. Zheng & Z.F. Yu sp. nov. Fig. 2

Diagnosis.

It is characterised by straight or flexuous, 2-4-septate, unbranched conidiophores, with denticulate conidiogenous cells and obclavate to long obpyriform, subulate or slightly rostrate towards the obtuse or rounded apex and 1-2 (-3)-septate conidia. Differs from N. obpyriformis by longer and slightly wider conidia and more septa.

Type.

China, Yunnan Province, Simao country, 100°59'19"N, 22°46'38"E, ca 1330 m alt., from submerged unidentified dicotyledonous leaves, 28 Oct 2013, Z.F. Yu, live culture YMF 1.03984 - holotype, dried slide YMFT 1.03984.

Description.

Mycelium partly superficial or partly immersed, composed of branched, septate, hyaline to subhyaline, creeping, 1.0-2.0 μm wide hyphae. Conidiophores macronematous, mononematous, straight or flexuous, slightly geniculate towards the apex, 2-4-septate, unbranched, hyaline or pale brown, 38-86 (-129) × 3-4 μm, arising from the creeping hyphae pale brown. Conidiogenous cells polyblastic, indeterminate, sympodial extended, integrated, terminal or intercalary, denticulate with protuberant cylindrical denticles. Conidia solitary, obclavate to long obpyriform, subulate or slightly rostrate towards the obtuse or rounded apex, lumina micro-guttulate, 1-2 (-3)-septate, constricted at the septa, pale to mid brown, 15-40 × 3.6-6.5 μm, with a subhyaline, protuberant basal hilum up to 1 μm long.

Culture characteristics.

Colonies attaining 1 cm in diameter on CMA after 7 days at 25 °C. On CMA, colonies flat, floccose at the centre, lacking aerial mycelium towards periphery, white to cream-coloured, reverse same colour, sporulation abundant. On PDA, colonies flat, white to cream-coloured, margin entire; sporulation sparse.

Habitat and distribution.

In submerged dicotyledonous leaves from south-western China.

Teleomorph.

Not known.

Etymology.

The species epithet indicates its occurrence in the county of Simao, China.

Notes.

Based on a Blast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequences of N. simaoensis (GenBank MH379209) is N. obpyriformis (GenBank NR_154267, Identities = 545 / 569(96%), Gaps = 4 / 569(0%)). Morphologically, the new species, N. simaoensis , shares several characters with N. obpyriformis (type species): both have white to cream-coloured colonies, with short-cylindrical denticles as conidiogenous cells and obpyriform to slightly rostrate conidia ( Crous et al. 2017). However, N. simaoensis differs from N. obpyriformis by having obviously longer and slightly wider conidia (15-40 × 3.6-6.5 μm vs 10-14 × 3-5 μm) and more septa.

Figures

Figure 2. Culture and anamorph of Neodactylaria simaoensis (YMF 1.03984) A culture on CMA B-D conidia E conidia and conidiophores F immature conidium and conidiogenous cells G conidiophores and conidia under low power microscope. Scale bars: 1 cm (A); 10 μm (B-F); 50 μm (G).