Henlea perpusilla Friend, 1911

Lu, Yajing, Xie, Zhicai & Zhang, Junqian, 2018, Preliminary taxonomical investigation of soil enchytraeids (Clitellata, Enchytraeidae) from south region of Tibet, China, Zootaxa 4496 (1), pp. 395-410 : 403-405

Henlea perpusilla Friend, 1911

( FigURE 4G–J )

Henlea perpusilla Friend, 1911 . Černosvitov 1937; Nielsen & Christensen 1959; Chalupský 1986; Kasprzak 1986; Rota & Healy 1994; Rota 1995; Rota et al. 1998; Wang et al. 1999; Schmelz & Collado 2010.

Henlea brucei Stephenson, 1922 .

Henlea balcanica Černosvitov, 1930 .

Examined material. XZO201604001, XZO201604002, XZO201604003, ThREE adUlTS, FiXEd, STaiNEd aNd WhOlEmOUNTEd SPEcimENS.

Description. BOdY lENgTh 5–6.4 mm (5–10 mm iN ViVO). SEgmENT NUmbER 30–38. BOdY WidTh 158–210 Μm aT V aNd 175–290 Μm aT XII (256–280 Μm aNd 340–460 Μm iN ViVO). ChaETaE FORmUla (2)3-5 – 3-5: 3-6,(7,8) – 2- 5,(7,8), STRaighT diSTallY, SlighTlY bENT PROXimallY. PREcliTEllaR EPidERmal glaNd cEllS cONSPicUOUS, abOUT 3 TRaNSVERSE ROWS PER SEgmENT. BRaiN iN II, WEaklY cONcaVE aNTERiORlY, SlighTlY iNciSEd OR TRUNcaTE POSTERiORlY, 120 Μm iN lENgTh

aNd 94 Μm iN WidTh. ThREE PaiRS OF PhaRYNgEal glaNdS iN IV–VI, all WiThOUT dORSal cONNEcTiON. OESOPhagEal aPPENdagES PRESENT iN VI, WiThOUT bRaNchES. INTESTiNal diVERTicUla abSENT. GUT WidENiNg cONSPicUOUSlY aT VII/VIII ( Fig. 4G, J ). CliTEllUm ElEVaTEd cONSPicUOUSlY, aT XII–XIII, cEllS iN RETicUlaTE PaTTERN, hYalOcYTES iSOlaTEd ( Fig. 4H ). DORSal blOOd VESSEl FROm VIII. FiVE PaiRS OF PREcliTEllaR NEPhRidia iN VI/VII–X/XI. COElOmOcYTES abUNdaNT, ROUNd OR OVal, 22–26 Μm iN SiZE ( Fig. 4G ). SPERm FUNNEl Small, cYliNdRical, cOllaR NaRROWER ThaN FUNNEl bOdY, lENgTh 1.5– 2 TimES aS lONg aS WidTh, 50–86 Μm iN lENgTh aNd 36–57 Μm iN WidTh ( Fig. 4I ). VaS dEFERENS lONg, WOUNd iN iRREgUlaR lOOPS iN XII. SEmiNal VESiclE WEll-dEVElOPEd, OccUPYiNg iN XI, SOmETimES EXTENdiNg FORWaRdS iNTO X. HEadS OF SPERmaTOZOa 25–30 Μm. SPERmaThEca iN V. NO EcTal glaNdS. EcTal dUcT 68–81 Μm lONg aNd 9–12 Μm WidE. AmPUlla WiThOUT diVERTicUla, SlighTlY WidER ThaN EcTal dUcT, 25–30 Μm lONg aNd 15 Μm WidE. ENTal dUcT 84–96 Μm iN lENgTh aNd 7–11 Μm iN WidTh, UNiTEd PROXimallY aNd cONNEcTiNg JOiNTlY WiTh OESOPhagUS dORSallY iN VI. USUallY 1 OR 2 maTURE EggS aT a TimE.

Differential diagnosis. OUR SPEcimENS cONFORm WEll TO ThE dEScRiPTiON OF Henlea perpusilla ( ROTa 1995, SchmElZ & COlladO 2010). ThiS SPEciES iS WidESPREad iNclUdiNg iN AmERica, EUROPE aNd ThE ANTaRcTic. IN ChiNa, ThE WORmS WERE FOUNd iN GUiZhOU, HUNaN, JiliN PROViNcES aNd TibET.

Figures

FIGURE 4. Micrographs of Fridericia peregrinabunda Michaelsen, 1913 (A–F) and Henlea perpusilla Friend, 1911 (G–J). Fridericia peregrinabunda: A. Coelomocytes (black arrows) in vivo. B. Oesophageal appendages with branches (black arrow) in vivo. C. Pharyngeal glands in vivo. D. Head pore (black arrow), brain (white arrow) in vivo. E. Spermathecae of fixed specimen. F. Nephridium (black arrow), coelomo-mucocytes (white arrow) of fixed specimen. Henlea perpusilla: G. Coelomomucocytes (black arrow), intestine widening in VII/VIII (white arrow) in vivo. H. Clitellar glands, dorsal view in vivo. I. Sperm funnels (black arrows) in vivo. J. Abrupt transition between oesophagus and intestine at VII/VIII of fixed specimen. Scale bars: A,H: 25 µm; B,E,F,G,I: 50 µm; C,D,J: 100 µm.