Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips, Stud. Mycol. 55: 248. (2006).

Li, Wen-Li, Liang, Rui-Ru, Dissanayake, Asha J. & Liu, Jian-Kui, 2023, Botryosphaerialean fungi associated with woody oil plants cultivated in Sichuan Province, China, MycoKeys 97, pp. 71-116 : 71

Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips, Stud. Mycol. 55: 248. (2006).

Fig. 15

Fusicoccum parvum Pennycook & Samuels, Mycotaxon 24: 455. 1985. Basionym.

Botryosphaeria parva = Botryosphaeria parva Pennycook & Samuels, Mycotaxon 24: 455. 1985.

Description.

Saprobic on decaying branches of Idesia polycarpa . Sexual morph: Ascomata 284-321 × 129-223 μm (x̄ = 302.5 × 176 μm, n = 10), pseudothecial, forming a botryose aggregation of up to 30, solitary or gregarious, stromatic, immersed, partially erumpent when mature, dark brown to black, more or less circular, multiloculate, individual locules 143.5-161 μm diam, thick-walled. Peridium 59-78 μm diam., composed of several layers of thick-walled, pale brown cells of textura angularis. Ostiole 43.5-58 μm wide, circular, central, papillate. Asci 95-99 × 20-21.5 μm (x̄ = 97 × 20.5 μm, n = 30), (6-)8-spored, bitunicate, fissitunicate, cylindrical to clavate, apex rounded with an ocular chamber, sometimes short pedicellate. Ascospores 18.5-23 × 7-10.5 μm (x̄ = 20.5 × 9 μm, n = 30), L/W ratio = 3, fusoid to ovoid, with tapered ends and appearing spindle-shape, hyaline, aseptate, externally smooth, internally finely verruculose, biseriate in ascus. Asexual morph: Not observed.

Culture characteristics.

Ascospores germinate on PDA within 12 h. Colonies growing on PDA, reaching a diam., of 7 cm after five days at 25 °C, effuse, velvety, with entire to slightly undulate edge. Surface initially white and later turning dark olivaceous from the surrounding of the colony and dark gray in reverse.

Materials examined.

China, Sichuan Province, Leshan City , Jingyan County, 29°30'27"N, 103°57'14"E, elevation 682 m, on dead branches of Idesia polycarpa , 23th July 2021, W.L Li, STZ 327 (HUEST 22.0095), living culture UESTCC 22.0094 ; ibid., STZ 359 (HUEST 22.0094), living culture UESTCC 22.0093 ; ibid., Leshan City, Shizhong Distinct , 29°42'13"N, 103°52'25"E, elevation 356 m, on dead branches of Paeonia suffruticosa , 23th July 2021, W.L Li, YMD 366 (HUEST 22.0096), living culture UESTCC 22.0095 ; ibid., Guangyuan City , Qingchuan County, 32°40'38"N, 105°28'57"E, elevation 638 m, on dead branches of Vernicia fordii , 20th April 2021, W.L. Li, YT 175 (HUEST 22.0097), living culture UESTCC 22.0096 .

Notes.

The morphology of our collections obtained from decaying woody oil plants are similar to the original description of Neofusicoccum parvum ( Crous et al. 2006). In the multi-gene phylogenetic analysis, these four isolates clustered together (ML/BI 75%/0.99) with the ex-type of N. parvum . Neofusicoccum parvum has a wide range of hosts and has a worldwide distribution ( Phillips et al. 2013). This is the first report of N. parvum on Idesia polycarpa .

Figures

Figure 15. Neofusicoccum parvum (HUEST 22.0097, new host record) a, b appearance of ascomata on natural substrate c, d vertical section of ascomata e ostiole f section of peridium g-j ascospores k, l immature asci m, n mature asci. Scale bars: 100 μm (d); 25 μm (e, f); 10 μm (g-j); 20 μm (k-n).