Collybiopsis melanopus (A.W. Wilson, Desjardin & E. Horak) R.H. Petersen,, 2021

Kumla, Wiphawanee Phonrob Jaturong, Suwannarach, Nakarin, Khuna, Surapong, Nutaratat, Pumin, Wannathes, Nopparat & Lumyong, Saisamorn, 2024, Five new records of Collybiopsis (Agaricales, Omphalotaceae) in Thailand: a significant addition to the species diversity and global distribution of the genus, Phytotaxa 650 (3), pp. 213-235 : 228-229

Collybiopsis melanopus (A.W. Wilson, Desjardin & E. Horak) R.H. Petersen,

Mycotaxon 136(2): 342 (2021) FIGURE 7 Index Fungorum number: IF556191

Pileus 12–20 mm diam., convex, expanding to plano-convex, rugulose-striate surface dull, dry, glabrous, disc brownish orange (7C5–7), margin paler to light orange (6A3–4) to beige (5A3). Context up to 1 mm thick, soft. Lamellae adnate, close to sub distant with 2–3 series of lamellulae, narrow, pale cream (4A3). Stipe 21–38 × 1.5–2 mm, central, cylindrical to tapering upwards, fistulose-stuffed, tough, sub insititious; surface dull, dry, white-pruinose overall, apex brownish grey (5–6C3), base dark brown to black; rhizomorphs absent. Odor and taste indistinct.

Basidiospores [25/1/1] 7–8.5 × 3.5–4.5 μm, [x̄ = 7.72 ± 0.56 × 3.9 ± 0.28 µm, Q = 1.75–2.43, Q m = 1.98 ± 0.19], ellipsoid, smooth, hyaline, inamyloid, thin-walled. Basidia 23–30 × 5–7 μm, clavate, smooth, hyaline, inamyloid. Pleurocystidia absent. Cheilocystidia 43–68 × 21–28 μm, clavate to fusiform, broadly obtuse, hyaline, inamyloid, thin-walled. Pileipellis a cutis of radially arranged, repent hyphae 4–6 μm, cylindrical, non-diverticulate, smooth or brown-incrusted, hyaline, inamyloid, non-gelatinous, thin-walled; terminal cells undifferentiated. Pileus trama radially arranged to interwoven; hyphae similar to pileipellis but up to 10 μm diam. Lamellar trama regular; hyphae 3.5–16 μm diam., cylindrical to inflated, hyaline, inamyloid, thin-walled. Stipitipellis a cutis with numerous clustered caulocystidia; cortical hyphae 2.5–8 μm diam., cylindrical, non-diverticulate, smooth or weakly brown-incrusted, hyaline to brown, inamyloid, thin-walled; medullary hyphae 4–16 μm diam., cylindrical to inflated, smooth, hyaline, inamyloid, thin-walled. Caulocystidia 43–54 × 5.5–9 μm, irregularly cylindrical to clavate, flexuose, hyaline, inamyloid, thin-walled. Clamp connections are present in all tissues.

Habitat and known distribution:—Fruiting scattered to gregarious on bark. Known from Africa ( São Tomé) ( Desjardin & Perry 2017), China (Hu et al. 2022), India ( Dutta et al. 2015), Indonesia (Java) (Desjardin et al. 2004), and Thailand (This study).

Material examined:— THAILAND, Chiang Mai Province, Chiang Mai University, 18°48´7´´N 98°57´23´´E, elevation 330 m, on bark, 10 August 2022, W. Phonrob, SDBR-CMUWP072.

Notes:—Thai materials have macroscopic and microscopic features well agree with descriptions of previous studies (Desjardin et al. 2004, Dutta et al. 2015, Desjardin & Perry 2017). Thai materials were grouped with C. melanopus with strong statistical support (BS = 100% and PP 1.00) ( FIGURES 2 and 3 ). Collybiopsis melanopus forms a sister clade to C. juniperina ; however, C. juniperina differs from C. melanopus by its darker reddish-brown pileus, wider basidiospores (7.2–9.6 × 4.0–5.2 μm) and presence of diverticula of pileipellis ( Mata et al. 2004b). In addition, C. juniperina was distributed in Argentina, Jamaica, and the USA ( Horak 1968, Singer 1973, Mata et al. 2004b, Petersen & Hughes 2016, 2021).

Figures

FIGURE 2. Phylogenetic tree derived from maximum likelihood analysis of 95 sequences of the combined ITS and nrLSU genes. Sequences of Rhodocollybia maculata TFB14382 and R. butyracea TFB13989 were used as outgroups. The numbers above the branches represent bootstrap percentages (left) and posterior probabilities (right). Bootstrap values ≥ 75% and posterior probabilities ≥ 0.90 are shown. The scale bar represents the expected number of nucleotide substitutions per site. Sequences of specimen vouchers obtained in this study are highlighted in red. Type species are shown in bold.

FIGURE 3. Phylogenetic tree derived from maximum likelihood analysis of 95 ITS gene sequences.Sequences of Rhodocollybia maculata TFB14382 and R. butyracea TFB13989 were used as outgroups. The numbers above the branches represent bootstrap percentages (left) and posterior probabilities (right). Bootstrap values ≥ 75% and posterior probabilities ≥ 0.90 are shown. The scale bar represents the expected number of nucleotide substitutions per site. Sequences of specimen vouchers obtained in this study are highlighted in red. Type species are shown in bold.

FIGURE 7.Collybiopsis melanopus (SDBR-CMUWP072).A.Basidiomata;B.Basidiospores; C. Basidia;D. Cheilocystidia;E. Pileipellis; F. Caulocystidia. Scale bars: A = 10 mm; B–F = 10 µm.