Mideopsis roztoczensis Biesiadka & Kowalik, 1979

Pešić, Vladimir, Zawal, Andrzej, Ferreira, Sónia, Benitez-Bosco, Laura, Cruz-Oliveira, Ana, Girão, Dinis, Padilha, Adriana, Turaccio, Paolo, Rossini, Samantha, Ballini, Lorenzo, Staffoni, Giorgia, Fratini, Sara, Ciofi, Claudio, Iannucci, Alessio, Ekrem, Torbjørn & Stur, Elisabeth, 2024, DNA barcode library of Portuguese water mites, with the descriptions of two new species (Acari, Hydrachnidia), ZooKeys 1217, pp. 119-171 : 119-171

Mideopsis roztoczensis Biesiadka & Kowalik, 1979

Fig. 8

Material examined.

Portugal, Beja: • Mértola, Moinho de Alferes 1 , 37.502°N, 7.69°W, 19 May 2023, leg. Ferreira, Benitez-Bosco, Ekrem, Stur & Turaccio, 1 ♀ (sequenced) ; Mértola, Pulo do Lobo , 37.805°N, 7.633°W, 18 May 2023, leg. Ferreira, Benitez-Bosco, Ekrem, Stur & Turaccio, 5 ♀, 1 deutonymph (sequenced) ; • Odemira, Ribeira de Seixe, Zambujeira do Mar , 37.398°N, 8.68°W, 75 m a.s.l., 23 May 2023, leg. Ekrem & Benitez-Bosco, 1 ♂ (sequenced) . Bragança • Vinhais, Gasparona , 41.85°N, 7.013°W, 683 m a.s.l., 6 Jul. 2023, leg. Ferreira & Padilha, 1 ♀, 1 deutonymph (sequenced) . Guarda: • Seia, Casa do Loureiro , 40.433°N, 7.701°W, 415 m a.s.l., 19 Jul. 2023 leg. Ferreira & Padilha, 1 ♂, 3 ♀, 1 deutonymph (sequenced) ; • Manteigas, Zêzere, Covão da Ametade , 40.328°N, 7.587°W, 1431 m a.s.l., 21 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♂, 1 ♀, 1 deutonymph (sequenced) ; • Manteigas, Zêzere, Ponte dos Frades , 40.403°N, 7.526°W, 672 m a.s.l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco, Padilha, Andrade & Stur 1 ♀ (sequenced) ; • Manteigas, Poço do Inferno , 40.373°N, 7.516°W, 1078 m a.s.l., 21 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♂, 1 ♀ (juv.), 1 deutonymph, 1 ♂ dissected and slide mounted ( RMNH) ; • Gouveia, Rio Mondego, Casais de Folgosinho , 40.454°N, 7.493°W, 976 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♂, 1 ♀ (sequenced) ; • Manteigas, Poio do Leão , 40.399°N, 7.541°W, 734 m a.s.l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♂ (sequenced) ; • Seia, Rio Alva, Central hidroelétrica de Ponte dos Jugais , river, 40.385°N, 7.706°W, 555 m a.s.l., 23 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♂, 2 ♀, 2 deutonymph (sequenced), 1 ♂ dissected and slide mounted ( RMNH) ; • Rio Alva, Nossa Senhora do Desterro , river, 40.395°N, 7.694°W, 791 m a.s.l., 23 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♀ (sequenced) ; • Manteigas, Mondego, Covão da ponte , 40.443°N, 7.514°W, 999 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♂ (sequenced) ; • Seia, Rio Alva, Praia Fluvial de Sabugueiro , river, 40.401°N, 7.64°W, 1021 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♀ (sequenced) ; • Manteigas, Casa do Cantoneiro , 40.418°N, 7.603°W, 1378 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♂, 1 ♀ (sequenced) . Vila Real • Murça, Noura stream , 41.409°N, 7.417°W, 421 m a.s.l., 12 Jul. 2023 leg. Ferreira S, Padilha 1 ♀ (sequenced) .

Remarks.

The specimens examined in our study match the description of Mideopsis roztoczensis , when following Biesiadka and Kowalik (1979). Mideopsis roztoczensis is characterized by a more elevated dorsal shield with distinctly visible anteriorly diverging lines of particularly faint fine porosity, and by the the shape of the male ejaculatory complex with the wedge-shaped anterior ramus being wider and with a characteristic arrow-shaped delimited area. Recently, Pešić et al. (2023 e) showed that M. roztoczensis is a genetically variable species, comprising four BINs ( BOLD: ACI 1492, BOLD: AEN 6785, BOLD: AEA 2936, BOLD: AEO 2944) widely distributed in Europe. In this study, we detected an additional five unique BINs ( BOLD: AFU 6108, BOLD: AFP 5421, BOLD: AFW 3785, BOLD: AFV 6334, BOLD: AEV 2909) within the Portuguese M. roztoczensis - like specimens (Fig. 8 ), all of them unique, and some of them present at the same sites (e. g., BOLD: AFU 6108 and BOLD: AFW 3785 in Casa do Loureiro, BOLD: AFU 6108 and BOLD: AFV 6334 in Casa do Cantoneiro).

Distribution.

Europe, Turkey. New record for Portugal.

Figures

Figure 8. Neighbor-Joining tree of the genus Mideopsis obtained from 112 nucleotide COI sequences; 71 sequences were taken from Pešić et al. (2023 a) and 41 sequences from Portugal are newly generated in this study. Mundamella germanica from Montenegro was used as outgroup. BINs are based on the barcode analysis from 16 May 2024. Country codes: BA = Bosnia and Herzegovina, BE = Belgium, CY = Cyprus, DE = Germany, FR = France, ME = Montenegro, PT = Portugal, RS = Serbia, RU = Russia, TR = Turkey. Bootstrap values > 50 % from 1000 bootstrap replicates on branches.