Ulva sp.

Noor, Sadia, Begum, Mst. Nadira, Rony, Satyajit Roy, Uddin, Mohammad Zashim, Sohrab, Md. Hossain & Mazid, Md. Abdul, 2024, Bioactivity and chemical screening of endophytic fungi associated with the seaweed Ulva sp. of the Bay of Bengal, Bangladesh, Botanica Marina (Warsaw, Poland) 67 (2), pp. 115-129 : 117

2.4 Preparation of fungal crude extracts associated with Ulva sp.

Each endophytic fungal isolate was cultured and incubated at 28 ± 2 ° C in the dark. The culture medium was prepared by dissolving PDA (39 g l −1) in artificial seawater and autoclaving at 121 ° C for 15 min. After 21–28 days, the media with fungal metabolites were frozen at −20 ° C after observing the highest mycelial growth. When thawed, the aqueous part of the fungal culture was separated from the fungal mycelia by filtration and then extracted with chloroform three times using a separating funnel. The fungal mycelia were soaked with ethyl acetate. The EtOAc extract (organic part) was prepared by filtration after 7 days and subsequent solvent evaporation at 5-day intervals using a rotary evaporator at 40 ° C ( Chowdhury et al. 2017; Khan et al. 2016). The dried fungal crude extracts were kept at 4 ° C until further analysis.