Apiospora montagnei Sacc., Atti Soc. Veneto-Trent. Sci. Nat. 4: 85. 1875.

Pintos, Angel & Alvarado, Pablo, 2022, New studies on Apiospora (Amphisphaeriales, Apiosporaceae): epitypification of Sphaeria apiospora, proposal of Ap. marianiae sp. nov. and description of the asexual morph of Ap. sichuanensis, MycoKeys 92, pp. 63-78 : 63

Apiospora montagnei Sacc., Atti Soc. Veneto-Trent. Sci. Nat. 4: 85. 1875.

Fig. 2C-2N

Sphaeria apiospora Durieu & Mont., Expl. Sci. Alg., Fl. Algér. 1, livr. 13: 492. 1849. [replaced name]

Hypopteris apiospora (Durieu & Mont.) Berk., Hooker’s J. Bot. Kew Gard. Misc. 6: 227. 1854.

Arthrinium phragmitis Crous, IMA Fungus 4: 147. 2013.

Apiospora phragmitis (Crous) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 206. 2021.

Sexual morph.

Stromata solitary to gregarious, immersed to erumpent, fusiform, with the long axis broken at the top by one or two cracks, (0.5-)2.1-2.9(-4) × (0.2-)0.25-0.35(-0.5) mm (n = 20). Ascomata uniseriate or irregularly arranged beneath stromata, pseudothecial, black, globose to subglobose with a flattened base, (150-)159-183(-200) µm high × (200-)247-278(-300) µm wide (n = 35), with a conspicuous periphysate ostiole. Peridium composed of 5 or 6 layers of brown to hyaline cells arranged in textura angularis. Hamathecium paraphyses hyphae-like, up to 4 µm wide. Asci broadly cylindrical, clavate, with an indistinct pedicel, rounded at the apex, lacking apical apparatus, (72-)99-111(-115) × (14-)15.5-16.5(-18) µm (n = 25). Ascospores uniseriate or biseriate, clavate to fusiform, straight or slightly curved, with narrowly rounded ends, composed of a large upper cell and a small lower cell, hyaline, smooth-walled, measuring (21-)23-24.5(-25) × (6-)6.3-7.1(-8) µm (n = 30).

Asexual morph.

Mycelium consisting of hyaline, smooth, branched, septate hyphae 1-4 μm in diam. (n = 20). Conidiophore mother cell from hyaline to brown, solitary or aggregated in groups on hyphae, subsphaerical to lageniform or ampuliform, measuring (4-)6.6-8(-10) × (3-)4.5-5.1(-6) µm (n = 10). Conidiophores cylindrical, straight to flexuous, some of them branched, hyaline, measuring (10-)18-34(-45) × (1.5-)1.6-1.8(-2) µm (n = 20). Conidiogenous cells doliiform to lageniform or ampuliform, hyaline, measuring (10-)11.5-13.1(-15) × (2-)4.3-5.1(-6) µm (n = 20). Conidia ellipsoidal to ovoid, smooth to finely roughened, with an equatorial germ slit of paler pigment, measuring (9-)10.3-11.3(-12) µm in surface view, (5-)6.2-7.2(-8) µm in side view (n = 25). Sterile cells ellipsoidal to clavate, measuring 13-16 µm (n = 25).

Culture characteristics.

Colonies flat, spreading, with moderate aerial mycelium. On MEA, surface dirty white with pale rose patches, reverse luteous. Occupying an entire 90 mm Petri dish in 14 days at room temperature, sporulating four weeks after culture.

Epitype.

Spain: Catalonia, Girona, L´Escala, on Arundo micrantha , 30 November 2020, leg. Marc Grañem, AP301120 (epitype selected here PC:0125164, ex-type culture CBS 148707; iso-epitype WU-MYC0044524, ex-type culture CBS 148708).

Other specimens examined.

Spain: Balearic Islands, Mallorca, Esporlas , on Arundo donax , 14 December 2020, leg. Ángel Pintos, AP141220 (WU-MYC0044527) . Balearic Islands, Mallorca, Palma de Mallorca, Torrente de Establiments , on Arundo micrantha , 19 April 2021, leg Ángel Pintos, AP 19421 (WU-MYC0044523) . Balearic Islands, Mallorca, Puerto de Alcudia , on Arundo donax , 2 April 2021, leg. Ángel Pintos, AP 2421 (WU-MYC0044522) . Balearic Islands, Mallorca, Puerto de Andratx , on Arundo donax , 5 April 2021, leg. Ángel Pintos, AP 5421 (WU-MYC0044529) . Balearic Islands, Mallorca, Puerto de Soller , on Arundo donax , 3 April 2021, leg. Ángel Pintos, AP 3421 (WU-MYC0044528) . Balearic Islands, Mallorca, Puigpunyent , on Arundo micrantha , 11 December 2020, leg. Ángel Pintos, AP111220 A. Balearic Islands, Mallorca, Soller , on Arundo donax , 4 April 2021, leg. Ángel Pintos, AP 4421 (WU-MYC0044530) . Balearic Islands, Mallorca, Universitat de les Illes Balears (UIB), on Arundo donax , 28 December 2020, leg. Ángel Pintos, AP281220 (WU-MYC0044521) . Catalonia, Barcelona, Premia de Dalt , on Arundo donax , 10 October 2020, leg Miguel Mir, AP101020 . Catalonia, Girona, Bescanó, on Arundo micrantha , 29 November 2020, leg. Marc Grañem, AP291120 (WU-MYC0044526).

Notes.

The phylogenetic boundaries of Apiospora were recently discussed by Pintos and Alvarado (2021), who selected a lectotype (PC:0125160) for S. apiospora , the basionym of the type species Ap. montagnei . In the present study, an epitype (PC:0125164) of Ap. montagnei is selected among modern collections growing on the same host in Girona, Spain (about 100 km south of the type locality, Perpignan, France). All samples growing on the same host collected in Girona or the Balearic Islands (Spain) are genetically identical to the epitype, and match the phylogenetic clade formerly known as Ap. phragmitis , excepting one that matches Ap. sichuanensis , but the ascospores of this species (29-48 × 7-10.5 μm, Samarakoon et al. 2022) clearly exceed those of Ap. montagnei (21-25 µm, Pintos and Alvarado 2021). Therefore, on the basis of these results, it is here hypothesized that Ap. montagnei is a prioritary synonym of Ap. phragmitis .

Figures

Figure 2. A, B Gymnosporium arundinis (original material from PRM) A substrate of the type B label of the original collection of Gymnosporium arundinis C-N Apiospora montagnei (AP 301120) C stromata on host D, E asci and periphyses F-I ascospores J-L conidiogenous cell with conidia M-N conidia in face and side view. Scale bars: 100 µm (C); 5 µm (D-I); 10 µm (J-N).