Nothophoma ngayawang E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan, 2021

Keirnan, Elizabeth C., Tan, Yu Pei, Laurence, Matthew H., Mertin, Allison A., Liew, Edward C. Y., Summerell, Brett A. & Shivas, Roger G., 2021, Cryptic diversity found in Didymellaceae from Australian native legumes, MycoKeys 78, pp. 1-20 : 1

Nothophoma ngayawang E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan sp. nov. Fig. 6

Type.

Australia, South Australia, Blanchetown, Senna artemisioides , 22 Oct. 2016, E.C. Keirnan, holotype BRIP 69582 (includes culture ex-type).

Description.

Colonies on OA, 18-20 mm diam. after 7 d, covered by scant tufted aerial mycelia at centre becoming abundant and floccose towards margin, rosy buff becoming darker towards centre; reverse salmon with centre and margins pale isabelline; on MEA, 15-20 mm after 7 d, margin irregular, felty buff becoming white towards the margin; reverse pale rosy buff, darker at centre becoming paler near margin; on PDA, 18-21 mm after 7 d, margin regular, aerial mycelia tufted in centre becoming floccose toward the margin, white to pale rosy buff; reverse pale rosy buff with few scattered vinaceous spots. NaOH spot test: slightly yellow. Conidiomata pycnidial, globose to subglobose, 200-300 μm diam., pale brown becoming black, solitary, abundant in centre of colony, glabrous, non-papillate; ostiole c. 25 μm diam.; pycnidial wall composed of textura globulosa, pale brown, cells 5-8 μm diam. Conidiogenous cells phialidic, cylindrical, thin-walled, hyaline. Conidia aseptate, 2.5-4.0 × 1.0-2.0 μm, cylindrical to narrow ellipsoidal, hyaline, thin-walled.

Etymology.

Named after the Indigenous Australian Ngayawang people’s language group, who existed in the Murray River region of South Australia, which includes Blanchetown, the locality where this specimen was collected.

Notes.

Nothophoma ngayawang is phylogenetically close to No. anigozanthi ex-type strain CBS 381.91 (Fig. 2 ). Nothophoma ngayawang is distinguished from No. variabilis by the ITS region (98 % identity) and the rpb 2 locus (93% identity). The NaOH spot test of No. variabilis was negative on MEA, which is distinguished from the slightly yellow reaction of No. ngayawang .

Figures

Figure 2. Epicoccum djirangnandiri: a leaf lesions on Swainsona galegifolia b 14 - d old colonies on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e conidia. Scale bars: 200 µm (d); 7 µm (e).

Figure 6. Nothophoma ngayawang: a leaf and pod lesions on Senna artemisioides b 14 - d old colonies, top to bottom on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia e pycnidial wall f conidia. Scale bars: 250 µm (d); 8 µm (e); 3 µm (f).