Kodamaea ovata C.Y. Chai & F.L. Hui, 2022

Chai, Chun-Yue, Gao, Wan-Li, Li, Ying, Yan, Zhen-Li & Hui, Feng-Li, 2022, Kodamaea hongheensis f. a., sp. nov., Kodamaea ovata f. a., sp. nov. and Kodamaea yamadae f. a., sp. nov., three new yeast species of Kodamaea (Saccharomycetales, Debaryomycetacae) from China, MycoKeys 89, pp. 121-137 : 121

Kodamaea ovata C.Y. Chai & F.L. Hui sp. nov.

Fig. 3

Holotype.

China, Henan Province, Nanyang City, the Baotianman Nature Reserve, in rotting wood under a mixed forest, July 2016, K.F. Liu & Z.W. Xi (holotype NYNU 167144T, ex-holotype CBS 14702).

Etymology.

The specific epithet Kodamaea ovata refers to the ovoid cell morphology of the type strain.

Description.

In YM broth, after 3 days at 25 °C, cells are ovoid (2-4 × 3-5 μm) and occur singly or in pairs (Fig. 3a ). Sediment is formed after a month, but a pellicle is not observed. On YM agar, after 3 days at 25 °C, colonies are white to cream-colored, butyrous and smooth with entire margins. In Dalmau plate culture on CM agar, a rudimentary pseudomycelium is formed (Fig. 3b ). Asci or signs of conjugation are not seen on sporulation media. Glucose, galactose, maltose and trehalose are fermented but sucrose, melibiose, lactose, cellobiose, melezitose, raffinose, inulin and xylose are not. Glucose, galactose, glucosamine, d-xylose, d-arabinose, l-arabinose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, arbutin, melezitose, inulin, glycerol, ribitol, xylitol, d-glucitol, d-mannitol, galactitol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, dl-lactate, succinate, citrate and ethanol are assimilated as sole carbon sources. l-sorbose, d-ribose, l-rhamnose, melibiose, lactose, raffinose, erythritol, myo -inositol, d-gluconate, d-glucuronate and methanol are not assimilated. Ethylamine, l-lysine and creatine are assimilated as sole nitrogen sources. Nitrate, nitrite, cadaverine, creatinine, glucosamine, imidazole and d-tryptophan are not assimilated. Growth is observed at 42 °C but not at 45 °C. Growth in the presence of 0.1% cycloheximide and 16% NaCl plus 5% glucose is positive, but growth in the presence of 1% acetic acid is negative. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolate examined.

China, Henan Province, Nanyang City, the Baotianman Nature Reserve, in rotting wood under a mixed forest, July 2016, K.F. Liu & Z.W. Xi (NYNU 1685).

Notes.

Two strains NYNU 1685 and NYNU 167144 representing K. ovata grouped in a well-supported clade and appear to be most closely related to K. neixiangensis ( Gao et al. 2017). The nucleotide differences between the new species and the close relative K. neixiangensis are 1% substitutions in the LSU rDNA sequence and 4.8% substitutions in the ITS region, respectively. Physiologically, K. ovata can be differentiated from K. neixiangensis based on growth in l-arabinose, d-arabinose, dl-lactate and 16% NaCl plus 5% glucose, all of which were positive for K. ovata and negative for K. neixiangensis . Additionally, the new species ferments galactose and maltose and grows at 35 °C, but K. neixiangensis does not have these characteristics.

Figures

Figure 3. Morphology of Kodamaea ovata (NYNU 167144, holotype) a budding cells in YM broth after 3 d b pseudohyphae on CM agar after 14 d. Scale bars: 10 μm.