Cladosporium tenuissimum Cooke, Grevillea 6: 140 (1878)

Wang, Si-Yao, Wang, Yong & Li, Yan, 2022, Cladosporium spp. (Cladosporiaceae) isolated from Eucommia ulmoides in China, MycoKeys 91, pp. 151-168 : 151

Cladosporium tenuissimum Cooke, Grevillea 6: 140 (1878)

Fig. 6a-i

Materials examined.

China, Guizhou Province, Guiyang, Huaxi district , plantation forest of E. ulmoides , Guizhou University (26°24'16"N, 106°40'29"E), on fallen leaves of E. ulmoides , S.Y. Wang, Y. Wang & Y. Li, 13 January 2021, (HGUP 401.1; HGUP 401.2; HGUP 401.3 and HGUP 401.4, living cultures GUCC 401.2; GUCC 401.3; GUCC 401.4 and GUCC 401.5) (new substrate record) .

Description.

Saprobic on fallen leaves of Eucommia ulmoides . Sexual morph: Not developed. Asexual morph: Hyphomycetous. Mycelium abundant, superficial and submerged, overgrowing whole culture dishes, hyphae straight to slightly sinuous, septate, branching, light olive-green to olive-brown, smooth-walled, 1.5-6 µm wide. Conidiophores 13-100 × 2.5-4.5 µm (x̄ = 60.8 × 3.6 µm; n = 10), erect, branching, light olive-green, smooth and thin walled. Conidia 2.5-7.5 × 2-4 µm (x̄ = 4.9 × 3.2 µm; n = 30), in simple and branched acropetal chains, mostly light olive-green, aseptate, mostly smooth- and thin-walled, variable in size and shape, subglobose, ellipsoid-ovoid, obovoid, fusiform. Secondary ramoconidia 5.5-23 × 2.5-5.5 µm (x̄ = 0.9 × 3.8 µm; n = 30), pale olive-green, narrowly ellipsoid to cylindrical-oblong or subcylindrical, sometimes septate and sometimes aspetate (1-septate appear at maturity), smooth- and thin-walled.

Culture characteristics:

Colonies on SNA 50-55 mm diam, after 2 weeks at 25 °C, pale olive to pale white, flat, velvety, with a regular edge, reverse light olive to light white. Colonies on PDA 40-55 mm diam, after 2 weeks at 25 °C, smoke-gray to light olive-gray or olive to light olive-gray, reverse leaden-gray, gray-olive at edge both surface and reverse, woolly or felty, broad edge, regular, growth low convex, without protuberant exudates, occasionally reverse formed a sunflower like shape in the middle. Colonies on MEA 40-50 mm diam, after 2 weeks at 25 °C, olive-gray or gray, fluffy; reverse olive-green to dark olive, with an olive-yellow to gray-white edge, radially furrowed. Colonies on OA 40-60 mm diam, after 2 weeks at 25 °C, gray-white or iron-gray to gray-olive, fluffy to felty; reverse olive-brown to olive.

GenBank numbers.

ITS: OL579746, tef1: OL504967, act: OL519776 (GUCC 401.2); ITS: OL579745, tef1: OL505077 (GUCC 401.3); ITS: OL579744, tef1: OL581724, act: OL519777 (GUCC 401.4); ITS: OL579743, tef1: OL581725, act: OL519778 (GUCC 401.5).

Figures

Figure 6. Cladosporium tenuissimum (GUCC 401.2, new substrate record from Guizhou Province). a-d colonies on SNA, PDA, MEA and OA (left: above, right: reverse) e-h secondary ramoconidia and conidia on SNA i conidia on SNA. Scale bars: 10 µm (e-i).