Phyllosticta guangdongensis Ning Jiang & C.B. Wang, 2023

Wang, Cheng-Bin, Yang, Jing, Li, Yong, Xue, Han, Piao, Chun-Gen & Jiang, Ning, 2023, Multi-gene phylogeny and morphology of two new Phyllosticta (Phyllostictaceae, Botryosphaeriales) species from China, MycoKeys 95, pp. 189-207 : 189

Phyllosticta guangdongensis Ning Jiang & C.B. Wang sp. nov.

Fig. 3

Etymology.

Referring to the Guangdong Province, where the species was first collected.

Description.

Sexual morph: Unknown. Asexual morph: Conidiomata pycnidial, aggregated, black, globose to pyriform, exuding opaque conidial masses, erumpent, 100-450 µm diam. Conidiophores subcylindrical to ampulliform, reduced to conidiogenous cells. Conidiogenous cells phialidic, subcylindrical to ampulliform, hyaline, smooth, 10-15 × 2.5-4 μm. Conidia 10-14 × 6-8 μm, (mean ± SD = 11.5 ± 1.3 × 7.5 ± 0.6 μm), solitary, hyaline, aseptate, thin and smooth-walled, ellipsoid to obovoid, coarsely guttulate, enclosed in a thin persistent mud sheath, 1-1.5 μm thick, with an apical mucoid appendage, 4.5-10 × 1-2 μm, flexible, unbranched, tapering towards an acutely rounded tip.

Culture characters.

Colonies on PDA flat, slow growing, grayish-green in the center, and dark green at margin reaching 85 mm diameter after two weeks. Colonies on MEA slow growing, yellow in the center, white at undulate the margin, reaching a 20-25 mm diameter after two weeks. Colonies on SNA flat, slow growing, grayish-green, reaching a 25-30 mm diameter after two weeks.

Specimens examined.

China, Guangdong Province, Guangzhou City, leaf spot of Viburnum odoratissimum , Yong Li , 20 September 2022 (holotype CAF800073; ex-type culture: CFCC 58144). Ibid. (cultures: CFCC 58766 and CFCC 58772) .

Notes.

Phylogeny indicates that P. anhuiensis groups sister to P. mangiferae (IMA 260576). P. mangiferae was associated with Mangifera indica leaves in Tanzania ( Ebbels and Allen 1979; Glienke et al. 2011). Comparison of DNA sequences of P. anhuiensis with P. mangiferae (IMA 260576), there are 99.1% (471/475 identifies, 0/475 gaps) sequence similarity in ITS, 99.6% (760/763 identifies, 0/763 gaps) in LSU, 97.7% (211/216 identifies, 2/218 gaps) in tef1, 98.2% (221/225 identifies, 0/225 gaps) in act, and 98.4% (614/624 identifies, 6/624 gaps) in gapdh. Morphologically, P. guangdongensis can be distinguished from P. mangiferae in longer conidia (10-14 μm in P. guangdongensis vs. 8-12 µm in P. mangiferae ) and shorter appendage (4.5-10 µm in P. guangdongensis vs. 7-13 µm in P. mangiferae ) ( Glienke et al. 2011). Therefore, this species was regarded as a new species based on morphology and multi-locus phylogenetic analyses.

Figures

Figure 3. Morphology of Phyllosticta guangdongensis (CFCC 58144) A diseased leaf of Viburnum odoratissimum B conidiomata C conidiogenous cells D, E conidia F-H colonies on PDA, MEA and SNA after two weeks at 25 ° C. Scale bars: 500 μm (B); 10 μm (C-E).