Haplocampa wagnelli Sendra

Sendra, Alberto & Wagnell, Craig, 2019, The cave-dwelling dipluran (Diplura, Campodeidae) on the edge of the Last Glacial Maximum in Vancouver Island caves, North America (Canada), Subterranean Biology 29, pp. 59-77 : 60-64

Haplocampa wagnelli Sendra sp. n. Figs 1-5, 6-10, 11, 12, 13-16, 17-21, 22, 23, 24, 25, 26, 27; Tables 1, 2; Suppl. material 1

Etymology.

This species is dedicated to the co-author of this article, a caver who has dedicated many years sampling and exploring in Vancouver Island caves.

Type material.

Female holotype labeled ♀01 from Kiku Pot Cave, Port Alberni, Vancouver Island, Canada, 5th August 2018, C. Wagnell leg. (SEHU); 1 ♂ labeled ♂01, paratypes from Fossli Slots Caves, Port Alberni, Vancouver Island, Canada and 4 females labeled ♀02-♀05 from Fossli Slots Caves, Vancouver Island Canada, 15th July 2018, C. Wagnell leg. All type material mounted in Marc André solution. Deposited in AS collection.

Other studied material.

Four specimens from Fossli Slots Caves, 8th June 2018, C. Wagnell leg as type material mounted in separated aluminum stages and coated with palladium-gold. Deposited AS collection.

Description.

Body length 4.4 mm (male) and 3.4-6.0 mm (females). Epicuticle smooth under optical microscope but reticulated in high magnifications in round polygonal structures variable in size (Figs 4, 14-15); rosette gland formations present along the body (Figs 5, 16); body with abundant short and smooth clothing setae.

Moliniform antennae. Every intact antenna in the six type specimens has 32 antennomeres; from 0.5 to 0.7 times longer than body in larger and smaller adults, respectively. First antennomere four times shorter than second antennomere, and apical antennomere 1.3 longer than wide (Fig. 1); the other antennomeres, as long as wide (Fig. 7).

Cupuliform organ occupying 1/3 of the total length of the apical antennomere with about five complex olfactory chemoreceptors, each one a multiperforated–folded spiral structure, all tightly packed in the narrow open space of the cupuliform organ (Figs 2, 3). Distal and central antennomeres with a sensorial equipment: one whorl of bifurcated macrosetae, two to three whorls of untidy setae, and a single distal whorl of 10-13 short and thick gouge sensilla of 14-16 µm long 2-3 very short grooved closed–bud form sensilla 6-8 µm long; this sensorial equipment is also present in the apical antennomere (Figs 4, 6-10). Proximal antennomeres with typical trichobothria plus a thick and long sensillum on the third antennomere, located in ventral position, although the ventral c and d macrosetae are not differentiated.

Head subtrapezoidal with slightly protuberant lateral posterior angles (Fig. 11). Plain frontal process with the slightly differenced frontal smooth macrosetae (Fig. 12). From the three macrosetae along each side of the line of insertion of antennomere and x setae, a macrosetae is not differentiated and i/p/x have 36/57/23 relative lengths, all smooth (Fig. 12). Suboval labial palps with latero-external long thick sensillum, with two guard setae, up to 8 setae on anterior border and up to 80 neuroglandular setae.

Thoracic macrosetae distribution (Fig. 13): pronotum with 1+1 ma, 1+1 la, 1+1 lp; mesonotum with 1+1 ma, 1+1 la, 2+2 lp2, 3; metanotum with 1+1 ma, 1+1 lp2 macrosetae. All macrosetae relatively well developed, long with thin long barbs; marginal setae slightly longer and thicker than clothing setae and also smooth. Legs very slightly elon gated, metathoracic legs reaching the VIII abdominal segment in smaller adults and the VI in larger ones. Femur and tibia similar in length but tarsus clearly shorter and thicker (tibia/tarsus ratio 0.6 to 0.7) (Table 1). Femur I–III with one dorsal macrosetae well differentiated with a few thin long barbs in its distal half. Calcars with a few thin, long barbs throughout. Tibia I–III with two or three ventral macrosetae bifurcated in the apex (Fig. 17). Three or two dorsal, lateral and sometimes ventral tarsal setae similar to clothing setae but much longer (Fig. 18). Unequal claws (posterior claw 1.5 longer than anterior) with large lateral crests and no lateral process; ventral side of the claws noticeably ridged and covered by a micro-granulation surface; a spiny protrusion is visible in the basal portion of both claws (Figs 18-21); posterior claw with a large backward overhang (Figs 18, 19).

Distribution of abdominal macrosetae on urotergites (Fig. 22): 1+1 ma on I-III; 1+1 ma, 1+1 lp2 on IV; 1+1 ma, 1+1 la and 2+2 lp2,3 on V-VII: 1+1 ma and 3+3 lp1,2,3 on VIII; 1+1 ma (slightly backwards) and 5+5 lp1,2,3,4,5 on IX abdominal segment. All tergal abdominal macrosetae long and well differentiated with thin barbs along the half to third distal.

Urosternite I with 6+6 macrosetae (Figs 23-24); II to VII with 4+4 macrosetae; VIII with 1+1 macrosetae; short to middle size urosternal macrosetae, bi or trifurcated or with long barbs. Short styli with smooth short apical setae with two long teeth; bifurcated subapical and ventromedial setae (Fig. 25). Cerci half shorter than the body length, from 0.64 × in the smaller adults to 0.47 × in the holotype; so, the length of the cerci increases disproportionally with the body size. As well, the length of the articles increases from proximal to distal. Basal article is divided into three to eight secondary articles, followed by five to six primary articles bearing from two to five whorls of bifurcated macrosetae, and three to six smooth setae including the distal setae whorl in each primary article (Table 2; Figs 26, 27).

Female urosternite I with short subcylindrical appendages, each bearing up to 30 a1-glandular setae in a distal field (Fig. 23).

Male urosternite I with short moderately thick subcylindrical appendages, each bearing about 50 a1-glandular setae in a large field; up to setae covered the with two to three rows the posterior part of the first urosternite (Fig. 24).

Remarks.

The most visible features are the slightly troglomorphic characteristics of H. wagnelli sp. n., as shown by its antennae with 32 antennomeres, a record within the genus Haplocampa but a moderate antennomere number in a troglomorphic campodeid; and the five complex olfactory chemoreceptors, each one a multiperforated, folded-spiral structure However, any comparison with other Haplocampa species is currently impossible since these sensilla have not been described in any other species of the genus. The closest species to H. wagnelli sp. n. is H. rugglesi from Mount Rainier (Washington, USA), with equal macrosetae distribution on nota and similar on urotergites. Nevertheless, some taxonomic features are unique to H. wagnelli sp. n., such as medial anterior macrosetae on urotergites and three tibial ventral macrosetae. Many taxonomical details remain incomparable what is known described species, due to the lack of high magnifications.