Seifertia shangrilaensis J.F. Li, Phookamsak & K.D. Hyde, 2016

Li, Junfu, Phookamsak, Rungtiwa, Mapook, Ausana, Boonmee, Saranyaphat, Bhat, Jarayama D., Hyde, Kevin D. & Lumyong, Saisamorn, 2016, Seifertia shangrilaensis sp. nov. (Melanommataceae), a new species from Southwest China, Phytotaxa 273 (1), pp. 34-42 : 36-38

Seifertia shangrilaensis J.F. Li, Phookamsak & K.D. Hyde , sp. nov. Fig. 2

Index Fungorum number: IF552010; Facesoffungi number: FoF 01885

Etymology:—Refers to the location, where the holotype was collected.

Diagnosis:—Differs from Seifertia azalea in its shorter and hyaline to subhyaline conidia.

Holotype: MFLU 16-0267

Epiphytic on living rachides of Rhododendron decorum Franch. Sexual morph: Undetermined. Asexual morph: Synnemata 1000–2300 μm high, 120–200 μm wide, erect, simple, unbranched, dark brown to black, with bubblelike, tightly interwoven, branched hyphae, compacted into an elongate bundle. Mycelium superficial, partly immersed on the substrate, composed of septate, branched, smooth, thin-walled, pale white to white hyphae. Conidiophores (1000–) 1300–2100 (–2150) × (9.5–) 10–11 (–13) μm, (x = 1804.7 × 10.3 μm, n = 40), synnematous, closely packed into a bundle, hyaline to pale brown, thin-walled, smooth, septate, unbranched, straight or flexuous, cylindrical. Conidiogenous cells (5–) 7–11 (–12.5) × (4.5–) 5.5–6.5 (–7) μm, (x = 7.9 × 5.8 μm, n = 100), holoblastic, phialidic, integrated, terminal, determinate, subhyaline, cylindrical to subclavate, smooth. Conidia (2.5–) 3.5–5 (–6) × 2.5–3.5 μm (x = 4.2 × 3.1 μm, n = 100), phialoconidia, hyaline to subhyaline, fusiform to subglobose, or ellipsoidal, aseptate, borne in basipetally, developing pseudo-chains, clavate, smooth, thin-walled, sometimes aggregated into slimy masses at the apex of the synnema.

Cultural characteristics:— Conidia germinating on PDA within 14 hours at 15 ºC, germ tubes produced from apex. Colonies growing on PDA, reaching 5 mm diam. after 4 weeks at 25 ºC, mycelium semi-immersed to superficial, irregular in shape, flat, slightly raised, with undulate edge, slightly rough on surface, cottony to fairy fluffy, colony from above, initially write to cream at the margin, yellowish-brown in the centre, becoming white at the margin, dark brown at the centre after 4 weeks; from below, initially, cream at the margin, orangish-brown to reddish-brown at the centre, becoming dark brown to black after 4 weeks, producing brown pigmentation in agar.

Material examined:— CHINA, Yunnan Province, Shangrila, on living rachides of Rhododendron decorum ( Ericaceae ), 20 October 2014, R. Phookamsak, SgL027 ( MFLU 16-0267, holotype!; KUN-HKAS 93733, isotype!), ex-type living culture, MFLUCC 16-0238!, KUMCC 16-0002.

Known distribution:— widespread in temperate and boreal regions.

Notes: — Seifertia shangrilaensis is similar to the type species, Seifertia azalea in having narrow and pale conidia, with one or two conidiogenous loci of conidiogenous cells, dark conidiophores and erect, synnemata ( Partridge & Morgan-Jones 2002, Glawe et al. 2006, Seifert et al. 2007). However, S. shangrilaensis differs from S. azalea in having hyaline to subhyaline, and wider conidiophores (9.5–12.6 μm vs. 4–7 μm), and smaller conidia (2.5–6 × 2.5–3.6 μm vs. 4–12 × 4–8 μm).

Figures

FIGURE. 2. Seifertia shangrilaensis (MFLU 16-0267, holotype). a. Living rachides of Rhododendron decorum. b. Appearance of synnema on rachides. c. A synnema visualized under the compound microscope.d–h. Conidiogenous cells. i–p. Conidia. q–s. Germinating conidia. t. Multiple culture colonies from above. u. Multiple culture colonies. v. Single colony on PDA after 4 weeks. Scale bars: t–u = 2 cm, v = 1 cm, a = 0.5 cm, b = 500 μm, c = 200 μm, g–h, j–k, q–s = 10 μm, d–f, i, l–n = 5 μm.